Application of neuroendoscopic surgical techniques in the assessment and treatment of cerebral ventricular infection.

Cerebral ventricular infection (CVI) is one of the most dangerous complications in neurosurgery because of its high mortality and disability rates. Few studies have examined the application of neuroendoscopic surgical techniques (NESTs) to assess and treat CVI. This multicenter, retrospective study was conducted using clinical data of 32 patients with CVI who were assessed and treated by NESTs in China.

Study on the expression levels of CXCR4, CXCL12, CD44, and CD147 and their potential correlation with invasive behaviors of pituitary adenomas.

Objective: To evaluate the factors of CXCR4, CXCL12, CD44, and CD147 as early potential diagnostic biomarkers by determining their expression levels in invasive and non-invasive pituitary adenomas.

Methods: Fresh pituitary adenoma specimens were collected from 35 pituitary adenoma (21 invasive and 14 non-invasive) patients who underwent surgical treatment in our Neurosurgery Department between January and April of 2009. The expression levels of CXCR4, CXCL12, CD44, and CD147 were evaluated firstly by flow cytometry, fluorescence microscopy in single cell suspensions, and then by immunohistochemical staining of paraffin tissue sections.

[Osmotic demyelination syndrome in patients with hyponatremia caused by neurologic disorders].

Hyponatremia is relatively common in patients with neurologic disorders, while its diagnosis and treatment remain controversial. Osmotic demyelination syndrome (ODS) has shown to be closely associated with hyponatremia. ODS patients often present as central pontine myelinolysis, extrapontine myelinolysis, or both.

[Clinical application of neuronavigation in transsphenoidal microsurgery of pituitary adenomas].

Objectives: To summarize the experiences in clinical application of neuronavigation in transsphenoidal microsurgery of specific pituitary adenomas, and to discuss its indications.

Methods: From January 2006 to December 2010, 138 cases of transsphenoidal microsurgery for specific pituitary adenomas under neuronavigation were reviewed. The indications for neuronavigation in transsphenoidal microsurgery includes: recurrent or regrowth of residual pituitary adenomas after former transsphenoidal surgery in 36 cases, invasive pituitary adenomas in 45 cases, extremely laterally or deeply situated microadenomas in 45 cases, poor pneumatization of the sphenoid in 4 cases, skull base anomalies due to osteodysplasia fibrosa in 3 cases, narrow space between bilateral internal carotid arteries in 4 cases, distortion of nasal septum in 1 case.

[Thyroid-stimulating hormone pituitary adenomas: clinical characteristics, diagnosis and treatment].

Objective: To study the clinical characteristics, diagnosis and surgical effects of thyroid-stimulating hormone pituitary adenomas (TSH-omas).

Methods: The clinical data of 19 patients (14 female and 5 male) with TSH-omas were analyzed retrospectively in this study from January 2001 to December 2008. The patients ranged from 20 to 70 years old (average 40.

[Effects of adrenalectomy on the treatment of Cushing disease].

Objective: To discuss the effects of adrenalectomy (ADX) on the treatment of Cushing's disease(CD).

Methods: Clinical data of 15 cases of CD between January 1980 and December 2005 were analyzed to evaluate operative indications, complications and the changes of hypercortisolism and hormone levels pre- and post- adrenalectomy.

Results: All the patients involved underwent transsphenoidal pituitary surgery previously.

[Transsphenoidal microsurgical results of non-invasive prolactinomas].

Objective: To analyze the transsphenoidal microsurgical results of non-invasive prolactinomas, in order to provide reference for their treatment choice.

Methods: To review the transsphenoidal microsurgical results of 234 non-invasive prolactinomas treated in our department in recent 10 years, and to analyze the prognostic factors. There were 18 males and 216 females, aged between 13 and 58 years, averaged (31.

Magnetic resonance imaging characteristics and surgical results of adrenocorticotropin-secreting pituitary adenomas.

Objective: To evaluate magnetic resonance imaging (MRI) characteristics and surgical results of adrenocorticotropin (ACTH)-secreting pituitary adenomas.

Methods: MRI characteristics and relationship between MRI positive rate and surgical results of 266 patients with pathologically confirmed Cushing's disease were analyzed retrospectively. All patients underwent thin-section sagittal and coronal scans of the pituitary gland before and after administration of gadolinium-diethylene triamine pentaacetic acid (Gd-DTPA) on a 1.

[Extended transsphenoidal operation for giant and invasive pituitary adenomas].

Objective: To evaluate the possibility of extended transsphenoidal approach for removing the giant and invasive pituitary adenomas.

Methods: The clinical data of 64 cases with giant and invasive pituitary adenoma treated by extended transsphenoidal approach were studied retrospectively.

