Neutrophils (PMNs) from patients with secondary iron overload have an increased iron and ferritin content as well as a phagocytosis defect. Several serum components might be incriminated in the cellular iron accumulation. We therefore compared the effects on the PMN phagocytosis of total serum as well as the ferritin and transferrin fractions of serum derived from patients with thalassemia major and healthy control subjects. An incubation system of PMNs was developed. PMN phagocytosis was measured before and after incubation. Total serum from patients with thalassemia induced a defect that was prevented by co-incubation with deferoxamine (DFO). Gel-filtration chromatography was performed to separate the serum fraction containing transferrin and albumin from that containing ferritin. The transferrin-albumin fraction had no effect on PMN phagocytosis. On the contrary, the ferritin fraction of normal serum was deleterious to PMN phagocytosis, and the same fraction from thalassemic serum decreased PMN phagocytosis even more. Co-incubation with DFO or catalase improved this defect. Moreover, a cellular increase in the L-type subunit of ferritin was observed after the incubation of PMNs with the ferritin-containing fraction from thalassemic serum. In conclusion, serum from patients with thalassemia is toxic to PMNs, and this toxicity is due to ferritin-associated iron.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0022-2143(99)90066-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
KSRP Deficiency Attenuates the Course of Pulmonary Aspergillosis and Is Associated with the Elevated Pathogen-Killing Activity of Innate Myeloid Immune Cells.
Cells
December 2024
Department of Dermatology, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
The mRNA-binding protein KSRP (KH-type splicing regulatory protein) is known to modulate immune cell functions post-transcriptionally, e.g., by reducing the mRNA stability of cytokines.
View Article and Find Full Text PDFThe Role of Neutrophils in Allergic Disease.
Clin Exp Immunol
December 2024
Discipline of Paediatrics, School of Medicine, Trinity College Dublin, Ireland.
Neutrophils are short-lived cells of the innate immune system and represent 50-70% of the circulating leucocytes. Their primary role is antimicrobial defence which they accomplish through rapid migration to sites of inflammation followed by phagocytosis, degranulation and the release of neutrophil extracellular traps (NETosis). While previously considered terminally-differentiated cells, they have been shown to have great adaptability and to play a role in conditions ranging from cancer to autoimmunity.
View Article and Find Full Text PDFImpact of a reduced metabolizable protein supply during the transition period on the immune and metabolic status of dairy cows.
J Dairy Sci
December 2024
Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada J1M 0C8. Electronic address:
During the transition period, the incidence of diseases increases due to a negative energy balance that affects metabolic and immune status. Limiting milk production at the beginning of lactation by milking once a day or by incompletely milking twice a day improves the metabolic and immune status of cows. Previous studies have shown that milk production is highly responsive to additional MP.
View Article and Find Full Text PDFHigh-dimensional spectral flow cytometry of activation and phagocytosis by peripheral human polymorphonuclear leukocytes.
bioRxiv
December 2024
Department of Microbiology, Immunology, and Cancer Biology, University of Virginia School of Medicine, Charlottesville, VA, USA.
Polymorphonuclear lymphocytes (PMNs) are terminally differentiated phagocytes with pivotal roles in infection, inflammation, tissue injury, and resolution. PMNs can display a breadth of responses to diverse endogenous and exogenous stimuli, making understanding of these innate immune responders vital yet challenging to achieve. Here, we report a 22-color spectral flow cytometry panel to profile primary human PMNs on population and single cell levels for surface marker expression of activation, degranulation, phagocytosis, migration, chemotaxis, and interaction with fluorescently labeled cargo.
View Article and Find Full Text PDFEffect of type of anticoagulant, transportation time, and glucose in the culture media on neutrophil viability and function test results in dairy cattle.
PLoS One
October 2024
Faculty of Veterinary Medicine, Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Ghent, Belgium.
Article Synopsis
- The study investigates how various factors affect the immune function of dairy cattle's white blood cells (PMN), especially their ability to engulf pathogens (phagocytosis) and produce reactive oxygen species (oxidative burst) using blood samples from Holstein heifers.
- Blood samples were collected in tubes with different anticoagulants (ACD vs. EDTA) and stored at a cool temperature for varying lengths of time to analyze PMN viability and functionality.
- Results showed that while PMN viability dropped significantly after 6 hours of storage for both anticoagulant types, there was no notable difference in PMN function (phagocytosis and oxidative burst) between the two anticoagulants or across the different storage times. *
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!