Teratoma in an adolescent with malignant transformation into embryonal rhabdomyosarcoma: case report.

Background: The somatic type tumors are occasionally found in nonseminomatous germ cell tumors in men. These malignancies are presumed to arise from malignant transformation (MT) of teratoma or by differentiation of totipotential germ cell.

Observation: A case of MT of germ cell tumor in 17-year-old male into embryonal rhabdomyosarcoma is described.

Radioguided occult lesion localisation in combination with detection of the sentinel lymph node in non-palpable breast cancer tumours.

Purpose Of The Investigation: The aim of study was to determine the efficacy of radioguided occult lesion localisation (ROLL) for non-palpable invasive breast cancer combined with sentinel lymph node biopsy (SLNB) and to compare the amount of tissue excised by radioguided navigation versus the hook-wire technique.

Methods: We injected 45 MBq of radiolabelled technetium intratumourally and 15 MBq subdermally 18 hours before surgery in 21 women with bioptically verified non-palpable breast cancer. We identified by gamma probe non-palpable tumours, which were excised, followed by identification and excision of the sentinel lymph node.

[New method for detection of nonpalpable breast cancer].

Objective: The objective of the study was to evaluate the feasibility of radio guided occult lesion localization (ROLL) in the cases of nonpalpable breast cancers with sentinel lymph node biopsy.

Design: Prospective clinical study

Setting: Department of Gynecology and Obstetrics, 2nd Medical Faculty and Teaching Hospital, Praha

Methods: Thirty-eight patients with nonpalpable breast cancer diagnosed by core cut biopsy underwent an injection of the radiopharmaceutical 18-20 hours before surgery. The dose of the radiopharmaceutical was 45 MBg into tumor and 15 MBg subdermaly.

Mixed germ cell sex cord-stromal tumors of the testis and ovary. Morphological, immunohistochemical, and molecular genetic study of seven cases.

We present the morphological, immunohistochemical, and molecular genetic features of three cases of testicular and four cases of ovarian mixed germ cell sex cord-stromal tumors (MGSCT). The germ cells in the testicular MGSCTs morphologically differed from those in classical seminomas by lacking the typical "square off" quality of the nuclei. In contrast to the nuclei in classical seminomas, their size in testicular MGSCTs was smaller and nucleoli were inconspicuous and the cytoplasm was Periodic Acid-Schiff (PAS) negative.

Prenatal diagnosis of occipital dermal sinus associated with hemangioma using ultrasound and MRI. Case report.

Objective: We report a case of prenatal diagnosis and postpartum management of a subcutaneous tumor without intracranial communication.

Methods: An occipital tumor without intracranial communication was found on ultrasound scan in the 21st week of pregnancy. Using MRI, the diagnosis was confirmed.

[Sentinel lymphatic node biopsy for breast cancer in practice].

Objective: This paper reviewed the feasibility and accuracy of sentinel lymph node status in women with breast cancer.

Design: Clinical retrospective study.

Setting: Dept.

[Mixed germ cell tumours of the ovary in childhood and adolescence].

Mixed germ cell tumours of the ovary are rare malignant neoplasms containing combinations of two or more types of germ cell elements. The aim of the study was to review biopsy examinations, medical records, treatment strategy, follow-up and outcome of all girls treated for mixed germ cell tumour of the ovary at the Department of Pediatric Oncology, University Hospital Motol during the period 1979-2002. Archival slides of all tumours were reviewed and tumours were classified according to the WHO system.

[Ovarian dysgerminoma in children and adolescents. Retrospective clinico-pathologic study].

Background: To review the treatment strategy, follow up and outcome for all patients with pure ovarian dysgerminoma treated in childhood and adolescence.

Methods And Results: Twenty-one patients younger than 18 years were treated between 1979-2002 in Faculty Hospital Motol for newly diagnosed pure ovarian dysgerminoma. Patients were included into the cohort on the basis of revision of archival biopsy specimens deposited in Institute of Pathology and in Molecular Medicine tumor registry.

Detection of the sentinel node in breast carcinoma using method of a single subcutaneous injection of radiopharmaceutical.

The objective of this work is retrospective evaluation of results of the intraoperative detection of sentinel node in breast carcinoma after a single subcutaneous injection of radiopharmaceutical (RF) within a two-day protocol. From May/2001 to June/2002, lymphoscintigraphy of the sentinel node (SN) and its subsequent radioguided intraoperative detection (RGS) was performed in 43 women having stage T1-T2, N0 breast carcinoma. The static scans in the anterior and relevant lateral projections were performed using a gamma camera at approximately 30-minute intervals after the subcutaneous administration of 15 MBq 99mTc Senti-Scint, until the SN was displayed.

[Hormone replacement therapy, proliferation in the breast and risk of carcinoma].

