The fluorescence probe [1-(4-trimethylammonium]-6-phenyl-1,3,5-hexatriene (TMA-DPH) displays properties relevant for both monitoring endocytosis kinetics and assessing membrane fluidity by fluorescence-anisotropy measurements (1). Thus, it is, possible with this probe to follow the evolution of membrane fluidity during endocytosis, from the very beginning of the process, i.e., the formation of endocytic vesicles. In most cases, endocytosis is known to start with clathrin-coated vesicles. Still, there are more and more arguments in favor of a complementary endocytic pathway without clathrin. In this article we present membrane-fluidity data for very early endocytosis, which allow an upper limit to be determined for the contribution of a putative nonclathrin pathway. We show that this limit is markedly higher for bone marrow-derived macrophages than for mouse fibroblasts of the L929 cell line.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF02737883 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Efficient degradation of corn straw at low temperature using a novel co-cultured consortium LHWA.
J Biosci Bioeng
January 2025
College of Biological Engineering, Qingdao University of Science and Technology, Qingdao 266045, China.
Article Synopsis
- Straw degradation is slow in cold environments, but a consortium of bacteria and fungi, named LHWA, was developed to enhance this process.
- Under 4 °C, this consortium achieved a 55.52% straw weight loss in liquid fermentation after 30 days and 58.36% in solid fermentation after 60 days.
- Transcriptomic analysis indicated that B. cereus, part of the consortium, enhances cold resistance by modifying cell membrane fluidity and increasing cold stress response proteins.
Tetrabromobisphenol A, but not bisphenol A, disrupts plasma membrane homeostasis in myeloid cell models - A novel threat from an established persistent organic pollutant.
Sci Total Environ
January 2025
Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland; Laboratory of Transcriptional Regulation, Institute of Medical Biology PAS, Lodz, Poland. Electronic address:
Article Synopsis
- The study investigates the effects of Bisphenol A (BPA) and Tetrabromobisphenol A (TBBPA) on the dynamics of biological membranes, focusing on how these persistent organic pollutants impact myeloid cell lines.
- It was found that TBBPA specifically disrupts the plasma membrane's biophysical homeostasis, increasing mobility and decreasing order, while BPA showed no significant effects.
- The findings highlight TBBPA's potential to impair immune function, emphasizing the environmental toxicity concerns associated with persistent organic pollutants.
Linoleic acid alleviates aluminum toxicity by modulating fatty acid composition and redox homeostasis in wheat (Triticum aestivum) seedlings.
J Hazard Mater
January 2025
MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address:
Lipids, as key components of biological membranes, play vital roles in sensing and initiating plant responses to various abiotic stresses. Here, the alteration of membrane fatty acids in wheat roots under Al stress was investigated using two genotypes differing in Al tolerance, and the role of linoleic acid in Al tolerance was comprehensively explored. Significant differences in the fatty acid profiles were observed, with increased linoleic acid accumulation in the Al-tolerant genotype.
View Article and Find Full Text PDFMolecular Mechanisms Linking Omega-3 Fatty Acids and the Gut-Brain Axis.
Molecules
December 2024
Department of Dietetics, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159C, 02-776 Warsaw, Poland.
The gut-brain axis (GBA) is a complex communication network connecting the gastrointestinal tract (GIT) and the central nervous system (CNS) through neuronal, endocrine, metabolic, and immune pathways. Omega-3 (n-3) fatty acids, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are crucial food components that may modulate the function of this axis through molecular mechanisms. Derived mainly from marine sources, these long-chain polyunsaturated fatty acids are integral to cell membrane structure, enhancing fluidity and influencing neurotransmitter function and signal transduction.
View Article and Find Full Text PDFLTX-315 is a novel broad-spectrum antimicrobial peptide against clinical multidrug-resistant bacteria.
J Adv Res
January 2025
State Key Laboratory of Chemical Biology and Drug Discovery and the Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong; Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, The Hong Kong PolyU Shenzhen Research Institute, Shenzhen, PR China. Electronic address:
Introduction: Infections stemming from multidrug-resistant bacteria present a substantial threat to public health today. Discovering or synthesizing novel compounds is crucial to alleviate this pressing situation.
Objective: The main purpose of this study is to verify the antibacterial activity of LTX-315 and explore its primary action mode.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!