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Phosphorylation-Induced Self-Coacervation versus RNA-Assisted Complex Coacervation of Tau Proteins.
J Am Chem Soc
March 2025
Institute of Biological Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei 115, Taiwan.
In this study, the role of phosphorylation in the liquid-liquid phase separation (LLPS) of tau, the underlying driving forces, and the potential implications of this separation on protein conformation and subsequent protein aggregation were investigated. We compared in vivo-produced phosphorylated tau (p-tau) and nonphosphorylated tau under different coacervation conditions without adding crowding agents. Our findings revealed that spontaneous phase separation occurs exclusively in p-tau, triggered by a temperature shift from 4 °C to room temperature, and is driven by electrostatic and hydrophobic interactions.
View Article and Find Full Text PDFCommunity pharmacy services in the late COVID-19 period: What has driven change?
Res Social Adm Pharm
March 2025
WHO Collaborating Centre for Pharmaceutical Pricing and Reimbursement Policies, Pharmacoeconomics Department, Gesundheit Österreich GmbH (GÖG / Austrian National Public Health Institute), Stubenring 6, 1010, Vienna, Austria; Department of Health Policy, London School of Economics and Political Science, Houghton Street, London, WC2A 2AE, UK. Electronic address:
Background: Community pharmacy appears to have undergone considerable change over the years.
Objectives: The objective of this research is to study the range of community pharmacy services provided in late stages of the COVID-19 pandemic and during the last decades and to identify potential drivers for change.
Methods: Four European countries (Austria, England, Estonia, and Portugal), which represent a balance in terms of income, organization of the health system and pharmacy services, were selected as case studies.
Hidden aging: the secret role of root senescence.
Trends Plant Sci
March 2025
Molecular Plant Nutrition, Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany. Electronic address:
Root age-dependent processes have remained poorly understood. Here, we define root age-related terms in their eco-/physiological context, provide a synthesis of read-outs and traits characterizing root senescence in different root types, and follow their modulation in the light of metabolic, hormonal, and genetic control. Evidence for an endogenously regulated senescence program in roots includes changes in root anatomy, metabolism, and color, decrease in root activity, increasing levels of stress-related hormones, and increasing expression of certain transcription factors (TFs) or genes involved in oxidative stress defense.
View Article and Find Full Text PDFIn situ global mapping of protein perturbations via protein abundance and conformation analysis.
Anal Chim Acta
May 2025
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 639 Longmian Dadao, Nanjing, 211198, China. Electronic address:
Background: Traditional studies of protein responses to external stimuli primarily focus on changes in protein abundance, often overlooking the critical role of protein conformational alterations. To address this gap, we developed Protein Abundance and Conformation Analysis (PACA), an integrative method that quantifies both protein abundance and conformational changes. PACA combines conventional quantitative proteomics for abundance measurements with Target Response Accessibility Profiling (TRAP), a technique that captures conformational changes in situ by applying reductive dimethylation to label accessible lysine residues in living cells before lysis.
View Article and Find Full Text PDFRatio fluorescence and smartphone-assisted colorimetry dual-mode detection of creatinine based on F, B, N-doped of red fluorescent carbon dots.
Anal Chim Acta
May 2025
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, PR China.
Background: Creatinine is a small molecule disease biomarker that reflects kidney function, accurate and effective detection of creatinine will play an important role in the prevention and treatment of diseases. Currently, commonly used creatinine detection methods are limited by expensive instruments, complex sample preparation, many interference factors from biological samples, and environmental factors that can affect the accuracy of the measurement. Therefore, developing a fast, simple, inexpensive, sensitive analysis method that can eliminate background interference and provide multi-detection modes has strong attraction and value.
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