Influence of pregnancy on iron and copper exchange rates in women with normal body weight and women with obesity.

The number of obese pregnant women increases annually and reaches 20-30%. The metabolism of hormones and minerals changes in the presence of a large amount of adipose tissue in the body of a pregnant woman, which leads to a number of obstetric and perinatal problems. The aim of the work is to study and compare the influence of the gestational process on the indicators of iron and copper metabolism in the blood serum of women with normal body weight and women with obesity.

[The investigation of alpha1-antitripsin, orozomukoid and ceruloplasmin in peripheral blood and oral fluid. Paradontitis is one of more risk factors of cardiovascular diseases].

This article presence the connection between paradontitis and cardiovascular diseases, and definition of the maintenance of acute phase proteins in an oral fluid at patients with acute myocardial infarction is obviously important for clinic. Results of own researches of change of the maintenance of three acute phase proteins: ceruloplasmin, alpha1-antitripsin and orosomucoid in an oral fluid and blood plasma at paradontitis and myocardial infarction allow to consider the paradontitis as one more risk factor of a cardiovascular pathology, except well-known hypertensions, smoking, a diabetes.

[The influence of acute alcohol intoxication on glycogen content in the liver and sceletal muscles].

The influence of ethanol on glycogen value in liver and skeletal muscle was investigated at rats and forensic cases. In 24 hours after ethanol intake glycogen value in rat's liver was low. By autopsy examinations liver glycogen value was low in cases as acute ethanol poisoning as coronary heard diseases with alcohol condition.

[Diagnostic value of the determination of salivary acute-phase proteins in patients with myocardial infarction].

The salivary and plasma levels of acute phase proteins were determined in 59 patients with myocardial infarction (MI). In acute and subacute MI, the content of ceruloplasmin, orosomucoid, and alpha1-antitrypsin increased not only in the plasma, but also in the saliva. A noninvasive procedure was devised to diagnose the termination of an inflammatory process in the necrotic process in MI, which implies the determination of salivary ceruloplasmin, if the latter is less than 106 mg/l, inflammation completeness in the necrotic area is diagnosed.

[A new view on pathochemical mechanisms of prolonged peritoneal dialysis].

New data on etiology, pathogenesis, clinics, quantity estimation, treatment and complications of peritoneal dialysis are observed. The role of aquaporine, nitric oxide, NO-synthase, inflammation and sepsis markers (procalcitonine, C-reactive protein) in pathochemical mechanism of peritoneal dialysis is discussed.

[Experimental rationale for the use of enterosorbents in acute ethanol intoxication].

The experiment on 83 rats has provided a rationale for the use of enterosorbents in acute ethanol intoxication. Polysorb reduces the halflife of ethanol, recovers physical fitness in the animals with acute poisoning. The enterosorbents polysorb, litovit, and sapropel have been found to have a corrective effect on the level of the major plasma antioxidant ceruloplasmin in acute ethanol intoxication.

[Analysis of tear enzymes in the diagnosis of obstructive jaundice associated with subhepatic cholestasis].

The activity of enzymes, such as alkaline phosphatase, gamma-glutamyl transpeptidase, and leicine aminopeptidase, was found to be increased in the serum and tear of 28 patients with obstructive jaundice. In malignant tumor-induced jaundice, the activity of the enzymes, the indicators of cholestasis, was higher than that in obstructive jaundice caused by extrahepatic bile duct calculi. A new method has been devised for noninvasive diagnosis of obstructive jaundice from tear enzyme immunoassay.

[Effect of bacteriophage on the serum level of iron and copper in pregnant women with pyelonephritis].

Eighty-nine pregnant women, including 62 pregnant females with pyelonephritis, were examined. After bacteriophage treatment for pyelonephritis, there was an increase in the content of iron, a decrease in the level of soluble transferrin receptor without iron therapy, suggesting iron redistribution in pregnant woman. The blood levels of copper, ceruloplasmin, and transferrin in pregnant women with pyelonephritis point to antioxidative defense activation in both healthy pregnant women and pregnant females with pyelonephritis.

[Pathogenesis of ophthalmoherpes basing on the analysis of enzymes of the eye tissue and fluids].

To specify mechanisms of pathology development, we studied the activity of lysosomal glycosides in rabbit ocular tissues in experimental ophthalmoherpes. Rabbits with herpetic kepatitis show activation of beta-glucuronidase, beta-glycosidase, alpha-mannosidase in cornial epithelium and stroma, iris, aqueous humor of herpes-infected and contralateral eye. The activity of the above three glycosidases in the tears of children with herpetic keratitis was enhanced.

[Antioxidative defense parameters in acute and chronic cholecystitis].

A hundred and fifty-two patients cholelithiasis were examined. Among them, 69 and 83 patients were operated on for acute destructive and chronic cholecystitis, respectively. In destructive cholecystitis, there is a high lipid oxidation rate accompanied by the elevated levels of nonenzymatic antioxidant ceruloplasmin.

[Use of tear enzyme assay to predict recurrent herpetic keratitis in children].

The purpose of the study was to develop a procedure for predicting a relapse of herpetic keratitis in children, by taking into account the results of tear biochemical analysis. The tears from 47 children with herpetic keratitis were examined for the levels of total protein, the concentration of acute-phase proteins, such as orosomucoid and C-reactive protein, the activities of transferases: gamma-glutamyltranspeptidase transferase, aspartate aminotransferase, and alanine aminotransferase, those of lysosomal glycosidases: alpha-mannosidase, beta-glycosidase, and beta-glucuronidase. Tear biochemical assay made it possible to evaluate the efficiency of treatment and to develop a procedure for predicting a recurrence of herpetic keratitis in children.

