Investigation of Antibacterial Coatings Based on Chitosan/Polyacrylic Acid/Chlorhexidine for Orthopedic Implants.

Antibacterial coatings on model silicon wafers and implants, based on chitosan (CHI), poly(acrylic acid) (PAA), and the antibacterial agent chlorhexidine digluconate (CHX), were obtained using a layer-by-layer assembly method. The surface roughness and 2D and 3D images of the surfaces of CHI/PAA/CHX coatings obtained from different pH assemblies were investigated by atomic force microscopy, revealing that pH 6 enabled optimal inclusion of CHX in the multilayer film. The structure and elemental composition before and after implementation of CHX into the coating were investigated via scanning electron microscopy and energy-dispersive X-ray spectroscopy.

The Effect of Three Complexes of Iodine with Amino Acids on Gene Expression of Model Antibiotic Resistant Microorganisms ATCC BAA-196 and ATCC BAA-39.

Unlabelled: 1.

Background: Iodine is a broad-spectrum antimicrobial disinfectant for topical application. Recent studies have shown promising results on the applicability of an iodine-containing complex, FS-1, against antibiotic-resistant pathogens.

Development and characterization of antibacterial coatings on surgical sutures based on sodium carboxymethyl cellulose/chitosan/chlorhexidine.

The layer-by-layer assembly (LBL) method was used in this work to apply antibacterial coatings to the surface of sutures. The nanofilm was created using sodium carboxymethyl cellulose, chitosan, and chlorhexidine digluconate. Polyethylene terephthalate and polyamide surgical sutures were used as the substrate.

Whole genome sequence data of SCAID WND1-2022 (370).

The whole genome sequence of a hospital infection agent, SCAID WND1-2022 (370), is reported. Raw PacBio generated reads and the genome sequence were deposited at NCBI under BioProject PRJNA754843. The genome comprises two replicons: 4,880,425 bp long chromosome comprising 4524 proteins and functional RNA coding genes and 38,606 bp long plasmid containing 40 CDS.

Component Composition and Biological Activity of Various Extracts of (Boraginaceae).

roots are widely used in traditional medicine to treat various diseases throughout the world. In this study, for the first time, we investigated the component composition and biological activity of various extracts from the roots of collected in the highlands of the Kakpakty Mountains of the Almaty region (Republic of Kazakhstan). Extracts were obtained by three different methods: percolation extraction, ultrasound-assisted extraction, and supercritical carbon dioxide extraction.

Study of Component Composition and Antimicrobial Activity of the Ophthalmic Emulsion Based on the Safflower Flowers ().

The use of medicinal plants has increased significantly in recent years. More than 80% of the world's population uses medicinal plants to treat themselves. Many antibacterial and anti-inflammatory synthetic drugs are available in medical practice.

Complete Genome Sequences of Gram-Positive Opportunistic Pathogens Isolated from Hospitals in Almaty, Kazakhstan.

The appearance of drug-resistant pathogens reduces the therapeutic applicability of antibiotics and increases the rate of hospital infections among patients. Complete genome sequences of four Gram-positive clinical isolates of Streptococcus and Staphylococcus were obtained and analyzed to serve as model microorganisms for further studies on drug-induced antibiotic resistance reversion.

Complete Genome Sequences of Gram-Negative Opportunistic Pathogens Isolated in Hospitals in Almaty, Kazakhstan.

The problem of nosocomial infections is growing due to the introduction of new treatment regimens involving immunosuppressive drugs. The genomes of seven Gram-negative clinical isolates of Escherichia, Klebsiella, and Pseudomonas were sequenced and analyzed in this study to serve as model microorganisms to study drug-induced antibiotic resistance reversion.

Transcriptomics and methylomics study on the effect of iodine-containing drug FS-1 on ATCC BAA-196.

Promising results on application of iodine-containing nano-micelles, FS-1, against antibiotic-resistant was demonstrated. RNA sequencing for transcriptomics and the complete genome sequencing by SMRT PacBio were followed by genome assembly and methylomics. FS-1-treated showed an increased susceptibility to antibiotics ampicillin and gentamicin.

Chemical Composition and Antimicrobial Activity of Subcritical CO Extract of L. (Brassicaceae).

The genus L. from Brassicaceae Burnett. family covers over 150 species with an almost cosmopolitan spread.

The Effect of Iodine-Containing Nano-Micelles, FS-1, on Antibiotic Resistance, Gene Expression and Epigenetic Modifications in the Genome of Multidrug Resistant MRSA Strain ATCC BAA-39.

Application of supplementary drugs which increase susceptibility of pathogenic bacteria to antibiotics is a promising yet unexplored approach to overcome the global problem of multidrug-resistant infections. The discovery of a new drug, an iodine-containing nano-molecular complex FS-1, which has proven to improve susceptibility to antibiotics in various pathogens, including MRSA strain ATCC BAA-39, allowed studying this phenomenon. Chromosomal DNA and total RNA samples extracted from the FS-1 treated strain (FS) and from the negative control (NC) cultures were sequenced by PacBio SMRT and Ion Torrent technologies, respectively.

Complete Genome Sequence of Collection Strain Acinetobacter baumannii ATCC BAA-1790, Used as a Model To Study the Antibiotic Resistance Reversion Induced by Iodine-Containing Complexes.

The strain ATCC BAA-1790 was sequenced as a model for nosocomial multidrug-resistant infections. Long-read PacBio sequencing revealed a circular chromosome of 3,963,235 bp with two horizontally transferred genomic islands and a 67,023-bp plasmid. Multiple antibiotic resistance genes and genome methylation patterns were identified.

Complete Genome Sequence of a Multidrug-Resistant Strain, Escherichia coli ATCC BAA-196, as a Model for Studying Induced Antibiotic Resistance Reversion.

Here, we report the complete genome sequence of the multidrug-resistant strain ATCC BAA-196, a model organism used for studying possible antibiotic resistance reversion induced by FS-1, an iodine-containing complex. Two genomes, representing FS-1-treated and negative-control variants and composed of a chromosome and several plasmids, were assembled.

Assembly of Complete Genome Sequences of Negative-Control and Experimental Strain Variants of Staphylococcus aureus ATCC BAA-39 Selected under the Effect of the Drug FS-1, Which Induces Antibiotic Resistance Reversion.

ATCC BAA-39 is the reference organism for a multidrug-resistant (MRSA) strain that was used to study drug-induced resistance reversion by an iodine-containing nanomolecular complex, FS-1. PacBio sequencing was performed on both the experimental and control strains, followed by genome assembly, variant calling, and DNA modification profiling.