Protein ubiquitin in its +7 charge state microhydrated by 5 and 10 water molecules has been interrogated in the gas phase by cold ion UV/IR spectroscopy. The complexes were formed either by condensing water onto the unfolded bare proteins in a temperature-controlled ion trap or by incomplete dehydration of the folded proteins. In the case of cryogenic condensation, the UV spectra of the complexes exhibit a resolved vibrational structure, which looks similar to the spectrum of bare unfolded ubiquitin.
View Article and Find Full Text PDFThe geometry of biomolecules isolated in the gas phase usually differs substantially from their native structures in aqueous solution, which are the only ones truly relevant to life science. To connect the high resolution of cold ion spectroscopy that can be achieved in the gas phase and the key role of intermolecular hydrogen bonds that shape biomolecules in water, we study protonated tryptophan microhydrated by 1-6 water molecules. IR/UV spectra measured with the same instrument under similar conditions appear to be identical for the complexes of the same size produced by soft dehydration and cryogenic condensation methods.
View Article and Find Full Text PDFAmino acids and peptides generally exhibit zwitterionic forms with salt bridge (SB) structures in solution but charge-solvated (CS) motifs in the gas phase. Here, we report a study of non-covalent complexes of the protonated amino acid arginine, ArgH(HO) ( = 1-5), produced in the gas phase from an aqueous solution with a controlled number of retained water molecules. These complexes were probed by cold ion spectroscopy and treated by quantum chemistry.
View Article and Find Full Text PDFWe present herein the design of a nano-electrospray ion source capable of reliable generation of large quantities of microsolvated ions. The source is based on a triple molecular skimmer scheme and can be quickly tuned to generate bare ions or their ionic complexes with up to more than 100 solvent molecules retained from solution. The performance of this source is illustrated by recording the mass spectra of distributions of ionic complexes of protonated water, amino acids, and a small protein ubiquitin.
View Article and Find Full Text PDFThe difference in functionality of many isomeric biomolecules requires their analytical identification for life science studies. We present a universal approach for quantitative identification of different small- to medium-sized isomeric biomolecules that can be brought to the gas phase from solution by electrospray ionization (ESI). The method involves infrared (IR) fragment cold ion spectroscopy of analyte molecules that are incompletely desolvated by soft ESI.
View Article and Find Full Text PDFCharacterization of native structures of proteins in the gas phase remains challenging due to the unpredictable conformational changes the molecules undergo during desolvation and ionization. We spectroscopically studied cryogenically cooled protonated protein ubiquitin and its microhydrated complexes prepared in the gas phase in a range of charge states under different ionization conditions. The UV spectra appear vibrationally resolved for the unfolded protein, but become redshifted and smooth for the native-like structures of ubiquitin.
View Article and Find Full Text PDFThe native-like structures of protonated glycine and peptide GlyH were elucidated using cold ion IR spectroscopy of these biomolecules hydrated by a controlled number of water molecules. The complexes were generated directly from an aqueous solution using gentle electrospray ionization. Already with a single retained water molecule, GlyH exhibits the native-like structure characterized by a lack of intramolecular hydrogen bonds.
View Article and Find Full Text PDFAmaranth flour--a product of amaranth seeds processing--is a valuable industrial raw material that has an unique chemical composition and may be used for nutrition of people suffering from intolerance to traditional cereals protein, including celiac disease patients. The research aim was to study the composition of amaranth flour of two types compared with semolina which is traditionally used for nutrition by Russian population, as well as to compare the composition of milk amaranth flour porridge with milk semolina porridge. The composition of amaranth whole-ground flour and amaranth flour of premium grade processed from amaranth seeds grown in Voronezh region has been researched.
View Article and Find Full Text PDFTreatment results of 5 patients with unformed intestinal fistulae are represented. High unformed intestinal fistulae are acknowledged to be completely unsuitable for conservative treatment and should be operated on. Complex treatment should include complete parenteral feeding, adequate fecal diversion with the use of aspirational drainage.
View Article and Find Full Text PDFWe have discussed the individual anthyhelicobacter treatment duration from 7 to 14 days for functional dyspeptic syndrome in children and adolescent. We have performed 3-year follow-up results in different patients group: H. pylori reinfection, H.
View Article and Find Full Text PDFTreatment results of 336 patients in critical state who had required prolonged (3-115 days) artificial pulmonary ventilation were analyzed. 3 groups were defined: 98 patients with complications after operations on thoracic and abdominal organs and organ failure development; 55 patients with surgical infection and sepsis; 183 patients with extensive burning injury III AB-IV degrees. Particular features of prolonged artificial pulmonary ventilation were studied.
View Article and Find Full Text PDFVopr Kurortol Fizioter Lech Fiz Kult
February 2009
The efficiency of transcutaneous magneto-laser treatment as a component of combined therapy of chronic gastritis in children and adolescents (aged 5-17 years) was compared with that of pharmacotherapy and low-intensity laser therapy. The patients were allocated to three groups of 25 persons each. Patients of group 1 were given only drug therapy, those in group 2 were treated with pharmaceuticals and low-intensity laser therapy.
