Diversity in the section Compressa of the genus Chaetoceros (Bacillariophyceae), with description of two new species from Chinese warm waters.

Monoclonal strains of Chaetoceros species were collected from Chinese warm waters. Vegetative cells and resting spores were examined using light microscopy as well as scanning and transmission electron microscopy. Combined morphological and DNA sequence data from the hypervariable D1-D3 region of the nuclear ribosomal large subunit showed the presence of two new species within the section Compressa, herein described as Chaetoceros bifurcatus sp.

Chaetoceros pauciramosus sp. nov. (Bacillariophyceae), a Widely Distributed Brackish Water Species in the C. lorenzianus Complex.

To understand the diversity of the diatom genus Chaetoceros Ehrenberg, the C. lorenzianus complex was previously chosen as a model and three new species were described. In the present study, yet another new species, Chaetoceros pauciramosus sp.

The Expression of HCG Epitope Fused to Hepatits B Virus Core Antigen.

The DNA fragments encoding HGC-beta-37-CTP was amplified by PCR and fused to the core gene of HBV at the position of amino Acid 1(N-terminal fusion, pCn-HCG), 154(C-terminal fusion, pCc-HCG), 75-83(internal fusion,pCm-HCG), and both 75-83 and 154 (pC-HCG2) respectively. The fused genes were expressed in E. coli, and the antigenicity of both HBcAg and HCG as well as the expression level were analyzed.

The Fusion Expression of HBV preS Epitopes and the Core Antigen.

The DNA fragments encoding the preS epitopes of HBV surface antigen were fused to the HBc gene and expressed in E. coli under the control of the tac promoter. The products were analyzed by ELISA and Western blotting, which confirmed that the hybrid proteins were expressed as expected.