A modified fast micro method for spectrotypic/clonotypic analysis of human IgG1-4 antibodies against bacterial virulence antigens of polysaccharide or protein nature is described. Serum samples of as small volumes as 0.5 microliter were isoelectrically focused in micro agarose gels made in plexiglass matrices and blotted using immunoaffinity-mediated capillary blotting onto nitrocellulose membranes previously coated with antigen. The bands of the antigen-specific antibodies were identified with respect to isotypes, light chain types, allotypes or idiotypes by incubating the nitrocellulose membranes with mouse monoclonal anti-human IgG subclass antisera and then with alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulins. The method was applied for characterization of human monoclonals against tetanus toxoid (TT) and for the analysis of variable patterns of clonotypes in IgG subclass-deficient patients. The usefulness of the technique was also demonstrated by comparing the variable specificity and reactivity of different commercial monoclonals against human IgG subclasses. This method is fast, specific, sensitive, uses little material, is simple and reproducible.
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J Chem Inf Model
January 2025
Theory and Simulation of Complex Systems, Institute of Physical Chemistry, Heinrich-Heine Universität, Universitätsstr. 1, 40225 Düsseldorf, Germany.
Understanding and analyzing large-scale reaction networks is a fundamental challenge due to their complexity and size, often beyond human comprehension. In this paper, we introduce AUTOGRAPH, the first web-based tool designed for the interactive three-dimensional (3D) visualization and construction of reaction networks. AUTOGRAPH emphasizes ease of use, allowing users to intuitively build, modify, and explore individual reaction networks in real time.
View Article and Find Full Text PDFHeliyon
January 2025
Advanced Materials Research Laboratory, Department of Physics, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia.
Herein, we report the biosynthesis of pure NiO and NiO nanoparticles doped with Silver (Ag@NiO NPs) 2, 4, 6, and 8 mol% from aloe vera extract by solution combustion method at 400 °C and calcined at 500 °C for 3 h. By utilizing silver-doped NiO nanoparticles synthesized with Aloe Vera latex, which not only enhances the material's properties but also promotes environmentally friendly fabrication methods. The morphological, structural elemental compositions were analysed through SEM, HRTEM, SAED, XRD and EDAX.
View Article and Find Full Text PDFJ Hazard Mater
January 2025
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China. Electronic address:
A series of novel cationic modified actinia-shaped composite coagulant (AMS-C), with similar tentacle length and distribution but different charge density (CD), was successfully designed and fabricated by combination of a cationic graft starch and attapulgite (ATP). AMS-C shows a high efficiency in coagulative removal of Microcystis aeruginosa from water over a wide pH range. The algae-harvesting efficiency of optimized AMS-C can reach to 92.
View Article and Find Full Text PDFNutr Metab Cardiovasc Dis
December 2024
Division of Cardiology, Department of Clinical and Molecular Medicine, University of Rome Sapienza, Sant'Andrea Hospital, Rome, Italy. Electronic address:
Background And Aims: Obesity represents a crucial modifiable risk factor for cardiovascular complications. Two dietary approaches, Very Low-Calorie Ketogenic (VLCKD) and Intermittent Fasting (IFD) diets, have demonstrated to reduce blood pressure (BP) and produce cardiovascular and metabolic advantages. We aimed to evaluate the effects of VLCKD or IFD compared to Free Diet (FD) on office brachial and central systolic BP levels.
View Article and Find Full Text PDFMethods Appl Fluoresc
January 2025
Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, No.36 Sanhao Street, Heping District, Shenyang, Liaoning Province, China, Shenyang, 110004, CHINA.
Molybdenum disulfide quantum dots (MoS2 QDs) is a new type of graphite like nanomaterial, which exhibited well chemical stability, unique fluorescence characteristics, and excellent biocompatibility. The conventional hydrothermal synthesis of MoS2 generally requires a long-term reaction at high temperature and high pressure. Herein, we have developed a simple and fast MoS2 QDs synthesis scheme using microwave heating, and further modified the surface of MoS2 QDs using 3-aminophenylboronic acid.
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