Intranasal liposomal remdesivir induces SARS-CoV-2 clearance in K18-hACE2 mice and ensures survival.

A huge challenge after the emergence of COVID-19 has been the discovery of effective antiviral drugs. Although remdesivir (RDV) emerged as one of the most promising drugs, its pharmaceutical formulation Veklury® is limited by moderate efficacy, high toxicity and need for parenteral administration. The aim of the present work was to develop a liposomal formulation of RDV for pulmonary administration and evaluate its efficacy in models of COVID-19.

Clinical and laboratory findings of hookworms ( spp.) in naturally infected adult dogs.

Intestinal parasites of the genus are the most prevalent in coproparasitological examinations and necropsies of dogs in Brazil. Although adult dogs often remain asymptomatic when infected, there is limited published information concerning the laboratory and clinical findings and severity of infection in symptomatic adult dogs. Therefore, this study aimed to characterize the clinical and laboratory findings of adult -infected dogs.

Chemical and in vivo analyses of calcium silicate-based materials in bone and connective tissues.

Aim: Calcium silicate-based cements have been widely used in dentistry mainly due to their physicochemical and biological properties. Commercially available materials use radiopacifiers containing metals (bismuth, tantalum, tungsten and/or zirconium). To investigate volumetric changes, in vivo biocompatibility and systemic migration from eight commercially available materials, including powder/liquid and 'ready-to-use' presentations.

Wildfire ashes: evaluating threats on the Pantanal wetland reserve (Mato Grosso, Brazil) using ecotoxicological tests.

In 2020, the largest continuous wetland area on the planet, the Brazilian Pantanal, experienced an unprecedented fire that affected the entire ecosystem. Our goal was to elucidate the effects of ash presence following the fire events. We quantified the impact of ashes, collected in four Conservation Units, on soil, water, and atmosphere.

Clinical, radiographic, and histopathological characterization of Osteomyelitis of the Jaws: A 51-Year experience at an oral pathology service.

Introduction: Osteomyelitis is an inflammatory condition of the bone with a multifactorial etiology, including trauma and odontogenic infections. Despite being a common condition in clinical practice, limited research exists on the clinical and pathological profile of Osteomyelitis of the Jaws (OJ). This 51-year retrospective study aimed to describe the clinical, radiographic, and histopathological features of OJ diagnosed at a single oral pathology referral center.

Association Between Physical Activity Levels and Symptoms of Temporomandibular Disorders in Office Workers: A Cross-Sectional Observational Study.

Background: Sedentary work is a potential health risk factor and may exacerbate painful complaints, while physical activity may serve as a preventive factor for the onset and worsening of musculoskeletal disorders. However, there is scarce evidence on how engaging in leisure physical activity may influence the reporting of temporomandibular dysfunction (TMD) symptoms.

Objective: To assess the association between the level of physical activity and TMD symptoms in office workers.

Qualitative chemical characterization of salva-de-marajó (Lippia origanoides, Verbenaceae) preparations.

Lippia origanoides Kunth (LO, Verbenaceae), commonly known in Brazil as salva-de-marajó, is an aromatic plant native to the Americas. Quilombola women from Oriximiná (Pará State, Brazil) use decoctions and decoction vapors of LO aerial parts orally or in sitz baths to alleviate symptoms of dysmenorrhea. This study aimed to evaluate LO decoctions and essential oils through a chemo-qualitative approach.

Ru(II)-diphosphine/N,S-mercapto complexes and their anti-melanoma properties.

We have synthesized and characterized a novel series of ruthenium complexes with formulas [RuCl(N-S)(dppm)]PF (Ru1), [Ru(N-S)(dppm)]PF (Ru2), [Ru(N-S)(dppe)]PF (Ru3), [Ru(N-S)(dppen)]PF (Ru4), [Ru(N-S)(bpy)]PF (Ru5). In these formulas, N-S or S represents H2mq (2-mercapto-4(3)-quinazoline); dppe (1,2'-bis(diphenylphosphine)ethane), dppm (1,1'-bis(diphenylphosphine)methane), or dppen (1,2'-bis(diphenylphosphine)ethene); and bpy refers to 2,2'-bipyridine. We have also compared the cytotoxicity of cisplatin with these ruthenium complexes to murine melanoma cells (B16-F10), human melanoma cells (A-375), and the non-tumoral human keratinocyte cell line (HaCat).

