Integrated Multiparametric High-Content Profiling of Endothelial Cells.

Endothelial cells (ECs) are widely heterogeneous at the cell level and serve different functions at the vessel and tissue levels. EC-forming colonies derived from induced pluripotent stem cells (iPSC-ECFCs) alongside models such as primary human umbilical vein ECs (HUVECs) are slowly becoming available for research with future applications in cell therapies, disease modeling, and drug discovery. We and others previously described high-content analysis approaches capturing unbiased morphology-based measurements coupled with immunofluorescence and used these for multidimensional reduction and population analysis.

Direct electrochemistry of Shewanella loihica PV-4 on gold nanoparticles-modified boron-doped diamond electrodes fabricated by layer-by-layer technique.

Microbial Fuel Cells (MFCs) are robust devices capable of taping biological energy, converting pollutants into electricity through renewable biomass. The fabrication of nanostructured electrodes with good bio- and electrochemical activity, play a profound role in promoting power generation of MFCs. Au nanoparticles (AuNPs)-modified Boron-Doped Diamond (BDD) electrodes are fabricated by layer-by-layer (LBL) self-assembly technique and used for the direct electrochemistry of Shewanella loihica PV-4 in an electrochemical cell.

Physicochemical characterization, photo-stability and cytotoxicity of coenzyme Q10-loading nanostructured lipid carrier.

Nanostructured lipid carrier (NLC) is prospective active ingredients delivery systems based on combinations of solid lipids and liquid oil. Aqueous dispersion of NLC was prepared via hot high pressure homogenization technique. The CoQ10-NLC aqueous dispersion was prepared in order to improve the water solubility, photo-stability of Coenzyme Q10 (CoQ10).

Competitive protein adsorption on biomaterial surface studied with reflectometric interference spectroscopy.

Reflectometry interference spectroscopy (RIfS) is known as a highly sensitive and robust technique for direct, label-free detection of the interaction of biomacromolecules in real time and in situ. The aim of the present study was to investigate the competitive protein adsorption on the surface of fluorocarbon end-capped poly(carbonate) urethane (PCUF) and polystyrene (PS) based on the RIfS method. The surface energy and microstructures of PCUF and PS were characterized by contact angle measurement and atomic force microscopy.

Protein arrays based on biotin-streptavidin system for the simultaneous detection of TORCH infections.

In this paper, we developed a protein array based on biotin-streptavidin system (PABS) used in the identification of IgM antibodies against TORCH antigens, including toxoplasma gondii (TOX), rubella virus (RuV), cytomegalovirus (CMV) and herpes simplex virus types II (HSV-2) antigens. The detection signal intensities and sensitivities between the PABS and the direct labeling array system (DLAS) were compared. The linear ranges of detectable IgM antibodies in PABS were 0.

Development of a fluorescent and colorimetric detection methods-based protein microarray for serodiagnosis of TORCH infections.

We developed a protein microarray methodology that has the ability of serodiagnosis of IgM antibodies directed against TORCH pathogens. Six chemical surface modifications were validated by a dimension atomic force microscope (AFM) and contact angle measurement, agarose modified surface of which offered an appropriate platform for detecting IgM antibody. Further, signal amplification sensitivities on agarose modified microarrays were detected by Cy3-labeled biotin-streptavidin and immunogold-based assays.

Construction of an antibody microarray based on agarose-coated slides.

The antibody microarray, a high-throughput multiplex immunoassay method, has become a significant tool for quantitative proteomics studies. We describe here the strategies for optimizing the condition of antibody microarray building based on agarose-coated slides. In this study, modified glass slides were robotically printed with capture antibodies against monocyte chemoattractant protein 1 (MCP-1), then dilutions of the cytokine were applied to the arrays, and the protein was detected with biotin-labeled antibody coupled with Cy3-conjugated streptavidin.

HeLa cell adhesion on various collagen-grafted surfaces.

Cell adhesion is important to develop cell microarrays and biocompatible materials. Collagen has been reported to be able to improve cell adhesion. In this paper, two collagen coating methods (collagen grafted directly on the substrate and chitosan-modified substrate) were carried out, on which the adhesive behaviors of HeLa cells were studied.

[Protein microarrays and their medical applications].

The microarrays have revolutionised biomedical experimentation and diagnostics, enabling ordered high throughput analysis. During the past decade, classic solid phase substrates, such as microlitre plates, membrane filters and microscopic slides, were turn into high-density, chip-like structure. The concept of the arrayed library was central to this development which now extends from DNA to protein.