To investigate the proteomic differences between the capacitated and non-capacitated sperm of the Yanbian yellow cattle, semen was collected from three Yanbian yellow cattle, aged 3-5 years, pooled, and divided into capacitated and non-capacitated groups (n = 9) to be analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and liquid chromatography-mass spectrometry. Gene ontology enrichment, Kyoto Encyclopedia of Genes and Genomes pathway enrichment, and protein-protein interactions were used for bioinformatics analysis. The results showed that 598 were described as differentially expressed proteins during sperm capacitation.
View Article and Find Full Text PDFThis study was undertaken to investigate the dynamics of protein ubiquitination in pig gametes and their micro-environments, as well as to explore the action of deubiquitinases (DUBs) in sperm-oocyte binding. Protein ubiquitination states were evaluated by in the ejaculated sperm, seminal plasma, epididymal sperm, oocytes, zona pellucida (ZP) and follicular fluid (FF) by western blotting. Different concentrations of PR-619, a non-selective inhibitor of DUBs, were used to treat oocytes during in vitro maturation (IVM), the maturation rate, amount of ubiquitinated ZP proteins, and ZP solubility were assessed.
View Article and Find Full Text PDFHua Xi Kou Qiang Yi Xue Za Zhi
December 2004
Objective: To investigate the influence of mold temperature on the mechanical properties of Ti-Zr alloy castings.
Methods: Ti-Zr alloy was cast into a mold which was made of a zircon (ZrO2.SiO2) investment with a casting machine (China) designed as vacuum, pressure and centrifuge.