Grapevine Shoot Tip Cryopreservation and Cryotherapy: Secure Storage of Disease-Free Plants.

Grapevine ( spp.) is one of the most economically important temperate fruit crops. Grapevine breeding programs require access to high-quality cultivars and wild species, which may be maintained within genebanks.

Screening of Croatian Native Grapevine Varieties for Susceptibility to Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging.

In the era of sustainable grapevine production, there is a growing demand to define differences between varieties in susceptibility to downy mildew. Croatia, as a country with a long tradition of grapevine cultivation, preserves a large number of native grapevine varieties. A leaf disc bioassay has been conducted on 25 of them to define their response to downy mildew, according to the International Organisation of Vine and Wine (OIV) descriptor 452-1, together with the stress response of the leaf discs using chlorophyll fluorescence and multispectral imaging with 11 parameters included.

Grapevine as a Rich Source of Polyphenolic Compounds.

Grapes are rich in primary and secondary metabolites. Among the secondary metabolites, polyphenolic compounds are the most abundant in grape berries. Besides their important impacts on grape and wine quality, this class of compounds has beneficial effects on human health.

Effect of shooting medium and source of material on grapevine (Vitis vinifera L.) shoot tip recovery after cryopreservation.

Background: Selecting experimental material at the optimal physiological stage is of paramount importance for successful cryopreservation.

Objective: The study was to investigate the effect of the physiological state of grapevine buds on their regrowth after liquid nitrogen exposure.

Methods: In a first set of experiments, we tested the regrowth of cryopreserved buds sampled from microcuttings cultured on shooting medium containing benzylaminopurine or zeatin riboside for various durations.