ESBL Producing E. coli in Chickens and Poultry Farms Environment in Selangor, Malaysia: A Cross-Sectional Study on Their Occurrence and Associated Risk Factors With Environment and Public Health Importance.

Introduction: Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) are widespread multidrug-resistant zoonotic bacteria that threaten animal production, food safety and antimicrobial therapy worldwide including Malaysia. Poultry has been reported as one of the pathways for human exposure to ESBL-EC. There has been little research on the occurrence of ESBL-EC within the Malaysian poultry food chain.

Screening of in swine workers of selected states in Peninsular Malaysia.

Background And Aim: is a zoonotic pathogen that is highly associated with contact between live pigs and raw pig material. In view of the recent reports of human infections in Malaysia, epidemiological data on the status of in the human population, especially among people working closely with pigs and/or raw pork, should be provided. The aim of this study was to detect among individuals working in the swine industry in several major pig production areas in Peninsular Malaysia.

Phenotypic and genotypic characterization of colistin-resistant Escherichia Coli with mcr-4, mcr-5, mcr-6, and mcr-9 genes from broiler chicken and farm environment.

Background: Colistin is an antibiotic used as a last-resort to treat multidrug-resistant Gram-negative bacterial infections. Colistin had been used for a long time in veterinary medicine for disease control and as a growth promoter in food-producing animals. This excessive use of colistin in food animals causes an increase in colistin resistance.

Virulence gene profiles and antimicrobial susceptibility of Brancaster from chicken.

Background: The current conventional serotyping based on antigen-antisera agglutination could not provide a better understanding of the potential pathogenicity of subsp. serovar Brancaster. Surveillance data from Malaysian poultry farms indicated an increase in its presence over the years.

The occurrence and molecular detection of and genes in isolated from poultry and poultry meats in Malaysia.

The advent of antimicrobials-resistant (AMR), including colistin-resistant bacteria, poses a significant challenge to animal and human health, food safety, socio-economic growth, and the global environment. This study aimed to ascertain the colistin resistance prevalence and molecular mechanisms of colistin resistance in . The colistin resistance was determined using broth microdilution assay, PCR; and Sanger sequencing of genes responsible for colistin resistance in ( = 627), including (436) spp.

The global profile of antibiotic resistance in bacteria isolated from goats and sheep: A systematic review.

Background And Aim: Antibiotic resistance has become an issue of global importance due to increasing levels of bacterial infections worldwide. Farm management and usage of antibiotics in livestock are known risk factors associated with the increase in global levels of antibiotic resistance. Goats and sheep are examples of livestock with large populations.

Antimicrobial Resistance Profiles and Co-Existence of Multiple Antimicrobial Resistance Genes in -Harbouring Colistin-Resistant Isolates Recovered from Poultry and Poultry Meats in Malaysia.

The co-existence of the colistin resistance () gene with multiple drug-resistance genes has raised concerns about the possibility of the development of pan-drug-resistant bacteria that will complicate treatment. This study aimed to investigate the antibiotic resistance profiles and co-existence of antibiotic resistance genes among the colistin-resistant isolates recovered from poultry and poultry meats. The antibiotic susceptibility to various classes of antibiotics was performed using the Kirby-Bauer disk diffusion method and selected antimicrobial resistance genes were detected using PCR in a total of 54 colistin-resistant isolates including (.

Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia.

Antimicrobial resistance is one of the major public health threats globally. This challenge has been aggravated with the overuse and misuse of antibiotics in food animals and humans. The present study aimed to investigate the prevalence of Extended-Spectrum β-lactamase (ESBL) genes in Escherichia coli (E.

Prevalence and multidrug-resistant profile of methicillin-resistant and methicillin-resistant in dogs, cats, and pet owners in Malaysia.

Background And Aim: and are widespread skin and mucous membrane colonizers and may cause opportunistic infections in humans and animals. This study aimed to identify and characterize methicillin-resistant (MRSA) and methicillin-resistant (MRSP) isolates from domestic and stray dogs and cats and pet owners in Malaysia using molecular epidemiology and antimicrobial profiling.

Materials And Methods: Three hundred and fifty oral and nasal swabs were taken from pet and stray dogs and cats and pet owners; all samples were subjected to culture and biochemical tests and polymerase chain reaction; the selected isolates were put through disk diffusion test and multilocus sequence typing.

Seroprevalence and Factors Associated with , , and Infections in Cattle and Goats in Selangor, Malaysia.

Apicomplexan parasites such as , , and are widely recognized as causes of production diseases in ruminants. This study aimed to investigate the serological occurrence of , , and in cattle and goats from smallholder farms in Selangor, Malaysia. A cross-sectional study was conducted on 19 farms by collecting 404 bovine (n = 225) and caprine (n = 179) serum samples, which were then essayed for , , and antibodies using commercially available ELISA test kits.

Pathogenicity of subspecies phage type 1 in one-day-old specific pathogen-free chicks.

Background: The studies about infection in newly hatched chicks were not extensive.

Aim: The objective of this study was to determine the pathogenicity of subspecies serovar (SE) phage type (PT) 1 in one-day-old specific pathogen-free (SPF) chicks.

Methods: Seventy, one-day-old SPF chicks, were divided into SE group (30 chicks), mortality group (10 chicks), both orally inoculated (1.

Multiclonal human origin and global expansion of an endemic bacterial pathogen of livestock.

