J Zhejiang Univ Sci B
January 2019
p-Nitrophenylphosphate (PNPP) is usually employed as the substrate for enzyme-linked immunosorbent assays. p-Nitrophenol (PNP), the product of PNPP, with the catalyst alkaline phosphatase (ALP), will passivate an electrode, which limits applications in electrochemical analysis. A novel anti-passivation ink used in the preparation of a graphene/ionic liquid/chitosan composited (rGO/IL/Chi) electrode is proposed to solve the problem.
View Article and Find Full Text PDFGuang Pu Xue Yu Guang Pu Fen Xi
October 2011
A biosensor based on surface plasmon resonance (SPR) was developed for rapid detection of Escherichia coli O157 : H7. BIACORE 3000 SPR instrument and a dextran-modified sensor chip (CM5) were used. After activation with EDC/NHS, anti-E.
View Article and Find Full Text PDFGuang Pu Xue Yu Guang Pu Fen Xi
October 2009
In the present paper, the capture efficiencies of four different proportions of E. coli O157 : H7 and immunomagnetic beads (IMB) (1 : 6.34, 1 : 12.
View Article and Find Full Text PDFGuang Pu Xue Yu Guang Pu Fen Xi
September 2009
Near-infrared (NIR) spectroscopy technique was applied directly to the detection of pesticide phoxim residues. A sample pretreatment method was introduced. Samples were mixed with silica gel.
View Article and Find Full Text PDFGuang Pu Xue Yu Guang Pu Fen Xi
February 2009
In the present paper, a quantitative linear model betweena series of concentrations of E. coliO157 : H7 and counts by BPCL ultra weak luminescence analyzer was built up. And the influences of four different buffers with the same pH (pH = 7.
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