We describe the course of infection of Cryptosporidium andersoni LI03, originally isolated from cattle, in outbred Gerbillus gerbillus (Lesser Egyptian Gerbil), Meriones unguiculatus (Mongolian gerbil), and Meriones tristrami (Tristram's jird). While both Meriones spp. partially cleared the infection and shed a low number of oocysts (less than 15,000 oocysts per gram (OPG)), chronic infection with a mean infection intensity reaching 200,000 OPG was observed in G.
View Article and Find Full Text PDFHeat shock proteins (Hsp) are important for the stability and function of cell proteins and thus for cell survival under physiological as well as stress conditions. Hsps were also reported to play an important role in tumorogenesis including leukemias. In this study we followed up Hsp70 and 90 protein levels in samples from patients with chronic myeloid leukemia (CML) to evaluate these Hsps with regard to their ability to characterize the disease status and disease prognosis.
View Article and Find Full Text PDFA total of 995 samples from slaughtered dairy cattle (6 months and older) were collected during two consecutive years (from 2007 to 2008), stained by aniline-carbol-methyl violet and examined microscopically. DNA was extracted from Cryptosporidium positive samples and from 200 randomly selected microscopically negative samples. Nested PCR was performed to amplify the partial SSU rRNA and GP60 genes of Cryptosporidium that were subsequently digested by SspI, VspI and MboII restriction enzymes to determine the Cryptosporidium species and genotype present.
View Article and Find Full Text PDFThe infectivity and pathogenicity of Cryptosporidium andersoni (bovine isolate) for neonatal and adult southern multimammate mice (Mastomys coucha) was studied using transmission experiments. C. andersoni isolate used in this study was not infective for BALB/c mice, but experimental infection proved susceptibility of neonatal and adult M.
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