Most new pathogens of humans and animals arise via switching events from distinct host species. However, our understanding of the evolutionary and ecological drivers of successful host adaptation, expansion, and dissemination are limited. is a major bacterial pathogen of humans and a leading cause of mastitis in dairy cows worldwide.

Multi-Drug Resistant Pathogenic Isolated from Wild Birds, Chicken, and the Environment in Malaysia.

Transmission of pathogenic microorganisms in the last decades has been considered a significant health hazard and pathogenic , particularly antibiotic-resistant strains, have long been identified as a zoonotic problem. This study aimed to investigate multidrug resistant pathogenic isolates from wild birds, chickens, and environment in selected Orang Asli and Malay villages in Peninsular Malaysia. The bacteriological culture-based technique, disc diffusion method, and multiplex Polymerase Chain Reaction (mPCR) assay was used to determine the occurrence of pathogenic strains in the several samples in the study.

On-Farm Practices Associated with Multi-Drug-Resistant and Derived from Cultured Fish.

Aquaculture activities have been implicated as responsible for the emergence of antimicrobial resistance (AMR), leading to broad dissemination and transference of antibiotic resistance to pathogens that affect humans and animals. The current study investigates the on-farm practices and environmental risk factors that can potentially drive the development and emergence of multi-drug-resistant (MDR) and in the aquaculture system. A cross-sectional study was conducted on 19 red hybrid tilapia ( spp.

Occurrence of antibiotic resistant and in wild birds, chickens, humans, and the environment in Malay villages, Kedah, Malaysia.

Foodborne pathogens have become a major concern not only due to the diseases they cause, but also because of the rise of antibiotic resistant strains in human and animals. The purposes of this study were to determine the occurrence of  and and their antibiotic resistance profiles in wild birds, chickens, humans, and the environment in Malay villages in Malaysia. Three Malay villages in Kota Setar, Kedah were chosen.

Prevalence and Antimicrobial Resistance of , and Derived from Farm-Raised Red Hybrid Tilapia ( spp.) and Asian Sea Bass (, Bloch 1970) on the West Coast of Peninsular Malaysia.

Antibiotics are widely used in intensive fish farming, which in turn increases the emergence of antimicrobial-resistant (AMR) bacteria in the aquatic environment. The current study investigates the prevalence and determines the antimicrobial susceptibility of , , and in farmed fishes on the west coast of Peninsular Malaysia. Over a period of 12 months, 32 aquaculture farms from the Malaysian states of Selangor, Negeri Sembilan, Melaka, and Perak were sampled.

Virulence Gene Profile, Antimicrobial Resistance and Multilocus Sequence Typing of Subsp. Serovar Enteritidis from Chickens and Chicken Products.

This study was undertaken to determine the virulence, antimicrobial resistance and molecular subtypes of in the Central Region of Peninsular Malaysia. A total of 45 Enteritidis were detected from live chicken (cloacal swab), and chicken products (fresh and ready-to-eat meat) samples upon cultural isolation and serotyping. Similarly, an antimicrobial susceptibility test based on the Kirby Bauer disk diffusion method as well as antimicrobial resistance AMR genes, virulence determinants and multilocus sequence typing (MLST) typing were conducted after the Whole Genome Sequencing and analysis of the isolates.

Discerning the Antimicrobial Resistance, Virulence, and Phylogenetic Relatedness of Isolates Across the Human, Poultry, and Food Materials Sources in Malaysia.

subspecies serovar Enteritidis is one of the major foodborne zoonotic pathogens globally. It has significantly impacted human health and global trade. In this investigation, whole-genome sequencing was employed to determine the antimicrobial resistance (AMR) pattern of a collection of Enteritidis isolated from humans, poultry, and food sources.

Antibacterial effect of acidic ionized water on horse wounds bacterial isolates.

Background And Aim: Horse wounds can be easily infected with bacteria depending on the nature of its cause such as laceration, abrasion, or puncture as well as the nature of its environment. Various treatments are available in managing open wounds, including the usage of topical antibiotics and antiseptics. However, antibiotic resistance has been a major concern attributed with chronic wound infection.

Inactivation of Different Phage Types and Safety and Efficacy of Inactivated Products in Chicken.

This study was conducted to inactivate phage types (SE pt) and to determine the safety and efficacy of inactivated SE pt in chickens. SE pt 1, 3A, 6A, 7, and 35 were inactivated and inoculated (0.20 mL) in 124 chickens divided into 6 groups (CV1, CV3A, CV6A, CV7, CV35, and CV0 as a control).

Seroprevalence and Distribution of Leptospiral Serovars in Livestock (cattle, Goats, and Sheep) in Flood-prone Kelantan, Malaysia.

Introduction: Leptospirosis is a bacterial disease that affects both humans and animals, the occurrence of which increases markedly during and after heavy rainfall and flooding. The aim of this study was to determine the serological prevalence of leptospiral infection in livestock after a voluminous flood in 10 districts of the Malaysian state of Kelantan.

Material And Methods: In December 2014, Kelantan was hit by an extensive flood.

Isolation of Mycobacterium avium and other nontuberculous mycobacteria in chickens and captive birds in peninsular Malaysia.

Background: Mycobacterium avium complex (MAC) causes a chronic infectious in the birds known as avian mycobacteriosis. Almost all species of the birds are susceptible to MAC which consists of two closely related species of mycobacteria, that is, M. avium and M.