[Functional significance of 2 pathways of energy transport in cardiomyocytes].

To study the relative roles of creatine kinase (CK) and adenylate systems in cardiac energy turnover, the effect of CK inhibitor, iodoacetamide- (IAA, 0.5 mM), and 2-deoxyglucose-(DOG, 2 mM) induced) 65% depletion of adenine nucleotides at slightly decreased CK flux was determined in isolated rat heart. Both substances did not substantially affect contractile parameters of the isovolumic heart.

[The electrophoretic typing of rotaviruses in the clinico-epidemiological study of infection].

The electrophoretic study of the RNA of human rotavirus strains (1146 strains) circulating in 9 cities at the territory of the European part of the RSFSR was carried out. The electrophoretic (EP) types of rotavirus were established in each individual area. The study revealed that the most widespread rotaviruses responsible for the majority of gastroenteritis cases are those with the long EP type of RNA, characterized by the joint migration of segments 2, 3 and 7 (the 3rd EP type of RNA).

[The mechanism of milk contamination in the territory of Gomel Oblast].

The authors provide the results of studying 137Cs and 90Sr pollution of soil, plants and milk in the Gomel region in 1986-1989. Describe the dynamics of the ratios soil-grass and milk-soil during that period. The content of 137Cs in milk ranged from n.

[Role of creatine phosphokinase in the energy supply of the pumping function of the heart].

The cardiac output of isolated working rat heart and left ventricular pressure were estimated in either almost complete inhibition of creatine kinase by iodoacetamide or predominant fall in adenine nucleotides (AdN) content induced by 2-deoxyglucose treatment. In the former case, a profound cardiac pump failure was observed despite almost normal levels of myocardial AdN and phosphocreatine. Those hearts could not maintain the aortic output at standard load due to lower LV systolic pressure, that was accompanied by increased minimal and maximal diastolic pressures by 5-7 mm Hg as well as by LV diastolic stiffness.

[An analysis of the gene coding for the basic neutralizing antigen VP7 of human rotavirus isolate 1407].

A procedure based on polymerase chain reaction was developed for the study of rotaviruses. A full-length cDNA copy of the gene coding for major neutralizing glycoprotein VP7 of a human rotavirus isolate 1407 with the "long" electrophoretype (classified as the first electrophoretype) was cloned and sequenced. The primary structure of glycoprotein VP7 of isolate 1407 in A and C antigenic regions was found to be similar to that of serotype 1 virus.

[The detection of rotavirus RNA in nasopharyngeal smears by molecular hybridization].

Rotavirus RNA was detected in nasopharyngeal washings, obtained from patients with rotavirus gastroenteritis (76.3% of cases) and gastroenteritis of unknown etiology (34.7% of cases), by the method of molecular hybridization with the use of RNA of monkey virus SA11, labeled with 32P in the process of T4-ligase reaction.

[A comparison of the effect of tranquilizers in experiments and clinical practice].

Experimental and clinical study of the analgesic effects of mebicar and benzodiazepine series tranquilizers (sibazon and chlozepid) and their combinations with non-narcotic analgesics (amidopyrin, pyranal) or local anesthetic lidocaine has demonstrated a more marked effect of benzodiazepine derivatives on pain sensitivity thresholds as against mebicar. Tranquilizer combinations with other analgesics helped define the optimal premedication variants: benzodiazepine derivative augment analgesic activity whereas mebicar potentiates lidocaine effect.

[Monoclonal antibodies BCA-09 against blast cell surface antigen in acute myelomonoblastic leukemia].

Data are presented on the study of BCA-09 monoclonal antibodies (MCA) produced by 2G3 hybrid cells (a new strain). The conventional method of somatic hybridization of mouse myeloma cells and splenocytes of mice immunized with peripheral blood leucocytes from a patient with acute myelomonoblastic leukemia (AMML) was used to obtain hybridomas. The study of BCA-09 MCA specificity has shown that in more than half of cases they reveal surface membrane antigen of leukemia cells in patients with AMML, and do not react with blast cells of patients with other variants of leukemia and lymphosarcoma, as well as with blood cells of normal subjects, and with some human cultural cell lines.

[Use of the polymerase chain reaction for analysis of rotaviruses. Nucleotide sequence of a gene, coding for the basic neutralizing antigen VR7 of a human rotavirus with a new G-serotype].

