AI Article Synopsis
- The study investigates how different levels of acidity affect the healing processes of rabbit esophageal cells, as acidity can cause mucosal damage.
- Results show that while isolated cells can tolerate some acidity (pH > 2 for 15 min), their ability to proliferate and heal decreases significantly with lower pH levels and longer exposure times.
- These findings suggest that even minor acid exposure can impair the wound repair mechanisms of esophageal cells, highlighting the importance of acid suppression in treating conditions like acid-related reflux esophagitis.
Article Abstract
Background And Aims: Exposure to luminal acid induces mucosal damage in the oesophagus. The extent and degree of interaction of acid with wound repair mechanisms of the oesophagus have not been explored.
Methods: Rabbit oesophageal cells were isolated, cultured and exposed to different extracellular pHs for several periods of time. Cell mortality was studied in both isolated and primary cultures of these cells by cell flow cytometry using fluorochrome propidium iodide and LDH release. Subconfluent cell cultures were used to study proliferative responses determined by [3H]-thymidine incorporation into DNA. Restitution was studied in confluent monolayers wounded by mechanical denudation.
Results: Isolated cells were resistant to acid when exposed to pH > 2 for 15 min. Cell proliferation increased with small pH decreases (7-6.5) if the period of exposure was less than 6 h, but was inhibited with lower pHs and/or longer periods of acid exposure. However, restitution was gradually inhibited with further pH decreases (6.5-3) and time of acid exposure (0.5-24 h).
Conclusions: The wound repair activities of oesophageal epithelial cells are deeply affected by even small decreases in pH. These findings may help to explain the need for profound acid inhibition in acid-related reflux oesophagitis.
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| http://dx.doi.org/10.1046/j.1365-2036.1999.00491.x | DOI Listing |
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