Supplementing cytophaga agar and broth with 0.5 g l-1 each of D(+) galactose, D(+) glucose, L-rhamnose and skimmed milk gave a dramatic improvement in the isolation of the fish pathogen Flavobacterium psychrophilum. By means of spread-plating, approximately double the number of colonies of larger size were obtained on the improved medium compared to cytophaga agar alone. In supplemented cytophaga broth, growth of Fl. psychrophilum was more rapid and generated greater biomass.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1046/j.1365-2672.1999.00522.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Methylation of foreign DNA overcomes the restriction barrier of and allows efficient genetic manipulation.
Appl Environ Microbiol
January 2025
Department of Biological Sciences, Minnesota State University Mankato, Mankato, Minnesota, USA.
Unlabelled: causes bacterial cold-water disease (BCWD) in salmonids and other fish, resulting in substantial economic losses in aquaculture worldwide. The mechanisms uses to cause disease are poorly understood. Despite considerable effort, most strains of have resisted attempts at genetic manipulation.
View Article and Find Full Text PDFAntibacterial Activity and Action Mechanism of Synthetic Interleukin-8 Derived Peptides Against Flavobacterium psychrophilum.
J Fish Dis
December 2024
Laboratorio de Cultivo de Peces, Departamento de Acuicultura, Universidad Católica del Norte, Coquimbo, Chile.
In Chile, Atlantic salmon and rainbow trout face significant production challenges due to the presence of Flavobacterium psychrophilum, which generates severe disease issues and economic losses. To address this, the salmon industry relies on vaccines and antibiotics, the latter raising concerns about bacterial resistance. For that reason, our study explores an alternative strategy for controlling F.
View Article and Find Full Text PDFInter-Laboratory Comparison of qPCR Assays for Piscirickettsia salmonis in Atlantic Salmon (Salmo salar L.) in 11 Chilean Laboratories.
J Fish Dis
February 2025
Escuela de Medicina Veterinaria, Facultad de Agronomía e Ingeniería Forestal, Facultad de Ciencias Biológicas, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
Real-time PCR (qPCR) testing is an essential component of early detection surveillance systems for Piscirickettsia salmonis infection in Atlantic salmon farms in Chile. Currently, all 11 laboratories in the authorised diagnostic laboratory network use assays based on published protocols. Compared with other P.
View Article and Find Full Text PDFWhole-genome-based taxonomy as the most accurate approach to identify Flavobacterium species.
FEMS Microbiol Lett
January 2024
Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec City, QC G1V 0A6, Canada.
The genus Flavobacterium comprises a diversity of species, including fish pathogens. Multiple techniques have been used to identify isolates of this genus, such as phenotyping, polymerase chain reaction genotyping, and in silico whole-genome taxonomy. In this study, we demonstrate that whole-genome-based taxonomy, using average nucleotide identity and molecular phylogeny, is the most accurate approach for Flavobacterium species.
View Article and Find Full Text PDFAdvancements in rapid diagnostics and genotyping of using Loop-mediated Isothermal Amplification.
Front Microbiol
September 2024
Interdisciplinary Center for Aquaculture Research (INCAR), Universidad de Concepción, Concepción, Chile.
Article Synopsis
- Piscirickettsiosis, caused by the pathogen P. salmonis, significantly impacts the Chilean aquaculture industry, leading to major economic losses, and traditional genotyping methods are limited in cost and expertise requirements.
- This study introduces Loop-mediated Isothermal Amplification (LAMP) as a faster, cheaper, and simpler alternative for diagnosing P. salmonis, developing specific assays for both species identification and differentiation between its genogroups LF-89 and EM-90.
- LAMP assays showed sensitivity and specificity on par with traditional methods while being more suitable for field use, indicating potential for improved disease management and surveillance in salmonid aquaculture.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!