Bacillus subtilis transformation was conducted in the presence of dimethylsulfoxide and polyethyleneglycol. B. subtilis transformation was most frequent under the effect of 0.1% dimethylsulfoxide and was not altered significantly by polyethyleneglycol. As suggested, an increase of the transformation frequency was associated with the change of the membrane permeability under the influence of dimethylsulfoxide of with the altered activity of the membrane DNase.
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