A biotin-binding RNA pseudoknot developed through in vitro selection has been crystallized using the hanging-drop vapor-phase diffusion method. The X-ray diffraction data indicate that the crystals belong to the space group P4222 with unit-cell parameters a = b = 55.2, c = 62.7 A and alpha = beta = gamma = 90 degrees. The crystals are 120 x 80 x 40 microm and VM = 2.17 A3 Da-1. The crystals diffract to 2.8 A.
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