Comparison of Chlorhexidine Gluconate, Sodium Hypochlorite, Neem Extract, and Microwave Radiation for Disinfection of Type IV Dental Stone.

Objective:  The present study evaluated the effect of chemical disinfectants and microwave sterilization on the removal of biofilm containing , , and from type IV dental stone.

Materials And Methods:  One hundred twenty-eight ( = 128) type IV dental cast stone specimens were prepared, and biofilms of microorganisms were cultured. Dental stone samples were subjected to disinfection protocols, including 0.

Enhancing the antibacterial and surface hardness of glass ionomer cement modified with and Chlorhexidine: An in vitro study.

Objective: This laboratory study evaluated the effect of () root extracts and Chlorhexidine Digluconate (CHX) on the antibacterial and surface hardness properties of glass ionomer cement (GIC).

Methods: The in vitro experimental study was conducted at the Baqai Institute of Pharmaceutical Sciences of Baqai Medical University, Karachi, Pakistan, from October 2022 to March 2023. There were a total four experimental groups.

A rare biofilm dispersion strategy demonstrated by Staphylococcus aureus under oxacillin stress.

Staphylococcus aureus (S. aureus), a versatile Gram-positive bacterium, is implicated in a spectrum of infections, and its resilience is often attributed to biofilm formation. This study investigates the effect of sub-inhibitory doses of oxacillin on biofilm formation by methicillin-resistant S.

Nanotubes Formation in .

The present study discusses a biofilm-positive isolate that survives at pH levels ranging from 4.0 to 9.0.

Biogenic Selenium Nanoparticles and Their Anticancer Effects Pertaining to Probiotic Bacteria-A Review.

Selenium nanoparticles (SeNPs) can be produced by biogenic, physical, and chemical processes. The physical and chemical processes have hazardous effects. However, biogenic synthesis (by microorganisms) is an eco-friendly and economical technique that is non-toxic to human and animal health.

Design and analysis of mRNA vaccine construct against .

infections are continuously growing. Causative serovars have gained enhanced drug resistance and virulence. Current vaccines have fallen short of providing sufficient protection.

Banned Sudan dyes in spices available at markets in Karachi, Pakistan.

Sudan dyes were investigated in branded and non-branded spices, commonly available in the markets of Karachi, Pakistan. High performance liquid chromatography (HPLC) with a variable wavelength detector (VWD) was applied to determine Sudan dyes I-IV. The non-branded samples had higher concentrations of Sudan dyes than the maximum limits of 0.

Biosynthesis of Selenium Nanoparticles (via BSN313), and Their Isolation, Characterization, and Bioactivities.

Among the trace elements, selenium (Se) has great demand as a health supplement. Compared to its other forms, selenium nanoparticles have minor toxicity, superior reactivity, and excellent bioavailability. The present study was conducted to produce selenium nanoparticles (SeNPs) via a biosynthetic approach using probiotic BSN313 in an economical and easy manner.

A Size-dependent Bioaccumulation of Metal Pollutants, Antibacterial and Antifungal Activities of Telescopium telescopium, Nerita albicilla and Lunella coronata.

We assessed metal/metalloid pollutants (through multi-indices) in seawater, sediments, tissues and shells of gastropods using various indices such as contamination degree (modified/unmodified; Cd/mCd; 1875/187.5). From sediment indices; e.

Correlation between antibiotic resistance and phylogenetic types among multidrug-resistant isolated from urinary tract infections.

Objectives: Emergence of multidrug resistance has reduced the choice of antimicrobial regimens for UTIs. To understand the association of phenotype and genotype among uropathogens.

Materials And Methods: Six hundred and twenty-eight (628) urine samples were collected and analyzed.

Study of class 1 integrons and plasmid profile among multiple drug resistant uropathogenic Escherichia coli.

The emergence of multidrug resistance in uropathogenic Escherichia coli (UPEC) is associated with the presence of drug resistant plasmids and integrons which facilitate horizontal gene transfer which impose serious challenges in patients with urinary tract infections (UTIs). The proposed research study is designed to determine emerging antibiotic resistance trends and the presence of plasmids and class 1 integron in UPEC. A total 74 strains of urinary pathogens were procured among them 50 UPEC isolates were selected and their antibiotic resistance pattern was performed by CLSI guidelines.

Evaluation of heavy metal pollutants in salt and seawater under the influence of the Lyari River and potential health risk assessment.

Heavy metals intoxication through edible salt poses serious health hazards. The conducted research assessed the levels of heavy metals, health risks of salt samples, and the pollution index of seawater obtained from saltpans located at Sandpit, Karachi, Pakistan, which receive untreated effluents through the Lyari River. Seawater (n = 27) and salt samples (n = 27) were prepared for the detection of Al, Cd, Pb, Cr, Fe, Cu, Hg, Ni, As and Zn through atomic absorption spectroscopy, mean concentrations ± S.

Mesenchymal Stem Cell Derived-Exosomes as Effective Factors in Reducing Cytokine Storm Symptoms of COVID-19.

