Publications by authors named "Zuzana Varchulova Novakova"
Article Synopsis
- - The study compares two decellularization methods (enzyme-detergent and detergent-detergent) to see how they affect the structure and mechanics of human urethral tissue, using samples from 18 individuals.
- - Both methods successfully removed cellular content while maintaining essential extracellular matrix components like collagen and elastic fibers, indicating they preserved the tissue's structural integrity.
- - Biomechanical tests showed that the detergent-detergent method resulted in lower stress levels compared to native tissues, and both methods had reduced elasticity, but none were toxic to stem cells, suggesting they are viable for tissue engineering and biobanking.
Article Synopsis
- * They analyzed 79 patient samples using real-time PCR, finding that FOXA1, GATA3, and TP53 were more expressed in cancerous tissues compared to non-tumor tissues, with variations noted between high-grade and low-grade tumors.
- * The findings suggest that higher expression levels of TP53, GATA3, and lower levels of FOXA1 in patients correlate with recurrence of bladder cancer, indicating these markers could help assess cancer risk over time.
Article Synopsis
- - Urine-derived stem cells (UdSCs) show significant anti-inflammatory and immune-modulating properties, but their effectiveness in treating autoimmune diseases like rheumatoid arthritis (RA) is still under investigation.
- - In a study, UdSCs were tested in an RA-like environment created by exposing them to rheumatoid synovial fluids, serum deprivation, and acidic conditions, revealing that while UdSCs proliferated normally, acidic conditions harmed other stem cells.
- - Exposure to RA-related elements increased UdSCs' secretion of various immunomodulatory factors and enhanced their ability to suppress the proliferation of activated immune cells, suggesting that UdSCs could be a promising therapeutic option for RA when delivered directly into the affected joints.
Urothelial bladder carcinoma (UC) ranks among the top ten most commonly diagnosed cancers worldwide on an annual basis. The standardized classification system for urothelial bladder tumors is the Tumor, Node, Metastasis classification, which reflects differences between non-muscle-invasive bladder carcinoma (NMIBC) and muscle-invasive bladder carcinoma (MIBC) and it depends on the extent to which tumor has infiltrated the bladder wall and other tissues and organs. NMIBC and MIBC exhibit great intrinsic heterogeneity regarding different prognoses, survival, progression, and treatment outcomes.
View Article and Find Full Text PDFBladder cancer (BC) is the 10th most frequent cancer in the world. The initial diagnosis and surveillance of BC require a combination of invasive and non-invasive methods, which are costly and suffer from several limitations. Cystoscopy with urine cytology and histological examination presents the standard diagnostic approach.
View Article and Find Full Text PDFAlthough the incidence varies with age and gender, urothelial bladder cancer is a relatively frequently occurring malignancy with variable clinical behavior that often has high recurrence rates. In this study, we analyzed the tumor tissues of 224 patients with pTa, pT1, and pT2 urinary bladder cancer. We performed a histomorphologic analysis and immunohistochemistry for p53, Ki-67, and E-cadherin, which were selected as markers of the malignant process.
View Article and Find Full Text PDFObjectives: Recently, it was demonstrated that human periodontal ligament stem cells have great potential for tissue engineering and regenerative medicine not limited to oro-maxillofacial region. They are easily accessible and they may be expanded under in vitro conditions. In this study we assessed the effect of long-term cultivation on the selected biological and morphological properties of human periodontal ligament stem cells.
View Article and Find Full Text PDFSomatic stem cells possess unique properties of self-renewal and plasticity which make them promising candidates for use in tissue engineering and regenerative medicine, in addition to serving as efficient delivery vehicles in site-specific therapy. In the case of therapeutic application, it is essential to isolate and culture stem cells in vitro, to obtain them in sufficient quantities. Although long-term cultivation provides an adequate number of cells, it has been shown that this approach is associated with increased risk of transformation of cultured cells, which presents a significant biological hazard.
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