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Ribosome engineering of Myxococcus xanthus for enhancing the heterologous production of epothilones.
Microb Cell Fact
December 2024
State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao, 266237, People's Republic of China.
Background: Ribosome engineering is a semi-empirical technique used to select antibiotic-resistant mutants that exhibit altered secondary metabolism. This method has been demonstrated to effectively select mutants with enhanced synthesis of natural products in many bacterial species, including actinomycetes. Myxobacteria are recognized as fascinating producers of natural active products.
View Article and Find Full Text PDFChanges in the epidemiology of neonatal bacteremia during the COVID-19 pandemic in Wuhan, China.
Eur J Clin Microbiol Infect Dis
January 2025
Department of Laboratory Medicine, Tongji Medical College, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital, Huazhong University of Science & Technology, Wuhan, 430015, People's Republic of China.
Objective: To investigate the incidence, pathogen distribution, and antibiotic susceptibility of early- and late-onset neonatal bacteremia, and to analyze pathogen trends before and during the COVID-19 pandemic.
Methods: Between January 2016 to December 2022, we collected 879 blood and cerebrospinal fluid specimens from newborns with bacteremia. Bacterial identification used biochemical methods and MALDI-TOF, and antibiotic susceptibility was tested with the VITEK 2 system.
The CRISPR-dCas9 interference system suppresses inhA gene expression in Mycobacterium smegmatis.
Sci Rep
October 2024
Center of Excellence for Innovative Diagnosis of Antimicrobial Resistance, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
CRISPR-dead Cas9 interference (CRISPRi) has become a valuable tool for precise gene regulation. In this study, CRISPRi was designed to target the inhA gene of Mycobacterium smegmatis (Msm), a gene necessary for mycolic acid synthesis. Our findings revealed that sgRNA2 induced with 100 ng/ml aTc achieved over 90% downregulation of inhA gene expression and inhibited bacterial viability by approximately 1,000-fold.
View Article and Find Full Text PDFTranscription Attenuation in Synthetic Promoters in Nonoverlapping Tandem Formation.
Biochemistry
August 2024
Faculty of Medicine and Health Technology, Tampere University, 33520 Tampere, Finland.
Closely spaced promoters are ubiquitous in prokaryotic and eukaryotic genomes. How their structure and dynamics relate remains unclear, particularly for tandem formations. To study their transcriptional interference, we engineered two pairs and one trio of synthetic promoters in nonoverlapping, tandem formation, in single-copy plasmids transformed into cells.
View Article and Find Full Text PDFCRISPR Interference-Mediated Silencing of the Gene in and Its Impact on Antimicrobial Susceptibility.
Antibiotics (Basel)
May 2024
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Background: The discovery of novel therapeutic agents, especially those targeting mycobacterial membrane protein large 3 (), has shown promise. In this study, the CRISPR interference- nuclease-deactivated Cas9 (CRISPRi-dCas9) system was utilized to suppress expression in , and its impacts on susceptibility to antimicrobial agents were evaluated.
Methods: The repression of the gene was confirmed by RT-qPCR.
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