Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1103/PhysRevLett.66.576 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Increased proportions of invasive pneumococcal disease cases amongs adults experiencing homelessness sets stage for new serotype 4 capsular-switch recombinant.
J Infect Dis
September 2024
Centers for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases, Division of Bacterial Diseases, Respiratory Diseases Branch, Atlanta, GA, USA.
Article Synopsis
- The CDC's research revealed a rise in serotype 4 invasive pneumococcal disease (IPD) cases, especially among adults experiencing homelessness between 2016-2022.
- An analysis of IPD isolates identified a new serotype 4 variant created through genetic recombination between two parental strains, occurring mainly in Oregon.
- The increase in serotype 4 cases, highlighting transmission among adults living in homelessness, may indicate a concerning trend in bacterial evolution and disease spread.
Two multi-fragment recombination events resulted in the β-lactam-resistant serotype 11A-ST6521 related to Spain9V-ST156 pneumococcal clone spreading in south-western Europe, 2008 to 2016.
Euro Surveill
April 2020
Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de LLobregat, Spain.
BackgroundThe successful pneumococcal clone Spain9V-ST156 (PMEN3) is usually associated with vaccine serotypes 9V and 14.AimOur objective was to analyse the increase of a serotype 11A variant of PMEN3 as cause of invasive pneumococcal disease (IPD) in Spain and its spread in south-western Europe.MethodsWe conducted a prospective multicentre study of adult IPD in Spain (2008-16).
View Article and Find Full Text PDFCRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in .
J Biol Eng
November 2019
LOEWE Center for Synthetic Microbiology, Philipps-University Marburg, Hans-Meerwein Str. 6, 35032 Marburg, Germany.
Background: Synthetic biology heavily depends on rapid and simple techniques for DNA engineering, such as Ligase Cycling Reaction (LCR), Gibson assembly and Golden Gate assembly, all of which allow for fast, multi-fragment DNA assembly. A major enhancement of Golden Gate assembly is represented by the Modular Cloning (MoClo) system that allows for simple library propagation and combinatorial construction of genetic circuits from reusable parts. Yet, one limitation of the MoClo system is that all circuits are assembled in low- and medium copy plasmids, while a rapid route to chromosomal integration is lacking.
View Article and Find Full Text PDFDynamics and impact of homologous recombination on the evolution of Legionella pneumophila.
PLoS Genet
June 2017
Pathogen Genomics, Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
Legionella pneumophila is an environmental bacterium and the causative agent of Legionnaires' disease. Previous genomic studies have shown that recombination accounts for a high proportion (>96%) of diversity within several major disease-associated sequence types (STs) of L. pneumophila.
View Article and Find Full Text PDFA system for multilocus chromosomal integration and transformation-free selection marker rescue.
FEMS Yeast Res
December 2014
Department of Chemical Engineering, Stanford University, Stanford, CA, USA.
Yeast integrating plasmids (YIPs) are a versatile tool for stable integration in Saccharomyces cerevisiae. However, current YIP systems necessitate time- and labor-intensive methods for cloning and selection marker rescue. Here, we describe the design, construction, and validation of a new YIP system capable of accelerating the stable integration of multiple expression constructs into different loci in the yeast S.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!