A procedure based on polymerase chain reaction use for the detection of rotavirus has been developed. Full length cDNA copy of the VP7 gene coding for the major neutralization glycoprotein of the human rotavirus RK9 with an unusual "wide" electrophoretype is cloned and sequenced. Glycoprotein VP7 of RK9 has a unique amino acid composition in A and C antigenic regions.

[Blood immunogenetic markers in suppurative-infectious diseases].

The results are available of typing red blood cells (ABO, Rh, MN) and HLA antigens (locus A, B) as well as haptoglobulin in 118 cases of pyosepsis. The occurrence of some HLA-antigens and of haptoglobulin types was specified for an overall group of patients, staphylococcal and pseudomonas sepsis, vital infection patients. Distribution of the above immunogenetic markers in separate nosological variants of pyoseptic infection has been analyzed.

[Study of the optimal conditions of preservation of cephalosporin C-producing fungus Acremonium chrysogenum].

The influence of various preservation conditions of viability and antibiotic activity of Acremonium chrysogenum 281A and 305A strains producing cephalosporin C was studied. Cryogenization of the culture in the form of suspension of the vegetative mycelium in 20 per cent glycerol solution at a rate of 1 degree/min showed to be advantageous over lyophilization and L-drying. Cryogenization under such conditions provided rather high viability of the culture and preservation of its initial antibiotic activity for the period of its storage for at least 1.

[The effect of caffeine on the cardiac diastole and systole in rats and guinea pigs].

The pump function and left ventricular (LV) pressure of isolated rat or guinea pig hearts were measured during caffeine infusion into the perfusate at increasing concentrations (0.1-0.25 mM for rats and 1-5 mM for guinea pigs).

[The synergistic action of glutamic acid and phosphocreatine on the metabolism and function of the heart during ischemia and reperfusion].

The combined effect of glutamic acid (15 mM) and phosphocreatine (10 mM) on metabolism and postischemic recovery of cardiac function was studied in isolated perfused working guinea pig hearts. Addition of these two agents into standard hyperpotassium cardioplegic solution increased twice the recovery of the aortic output and improved restoration of volume work and an index of functional recovery. This effect was combined with the complete recovery of ATP, phosphocreatine, the decrease in ammonia and lactate tissue contents and preservation of amino acid pool.

[Mast cell and blood coagulation functions during prolonged noradrenaline administration].

During the long term noradrenaline injection (0.56 microgram/kg/min-2 hours during six days) the number of connective tissue mast cells decreases in dogs. Mature cells disappears and the percentage of young forms increases.

[Artificial crystalline lens of N. M. Sergienko's design--results of 1000 operations].

The technique of extracapsular cataract extraction with implantation of N. M. Sergienko's posterior-chamber artificial lens is simple and does not involve the use of sophisticated expensive equipment.

[Immunologic variants of acute lymphoblastic leukemia in adults and its correlation with cytochemical and various clinical parameters].

The authors provide data pertaining to the immunological phenotyping in the panel of monoclonal antibodies to various differentiating antigens of the leukemic cells in 27 patients with acute lymphoblastic leukemia (ALL). Based on the composition of the differentiating antigens of the leukemic cells the following immunological variants of the disease were identified: pre-B, pre-T and T-ALL--the thymic and mature (activated) ones. The phenotype was compared with the cytochemical parameters of the lymphoblasts of the identified immunological variants of ALL and with some clinical and hematological manifestations of the disease.

[Electrophoretic analysis of genome RNA of human rotaviruses].

The schemes for profiles of migration of RNA segments from human rotaviruses isolated in Gorky city in 1981-1987 are presented. The groups of the "long", "short" and "wide" electrophoretypes were identified. The dominant rotaviral phoretypes are determined.

[Sulfide ooze mud and sodium chloride baths in treating osteoarthrosis patients].

Humoral immunity initially affected in patients with osteoarthrosis returns to normal under the influence of a multiple-modality treatment involving application of sulphide moor in combination with sodium chloride baths.

Adaptation of cardiac contractile function to conditions of chronic energy deficiency.

Left ventricular (LV) contractile function and pump function were depressed in isolated working hearts from rats treated with either guanidinopropionic acid (GPA), an inhibitor of creatine influx, or the anthracycline antibiotic, adriamycin, for 6 and 10 weeks, respectively. In both groups of treated animals myocardial phosphocreatine content was lower than in control hearts, while ATP content was unchanged. Hearts of treated animals exhibited only a minor depression of cardiac output with a submaximal pressure load or during volume overload.