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Morphological and Phylogenetic Analyses of Ceratomyxa tessaloniensis n. sp. In Astyanax mexicanus De Fillipi, 1853 (Characiformes: Characidae) from the Flexal River, Tessalônica Community, Amapá, Amazon, Brazil.
Acta Parasitol
January 2025
Laboratory of Morpho-Molecular Integration and Technologies, Federal Rural University of the Amazon (UFRA), Belém, State of Pará, Brazil.
Purpose: This work described a new species of Ceratomyxa, based on morphological and phylogenetic analyzes of myxospores collected from the gallbladder of the fish Astyanax mexicanus.
Methods: Sixty-two specimens were captured, between December 2022 and February 2024, in the Flexal River, in the community of Tessalônica, state of Amapá. The specimens were transported alive to the Laboratory of Morphophysiology and Animal Health, at the State University of Amapá, where the studies were carried out.
Obstacles in quantifying A-to-I RNA editing by Sanger sequencing.
Methods Enzymol
January 2025
Faculty of Biology, Technion - Israel Institute of Technology, Technion City, Haifa, Israel. Electronic address:
Adenosine-to-Inosine (A-to-I) RNA editing is the most prevalent type of RNA editing, in which adenosine within a completely or largely double-stranded RNA (dsRNA) is converted to inosine by deamination. RNA editing was shown to be involved in many neurological diseases and cancer; therefore, detection of A-to-I RNA editing and quantitation of editing levels are necessary for both basic and clinical biomedical research. While high-throughput sequencing (HTS) is widely used for global detection of editing events, Sanger sequencing is the method of choice for precise characterization of editing site clusters (hyper-editing) and for comparing levels of editing at a particular site under different environmental conditions, developmental stages, genetic backgrounds, or disease states.
View Article and Find Full Text PDFPrenol production in a microbial host via the "Repass" Pathways.
Metab Eng
January 2025
Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, USA. Electronic address:
Prenol and isoprenol are promising advanced biofuels and serve as biosynthetic precursors for pharmaceuticals, fragrances, and other industrially relevant compounds. Despite engineering improvements that circumvent intermediate cytotoxicity and lower energy barriers, achieving high titer 'mevalonate (MVA)-derived' prenol has remained elusive. Difficulty in selective prenol production stems from the necessary isomerization of isopentenyl diphosphate (IPP) to dimethylallyl diphosphate (DMAPP) as well as the intrinsic toxicity of these diphosphate precursors.
View Article and Find Full Text PDFsp. nov., isolated from tree bark ( Chev.) and its antioxidant activity.
Int J Syst Evol Microbiol
January 2025
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
A Gram-stain-positive, facultatively anaerobic, rod-shaped strain, designated SPB1-3, was isolated from tree bark. This strain exhibited heterofermentative production of dl-lactic acid from glucose. Optimal growth was observed at 25-40 °C, pH 4.
View Article and Find Full Text PDFPost-transcriptional regulation of aromatic amino acid metabolism by GcvB small RNA in .
Microbiol Spectr
January 2025
Department of Infection Biology, Institute of Medicine, University of Tsukuba, Ibaraki, Japan.
synthesizes aromatic amino acids (AAAs) through the common pathway to produce the precursor, chorismate, and the three terminal pathways to convert chorismate into Phe, Tyr, and Trp. also imports exogenous AAAs through five transporters. GcvB small RNA post-transcriptionally regulates more than 50 genes involved in amino acid uptake and biosynthesis in , but the full extent of GcvB regulon is still underestimated.
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