View Article and Find Full Text PDFNew medical human lactoferrin product called Laprot possessing antioxidant, detoxicant, anti-inflammatory immunomodulating properties was developed and registered (serial number LS-002374) in the P.A. Hertsen Institute.
View Article and Find Full Text PDFThe study was undertaken to define the main causes of nosocomial infection and the possible ways of its prevention in a resuscitation and intensive care unit (RICU). In 1999 to 2004, intensive therapy was performed in 408 patients (244 males and 163 females) aged 18 to 60 years (54%) and above 60 years (45%) who had severe surgical infection (severe sepsis). Most patients aged over 60 years had one concomitant disease or more.
View Article and Find Full Text PDFConduction anesthesia-induced central hemodynamic changes were studied in 20 patients with pyonecrotic forms of the diabetic foot and coronary heart disease (CHD). There was a 19% reduction in cardiac output and a 25% increase in total peripheral vascular resistance, as compared with the baseline values, the level of blood pressure being unchanged. The authors proposed to include droperidole into premedication and intraoperative perlinganite infusion for postload reduction, which stabilized central hemodynamics during conduction anesthesia in patients with diabetes mellitus and CHD.
View Article and Find Full Text PDFRegional anesthesia was studied for its impact on the functional status of a peripheral nerve in 20 patients with purulent diseases of the lower extremities in the presence of diabetes mellitus. The impact of conservative therapy on the peripheral nerve was also studied in these patients. There was no negative impact of regional methods of anesthesia on the function of the nerve, its improvement due to treatment with alpha-lipoic acid and serotonin adipinate.
View Article and Find Full Text PDFKhirurgiia (Mosk)
December 2002
Anesteziol Reanimatol
December 2002
The possibility of objectively evaluating the severity of the clinical status of patients with surgical infection is discussed. A retrospective and prospective analysis of clinical laboratory values is carried out in 196 patients with surgical sepsis. SAPS score indicated a statistically significant difference in the state of two groups of patients (survivors and dead).
View Article and Find Full Text PDFAnesteziol Reanimatol
December 2002
Central hemodynamics and physiological profiles were followed up during epidural anesthesia for surgical treatment of pyonecrotic forms of diabetic foot. Modification of epidural anesthesia is described. Hemodynamic stability and adequacy of anesthesia in patients with severe concomitant diseases and chronic specific complications of diabetes mellitus are demonstrated.
View Article and Find Full Text PDFDuring the period of 1987-1998 operations for obliterating diseases of the peripheral arteries were performed on 104 patients having critical ischemia of the diseased extremity. The microsurgical technique was used. Indirect revascularization of the ischemic extremity with the soft tissue flap was fulfilled in 56 patients, femoro-distal-crural bypass was used in 38 patients, in 11 of them the bypass was "unloaded" into the flap of the broadest dorsal muscle, in 10 patients arterialization of the origins of the major subcutaneous veins was made.
View Article and Find Full Text PDF500 anesthesias were performed in patients with surgical diseases in the presence of diabetes millitus (90% of patients with II type diabetes). The methods of intravenous anesthesia are proposed for short-term procedures with preservation of spontaneous ventilation and stable hemodynamics. The method of epidural anesthesia with introduction of major dose of anesthetic in two stages was developed for the operation on the lower extermities, it provides for stable hemodynamics and adequate anesthesia.
View Article and Find Full Text PDFThe analysis of the treatment of 900 patients with large festered wounds of various genesis and location for the period from 1973 to 1998 years in the intensive care department has shown, that infection of respiratory ways is encountered in 30% of cases (in patients with nonsporeforming anaerobic bacteria--in 11-12%), bacteriuria--in 70-80%, bacteriamia--in 75% of patients with sepsis. In acute pyogenous diseases of soft tissues microbes from the wounds in monoculture were isolated out in 83.3% of cases, associations of gram-positive and gram-negative bacteria--in 16.
View Article and Find Full Text PDFThe experience in treatment of 740 patients with surgical sepsis is presented. The problems of the diagnosis, pathogenesis of sepsis, clinical and laboratory features of its course, and disturbances of homeostasis are discussed. Score systems for evaluation of the severity of patients' conditions are analysed.
View Article and Find Full Text PDFKhirurgiia (Mosk)
February 1999
In 26 patients (female 11, males--15) with diabetic angiopathy (necrosis of the foot or toes) local measurement of pO2 by transcutaneous mode was carried out before and during introduction of serotonine adipinate. Simultaneously with evaluation of pO2 in 14 patients electromyography (EMG) was made. Serotonine adipinate was introduced intravenously with a speed of 5.
View Article and Find Full Text PDF