[Effect of sapropel mud on the parameters of oxidative stress and antioxidative defense in acute carbofos poisoning].

Sapropel was tested for its effect on the permeability of erythrocytic membranes and on the erythrocytic and serum levels of ceruloplasmin, malonic dialdehyde, and dienic conjugates in rats after acute poisoning by carbofos, a malathion insecticide. The increased processes of free radical oxidation during carbofos poisoning were suggested by the higher rates of chemiluminescence of erythrocytes and peripheral blood serum. No significant changes in the parameters of blood oxidative stress in the carbofos-poisoned animals during therapeutic-and-prophylactic use of sapropel indicate that the latter has antioxidative properties.

[Prophylaxis of acute postoperative gastroduodenal ulcers at the patients with colorectal cancer].

Effective method for prophylaxis of acute postoperative gastric ulcers and erosions was developed. The core of this method is ozone therapy in preoperative period. Intensity of blood serum and erythrocytes chemiluminescence and ATPase activity of erythrocytes were studied at 86 patients with colorectal cancer before surgery and at 1st and 4th days of postoperative period.

[Biochemical analysis of the vitreous body of the eye in post-mortem diagnosis of diabetic coma].

The biochemical test of the vitreous body (VB) may be used in post-mortem diagnosis of diabetes mellitus and diabetic coma. Concentrations of glucose, lactate, keton bodies in the VB of the eye do not depend on duration of post-mortem period. Methods of diagnosis of hyperglycemic, hypoglycemic and ketoacidotic comas in the postmortem period are proposed.

[The activity of adenosinetriphosphatase of erythrocytes in peripheral blood of patients with colorectal cancer].

We studied the activity of whole ATPase, Mg2+ -ATPase and Na+, K+ -ATPase of peripheral blood erythrocytes in 68 patients with colorectal cancer before surgery and immediately after it and found such activity to be reduced. The low activity of Na+, K+ -ATPase of peripheral blood erythrocyte was shown as possible for use in prognosticating acute postoperative erosive-ulcerous lesions of the gastric tunic. Ozone therapy, if undertaken preoperatively, restores the ATPase activity.

[Chemiluminescence analysis of biological fluids in patients with diabetes mellitus].

Chemiluminescence analysis (CA) was made for different biological fluids in 50 patients with diabetes mellitus (DM). Intensified chemiluminescence of erythrocytes, blood serum, urine and tear is indicative of an enhanced free-radical oxidation in diabetes mellitus. CA of biological fluids made in DM patients reflects a disease severity.

[Biochemical analysis of children tears in herpetic keratitis].

The processes of free-radical oxidation aggravate and the antioxidant corneal protection worsens in children with herpetic keratitis, which is confirmed by a higher chemiluminescence of the lachrymal fluid and by a lower peroxidase activity in it. The study results are indicative of the feasibility to add the antioxidants to the complex therapy of herpetic keratitis. On the basis of the enzyme assay a method was designed to prognosticate the possibility of relapses of ophthalmoherpes in children.

[A new bone/blood index in the study of labelled citrate metabolism in rat bones in fracture, deafferentation, rickets, and caries].

A new bone/blood index has been developed which is calculated by dividing % of radionuclide inclusion in the bones by % of the inclusion in the serum. Graphic image of the index changes with time is significantly more demonstrative than digital tables and allows one not only easily measure distribution of the labelled compound between fractions diluted in the blood and sorbed on the bone but also specify a major direction of their transport between blood and bone. The index confirms the presence of biphasic primary fast but weak physical adsorption and secondary slow and strong chemisorption.

[Activity of the antioxidant enzymes of erythrocytes in herpetic keratitis].

Free-radical oxidations get activated, the activity of catalase slows down and the activity of superoxiddismutase increases in peripheral blood erythrocytes of patients with herpetic keratitis, which is indicative of a changing antioxidant protection in late stages of herpetic infection. The results reveal new chains in the evolution of ophthalmic herpes and confirm the feasibility of adding antioxidants to the complex therapy of patients with herpetic keratitis.

[Mechanisms of an antioxidative action of erythrocytes in experimental ophthalmoherpes infection].

Experimental ophthalmoherpes infection activates free-radical oxidation in red cells from rabbit and rat peripheral blood, inhibits catalase activity, enhances activity of superoxide dismutase demonstrating changed antioxidative defense at early stages of herpes infection.

[Novel methods in diabetes mellitus diagnosis].

Analysis of the lacrimal fluid was carried out for the diagnosis of diabetes mellitus in 68 patients. Collection of tears is absolutely painless, atraumatic, and involves virtually no risk of viral infections. Analysis of the lacrimal fluid is particularly valuable for repeated analyses over the course of disease.

[Antioxidant enzyme activity in the erythrocytes of rats with alloxan diabetes].

The authors studied changes in chemiluminescence and activity of three antioxidant enzymes in peripheral blood red cells in experimental diabetes mellitus induced in rats by alloxan injection. Intensification of red cell chemiluminescence reflected enhanced lipid peroxidation. High activity of catalase and superoxide dismutase activity was compensatory.

[Glycolytic metabolites and adenosine triphosphoric acid in the herpes-infected eye].

In the tissues of the herpes-infected eye of a rabbit the content of adenosine triphosphoric acid (ATP), glucose and pyroracemic acid decreases and the level of lactic acid rises, i.e. anaerobic glycolysis prevails over the aerobic decomposition of glucose.