Given that conventional therapies are ineffective for COVID-19, obtained exosomes from stem cells have been proposed as a sustainable and effective treatment. Exosomes are subsets with lengths between 30 and 100 nanometers, and they can be secreted by different cells. Exosomes are containing different types of miRNAs, mRNAs, and different proteins.

Surviving under pollution stress: Antibacterial and antifungal activities of the Oyster species (Magallana bilineata and Magallana cuttackensis).

Antibiotic effectively controls the bacterial and fungal infections. Pathogens developing multi-drug resistance are a global health concern, which necessitate developing new molecules to overcome the resistance dilemma. This study explores the intrinsic ability of marine oysters synthesizing bioactive compounds.

An effective weapon against biofilm consortia and small colony variants of MRSA.

Objectives: This study was designed to investigate the effect of AgNPs (10 nm and 30 nm) on different phenotypes of biofilm consortia.

Materials And Methods: A total of eighteen biofilm-producing isolates of Methicillin-Resistant (MRSA) were used in the present study. Tube methods, Congo-red agar method, and scanning electron microscopy (SEM) were used to study biofilm phenotypes.

Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli.

Biofilm is an important virulent marker attributed to the development of urinary tract infections (UTIs) by uropathogenic (UPEC). Drug-resistant and biofilm-producing UPEC are highly problematic causing catheter-associated or recurrent UTIs with significant morbidity and mortality. The aim of the current study was to investigate the prevalence of biofilm formation and phylogenetic groups in drug-resistant UPEC to predict their ability to cause disease.

Enhanced modulation of gut microbial dynamics affecting body weight in birds triggered by natural growth promoters administered in conventional feed.

This study explored the effects of natural growth promoters (phytogenic feed additives and organic acids) on animal performance, carcass characteristics, blood parameters, gut microflora composition, and microbe-host interactions in broiler chickens over a 42-day feeding period. Two-hundred-fifty-day-old chicks were randomly assigned to one of five treatments: (i) control diets (CON); (ii) control diets + 40 g/tons antibiotic growth promoter (AB); (iii) control diets + 3 kg/tons organic acids (ORG); (iv) control diets + 3 kg/tons phytogenic feed additives (PHY); (v) control diets + 3 kg/tons organic acids + phytogenic feed additive combination (COM). A non-significant differences ( > ) were observed in broiler performance among treatments at 21 days of age; however, a gradually increasing body weight gain and reduced feed conversion ratio were observed at 42 days in treatments versus control group.

Determination of antimicrobial effect of protamine by transmission electron microscopy and SDS PAGE on isolates from diabetic foot infection.

Objectives: Diabetic foot infection is one of the major complications of diabetes leading to lower limb amputations. Isolation and identification of bacteria causing diabetic foot infection, determination of antibiotic resistance, antimicrobial potential of protamine by electron microscopy and SDS-PAGE analysis, arethe aims of this study.

Materials And Methods: 285 pus samples from diabetic foot infection patients were collected from different hospitals of Karachi and Capital Health Hospital, Halifax, Canada.

Isolation of selenium-resistant bacteria and advancement under enrichment conditions for selected probiotic Bacillus subtilis (BSN313).

The main aim of this work was to screen, isolate, and identify a probiotic selenium (Se)-resistant strain of Bacillus subtilis, using the 16S rDNA sequencing approach and subsequently optimize conditions. Initially, conditions were enhanced in two univariate optimization environments: shakings flask and a bioreactor. After solving optimization for selected variables, conditions were further optimized using orthogonal array testing.

Study of class 1 integrons in multidrug-resistant uropathogenic isolated from different hospitals in Karachi.

Objectives: is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic , prevalence of the ESBL gene group and class 1 integrons.

Materials And Methods: Clinical isolates of uropathogenic were isolated from different hospitals of Karachi.

Relationship of cell surface hydrophobicity with biofilm formation and growth rate: A study on and .

Objectives: This study was designed to determine the relationship of and isolates in multispecies biofilms and their individual phenotypic characters in biofilm consortia.

Materials And Methods: The subject isolates were recovered from different food samples and identified on the basis of growth on differential and selective media. Tube methods, Congo-red agar method, and scanning electron microscopy (SEM) were used to study biofilms phenotypes.

Identification and growth optimization of a Marine Bacillus DK1-SA11 having potential of producing broad spectrum antimicrobial compounds.

Control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. Marine bacteria are an enormous source of bio-controlling agents. The aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain DK1-SA11 isolated from Qingdao Bay of China Yellow Sea.

Development, Validation and Application of RP-HPLC Method: Simultaneous Determination of Antihistamine and Preservatives with Paracetamol in Liquid Formulations and Human Serum.

In this article we describe development and validation of stability indicating, accurate, specific, precise and simple Ion-pairing RP-HPLC method for simultaneous determination of paracetamol and cetirizine HCl along with preservatives i.e. propylparaben, and methylparaben in pharmaceutical dosage forms of oral solution and in serum.