AI Article Synopsis
- DNA curvature is crucial for various biological processes and was studied by comparing the migration patterns of 11 specially designed oligonucleotides on polyacrylamide gels.
- Temperature significantly impacts the anomalous migration of these sequences, with lower temperatures promoting curvature, while at higher temperatures only one sequence showed anomalous migration.
- The presence of ions like Mg2+ and spermine influences the migration behavior, and sequences with a GGC motif showed unexpected curvature, highlighting their sensitivity to environmental factors, which is important for understanding gene regulation.
Article Abstract
DNA curvature plays an important role in many biological processes. To study environmental influences on DNA curvature we compared the anomalous migration on polyacrylamide gels of ligation ladders of 11 specifically-designed oligonucleotides. At low temperatures (25 degrees C and below) most of the sequences exhibited a degree of anomalous migration. Increased temperature had a significant effect on the anomalous migration (curvature) of some sequences but limited effects on others; at 50 degrees C only 1 sequence migrated anomalously. Mg2+ had a strong influence on the migration of certain sequences, whilst spermine enhanced the anomalous migration of a different set of sequences. Sequences with a GGC motif exhibited greater curvature than predicted by the presently-used angles for the nearest-neighbour wedge model and are especially sensitive to Mg2+. The data have implications for models for DNA curvature and for environmentally-sensitive DNA conformations in the regulation of gene expression.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/07391102.1999.10508294 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Study on the developmental, behavioral toxicity, and toxicological mechanism of the antidepressant drug venlafaxine and its active metabolites in zebrafish.
Environ Toxicol Chem
January 2025
School of Environment and Energy, South China University of Technology, Guangzhou, PR China.
As a representative agent of bicyclic antidepressants, venlafaxine (VEN) has become widely used worldwide and is frequently detected in surface waters with concentrations ranging from ng/L to µg/L. To evaluate the toxicological effects of such medications on aquatic species, studies on environmentally relevant concentrations are essential. Zebrafish were used as a model organism to assess growth and development in larvae and examine tissue accumulation, oxidative stress, and DNA methylation in adults.
View Article and Find Full Text PDFMimicking Nature: Effect of Architectural Design Inspired by Cancellous Bone on the Biological Response of hMSC Cultured on Titanium Scaffolds Fabricated by Laser Beam Powder Bed Fusion.
J Biomed Mater Res A
January 2025
Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland.
Bone tissue regeneration can be affected by various architectonical features of 3D porous scaffold, for example, pore size and shape, strut size, curvature, or porosity. However, the design of additively manufactured structures studied so far was based on uniform geometrical figures and unit cell structures, which often do not resemble the natural architecture of cancellous bone. Therefore, the aim of this study was to investigate the effect of architectonical features of additively manufactured (aka 3D printed) titanium scaffolds designed based on microtomographic scans of fragments of human femurs of individuals of different ages on in vitro response of human bone-derived mesenchymal stem cells (hMSC).
View Article and Find Full Text PDFAssessing molecular changes underlying isopropylated phenyl phosphate (IPP)-induced larval sensorimotor response deficits in zebrafish.
Ecotoxicol Environ Saf
December 2024
Department of Biological Sciences, Clemson University, Clemson, SC, USA. Electronic address:
Article Synopsis
- Isopropylated phenyl phosphates (IPP) are organophosphate flame retardants used in various products, but their leaching raises toxicity concerns due to limited toxicological studies.
- Using zebrafish embryos, the study found significant biological disruptions, including morphological changes and alterations in dopamine levels, alongside behavioral deficits at low concentrations.
- Further analysis indicated IPP inhibits retinoic acid receptor activity and caused hypermethylation in embryos, with distinct impacts observed in the eyes, revealing changes in genes related to nervous system functions.
DNA-Assisted Assays for Studying Lipid Transfer Between Membranes.
Methods Mol Biol
December 2024
State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Frontiers Science Center for Cell Responses, Nankai University, Tianjin, China.
Extended-synaptotagmins (E-Syts) are proteins located on the endoplasmic reticulum (ER) that tether the ER to the plasma membrane (PM) and regulate their lipid homeostasis via its lipid transfer module, the synaptotagmin-like mitochondrial lipid-binding protein (SMP) domain. Here, we describe in vitro DNA nanostructure-assisted lipid transfer assays investigating how the SMP domain transports lipids between membranes and associates with the membranes to extract and release lipids. The lipid transfer signal was detected through fluorescence resonance energy transfer (FRET).
View Article and Find Full Text PDFBmgsb is involved in the determination of cell fate by affecting the cell cycle genes in the silk gland of Bombyx mori.
Int J Biol Macromol
December 2024
State Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, China. Electronic address:
Silk gland is the only organ of silkworm that can produce silk protein, which is a natural macromolecular protein complex and widely utilized in various fields such as biomaterials and biomedicine. The development of silk gland and the expression patterns of silk protein crucial for the silk industry. In this study, the function of a transcription factor Bmgsb was investigated with CRISPR/Cas9 and transgenic system.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!