The invention of the polymerase chain reaction (PCR) technique for nucleic acid amplification has had a major impact on many diverse areas of both basic and clinical research. Since its inception in 1985, reports on a wide variety of applications for PCR have received much attention in scientific and medical literature. This technology has been shown to have wide applicability to the diagnosis of human disease, including such diverse areas as infectious diseases, genetic disorders, and cancer. This article presents a broad overview of the principles of PCR including generic concerns that must be addressed when using or designing PCR-based assays. The application of PCR-based assays for the diagnosis of genetic disorders and for infectious disease testing is also discussed.
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Analysis and identification of potential biomarkers for dysfunctional uterine bleeding.
J Reprod Immunol
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Department of Chinese Medicine Rehabilitation, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 50001, China. Electronic address:
Clinical evidence increasingly suggests that traditional treatments for dysfunctional uterine bleeding (DUB) have limited success. In this study, blood samples from 10 DUB patients and 10 healthy controls were collected for transcriptome sequencing. Then, the differentially expressed genes (DEGs) were screened and crossed with the DUB-related module genes to obtain the target genes.
View Article and Find Full Text PDFClinical diagnostic value and potential regulatory mechanisms of lncRNA NOP14-AS1 in chronic kidney disease.
Nucleosides Nucleotides Nucleic Acids
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Urology & Nephrology Center, Department of Nephrology, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, China.
In the early stages, chronic kidney disease (CKD) can be asymptomatic, marking diagnosis difficult. This study aimed to investigate the diagnostic role and potential regulatory mechanisms of nucleolar protein 14 (NOP14) -antisense RNA 1 (AS1) in patients with CKD. Herein, 68 patients with CKD, 65 patients with CKD undergoing peridialysis, and 80 healthy adults were included.
View Article and Find Full Text PDFCell-free DNA release following psychosocial and physical stress in women and men.
Transl Psychiatry
January 2025
Department of Genetic Psychology, Faculty of Psychology, Ruhr-University Bochum, Universitätsstraße 150, Bochum, Germany.
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View Article and Find Full Text PDF[Exploration of oral and pharyngeal swabs in microsatellite instability detection].
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Department of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, China; Heilongjiang Provincial Key Laboratory of Critical Care Medicine, Harbin 150001, China; Central Laboratory of The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China. Electronic address:
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