Publications by authors named "Zuo-Ying Xiahou"
Multiplex polymerase chain reaction (PCR) of microsatellite loci allows for simultaneous amplification of two or more pairs of primers in a single PCR reaction; hence, it is cost and time effective. However, very few attempts have been reported in non-model species. In this study, by combining a genome-based de novo development and cross-species application approach, a multiplex PCR system comprising 5 PCR reactions of 33 microsatellites consisting of 26 novel genomic and 7 literature-sourced loci was tested for polymorphisms, cross-species transferability, and the ability to assess genetic diversity and population structure of three walnut species ( spp.
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- Walnuts are in high demand for their nutritional benefits and medicinal uses, prompting intensified breeding efforts in areas like southwest China to create better cultivars.
- Researchers genotyped 362 walnut tree samples (including both cultivated and wild) using microsatellite markers, revealing low genetic diversity and high clonality among cultivars, suggesting a narrow genetic base for breeding.
- The study identified a key genetic group linked to the 'Yangbidapao' landrace and emphasized the need to incorporate more diverse genetic sources, including wild walnuts, to enhance breeding programs and conservation efforts in the region.
Premise: A novel set of microsatellite markers was developed for (Juglandaceae), an endemic walnut species in southwestern China, to facilitate cultivar identification and future investigations into the genetic structure and domestication history of this species and its close relatives.
Methods And Results: We developed 32 microsatellite loci for using genomic data and used them to examine 60 individuals from three natural populations. A high level of polymorphism was detected by these primers, with up to eight alleles observed per locus, and an average of four alleles across populations.