Resveratrol alters oral biofilm in vitro and in vivo.

Objective: To evaluate the ability of resveratrol to reduce dental caries in vitro and in vivo.

Methods: In part 1, a microcosm biofilm protocol was employed. One hundred twenty-six bovine enamel specimens were treated with: Resveratrol (50, 100, 200, 400 µg/mL), Phosphate buffered saline (negative control), Dimethyl sulfoxide (negative control) and 0.

Engineering peroxisomal surface display for enhanced biosynthesis in the emerging yeast Kluyveromyces marxianus.

The non-conventional yeast Kluyveromyces marxianus is a promising microbial host for industrial biomanufacturing. With the recent development of Cas9-based genome editing systems and other novel synthetic biology tools for K. marxianus, engineering of this yeast has become far more accessible.

Factors predictive of severe thrombocytopenia and its impact on poor outcomes in Latin American patients with systemic lupus erythematosus: Data from a multiethnic Latin American cohort.

Objective: To examine the predictors of the occurrence of severe thrombocytopenia and its impact on damage accrual and mortality in SLE patients.

Methods: Factors associated with time to severe thrombocytopenia (platelet count ≤20,000/mm) occurring from the onset of SLE symptoms were assessed by Cox proportional hazards regressions. The association of severe thrombocytopenia with mortality was evaluated by logistic regression analyses while its impact on damage was by negative binomial regression.

Analysis of oral and inhalation toxicity of per- and polyfluoroalkylated organic compounds in rats and mice using multivariate QSAR.

Per- and polyfluoroalkylated organic compounds (PFAs) are versatile compounds extensively used in global industries. However, they are also persistent organic pollutants (POPs). This study aimed to develop new models for assessing oral and inhalation toxicity in rat and mice models.

Copper biomarkers and their relationship with dyslipidemia in women with obesity.

Adipose tissue dysfunction influences the development of dyslipidemias associated with obesity, however, the mechanisms are still unclear. In this sense, the literature highlights the role of copper in cholesterol synthesis, contributing to the increase in concentrations of this lipid fraction and consequently to the manifestation of dyslipidemia. The objective of the study was to investigate the relationship between copper parameters and lipid profile markers in women with obesity.

Preserved working memory performance along with subcortical modulation during peri-ictal phases in spontaneous migraine attacks.

Objective: To analyze cognitive performance and brain activation during a working memory task in patients with migraine during various phases of the migraine cycle and compare to healthy participants.

Background: Cognitive difficulties reported during migraine attacks remain poorly understood, despite evidence that the lateral frontoparietal network undergoes reversible disturbances and decreased activation during attacks. Recent findings in resting state functional magnetic resonance imaging suggest that brain areas involved in this network interact with subcortical regions during spontaneous migraine attacks.

Copper biomarkers and their relationship with parameters of insulin resistance in obese women.

Some studies have demonstrated the involvement of high concentrations of copper in the manifestation of insulin resistance in individuals with obesity. The objective of this study was to evaluate the copper nutritional status and its relationship with parameters of glycemic control in women with obesity. An observational case-control clinical study involving 203 women aged between 20 and 50 years, divided into two groups: obese (n = 84) and eutrophic (n = 119).

Unlocking delays: revealing barriers to early diagnosis of childhood central nervous system tumors in an upper-middle-income country.

Purpose: Childhood central nervous system (CNS) tumors tend to have a longer time interval until diagnosis than other pediatric malignancies. The aim is to describe the time to diagnosis among Brazilian pediatric patients treated at a tertiary center and explore associated factors.

Methods: Cross-sectional study; application of questionnaires to parents of children with CNS tumors during outpatient visit or inpatient care.

Isolation and Characterization of Plant-Growth-Promoting Bacteria Associated with Aublet.

Aublet is a floating aquatic plant, capable of absorbing the excess of nutrients and water contaminants and can be used in effluent treatment plants. The ability to survive in degraded areas may be related to the association with beneficial bacteria capable of promoting plant growth. However, little is known about the microbiota associated with this aquatic plant and its potential application to the aquatic environment.