Objective: The objective of the study was to investigate the changes of histopathological and immunohistochemical parameters of breast tissue among the HRT users.

Design: Prospective, pilot study.

Setting: Departments of Gynecology and Obstetrics, Pathology and Radiology of the 2nd Medical School, Charles University and the Teaching Hospital Motol, Praha 5, Czech Republic.

[Superficial epithelial-stromal ovarian tumors in girls].

Superficial epithelial-stromal ovarian tumours are unusual in adolescent girls (when compared with adult women) and extremely rare before menarche. A group of 40 girls with such tumours was chosen among 180 cases (22%) of ovarian tumours in childhood and adolescent age. All of them were between 9 and 18 years with one exception of a 5-year old girl.

[Parovarian cysts and tumors in adolescent girls].

Biopsy in 30 girls suffering from parovarial lesions showed 27 parovarial cysts and 3 parovarial tumours. Girls' age varied between 12 and 17--except an 1-year-old infant. 13 girls had to be operated on paramesonephric unilocular usually solitary cysts with a tubal sort of lining.

[Histological structure of the peniform clitoris in congenital adrenogenital syndrome].

Peniform clitoris in 50 girls suffering from congenital adrenogenital syndrome were subtotally removed at the age of 4-6 years and studied by biopsy. A corpus spongiosum more or less developed was found in all cases beneath the corpora cavernosa in the bottom part of corpus clitoridis, epithelial urethral rudiments represented by single or multiple short tubules with transitional epithelium and mucinous glands were found in 46 cases; they were localized in corpus spongiosum or close to it in loose connective tissue. Other urethral rudiments occurring in glans clitoridis were represented by long slight pegs of squamous epithelium extending into corpus spongiosum glandis.

Fatal infantile hypertrophic cardiomyopathy secondary to deficiency of heart specific phosphorylase b kinase.

We describe here a male infant with a rare form of glycogenosis caused by deficiency of heart specific phosphorylase b kinase. The disease phenotype was characterized by severe glycogenosis restricted to the heart muscle with secondary rapidly progressive hypertrophic cardiomyopathy causing death at the age of 47 days.

[Juvenile granulosa cell tumor with subsequent occurrence of gastrointestinal polyposis, subcutaneous lipomatosis and nodular goiter].

A juvenile tumour from granulosa cells (M-8622/1), 13 x 8 x 6 cm, in the right ovary in a three-month-old girl produced some symptoms of pseudopubertas praecox isosexualis which disappeared after operation. Microscopic examination of the tumour revealed in addition to typical structures a less common differentiation to Sertoli cells. Despite actinotherapy and chemotherapy one and a half years after the onset of the disease X-ray examination revealed metastases in the lungs which were successfully cured by further doses of the above two types of treatment.

[An ovarian tumor with structural gonadoblastoma, dysgerminoma and choriocarcinoma].

A 14 1/2-year old girl with menometrorrhagia followed by amenorrhea and abdominal pain had a pelvic resistance with limited mobility. Histology of a left ovarian tumour showed gonadoblastoma turning to dysgerminoma and associated with choriocarcinoma (M-9073/1, M-9060/3, M-90101/3). Genuine ovarian tissue was hypoplastic on both sides.

Mixed germ cell-sex cord stromal tumor with heterologous structures.

A mixed germ cell-sex cord stromal tumor developed in the right ovary of a 4-year-old girl. The patient's cells contained sex chromatin and the karyotype was 46,XX. Clinically, she exhibited mild isosexual pseudopuberty.

[Gonadal dysgenesis].

The authors describe six patients, female phenotype with karyotype 45X/46XY. Two girls suffered from pure gonadal dysgenesis, four from mixed gonadal dysgenesis. The authors emphasize the intact axis hypothalamus-pituitary gonads.

[Juvenile granulosa cell tumor].

Granulosa cell juvenile tumours, defined in 1976 by Scully as a separate unit are found mostly in girls during the first two decades of life. On inspection under an optic microscope they are characterized by the presence of non-differentiated "blastemoid" structures and the formation of solid follicular formations and cysts. In our first case we observed a bilateral juvenile tumour made up of granulosa cells in a 8-month-old girl where the tumour produced symptoms of pseudopubertas praecox isosexualis.

[Ultrastructure of foamy and pigmented cells in the female breast].

Electronmicroscopy was performed on 5 surgical specimens from the mammary gland of 21-, 34-, 49-, 49-, and 51-years-old women. Histological diagnosis of mammary dysplasia was established in all of patients. Ultrastructure of foamy cells showed lipid vacuoles without obvious limiting membrane, lysosomal vacuoles with annulate and vesiculate membranous structures, lamellar bodies and lipofuscin inclusions which prevailed in pigmented cells.