Results: Among 64 patients, 51 had total resection and 13 had subtotal resection.

[Factors influencing the recurrence of Cushing's disease treated by transsphenoidal surgery].

Objective: To explore factors influencing the recurrence of patients with Cushing's disease after transsphenoidal surgery.

Methods: We retrospectively analyzed the clinical data of 182 patients treated by transsphenoidal surgery with Cushing's disease in our department in PUMC Hospital from 1992 to 2002.

Results: The recurrence rates were significantly different when patients had different radiological findings (P = 0.

[Evaluation of emergency craniotomy for the treatment of patients with intracranial metastases of choriocarcinoma].

Objective: To study the diagnosis, management and prognosis of intracranial metastases of gestational trophoblastic tumour (GTT) patients who had emergency surgical decompression due to highly increased intracranial pressure.

Methods: Ninety-three patients with intracranial metastases of gestational trophoblastic tumour were hospitalized in our department of Peking Union Medical College Hospital from 1985 to 2004. Thirteen of them who underwent emergency craniotomy were retrospectively analyzed, and all of them presented with raised intracranial pressure and intracerebral haemorrhagic mass.

[Relationship between invasive pituitary adenomas and cavernus sinus and cariod artery and surgical outcomes].

Objective: To analyze the relationship between invasive pituitary adenomas and cavernus sinus and cariod artery and to predict their surgical outcomes.

Methods: Totally 270 patients with pituitary tumors were investigated in this retrospective study, including 113 men and 157 women, with a mean age of 40.8 years.

[Extended transsphenoidal approach to giant tumors in sellar and clival area].

Objective: To explore the effects of extended transsphenoidal approach, a new approach for removing giant tumors in sellar and clival area.

Methods: Twenty-eight patients, 19 males and 9 females, aged 17 - 67 with a mean age of 47.8, with a mean history of 6.

Elevated soluble epidermal growth factor receptor level in pituitary adenoma and carcinoma.

Objective: To investigate effect of the soluble epidermal growth factor receptor (sEGFR/sErbB1) level in the peripheral blood in development, invasiveness, apoplexy of each type of pituitary tumor.

Methods: The sEGFR level was determined in peripheral serum from 190 patients with pituitary diseases by enzyme linked immunosorbent assay. The sEGFR levels were measured in 10 pituitary Rathke's pouch, 18 pituitary hyperplasia, 161 pituitary adenomas including 30 microadenomas, 83 large adenomas, 48 giant adenomas, 1 pituitary carcinoma, and 28 healthy controls.

[Expressive level of vascular endothelial growth factor in peripheral blood in patients with pituitary adenomas].

Objective: To discuss whether vascular endothelial growth factor (VEGF) in the peripheral blood can reflect the biological activities of pituitary adenomas.

Methods: The concentrations of VEGF in peripheral blood were measured with ELISA in 203 patients with pituitary adenomas, 22 patients with pituitary hyperplasia, 7 patients with pituitary Rathke' pouch and 3 patients with pituitary abscess.

Results: The serum VEGF levels were (366.

[Measurement of soluble CD44v6 in peripheral blood as assistant diagnosis of invasive pituitary adenomas].

Objective: To investigate the value of measuring the concentration of soluble CD44 splice variant 6 (sCD44v6) in peripheral blood in patients with invasive and non-invasive pituitary adenomas.

Methods: The concentrations of sCD44v6 in peripheral blood were measured with ELISA in 68 patients with invasive pituitary adenomas and 100 patients with non-invasive pituitary adenomas.

Results: The serum concentration of sCD44v6 in patients with invasive pituitary adenomas was lower than that in patients with non-invasive pituitary adenomas, while the latter was lower than that in healthy controls.

[Production and purification of helper free recombinant adeno-associated virus 2].

Objective: To explore an efficient and simple way to product and purify helper free adeno-associated virus vectors.

Method: Helper free rAAV system was transferred into HEK293T cells through phosphorated calcium method, thus producing rAAV, then the rAAV vector was purified through chloroform-PEG8000/NaCl-chloroform method and ultrafiltration. SDS-PAGE protein electrophoresis and Western blotting were used to detect the rAAV protein.

[Hyponatremia after surgery of pituitary adenomas].

Objective: To clarify the frequency and presentation associated factors, pathogenetic mechanism, treatment and outcome of hyponatremia after surgery of pituitary adenoma.

Methods: Retrospectively reviewed the data of 186 patients who underwent pituitary surgery between January 1999 and June 2000 in the department.

Results: 72 (38.

[Diagnosis and treatment of sellar germinoma].

That eleven patients with sellar germinomas were confirmed by pathological examinations were retrospectively reviewed. In the 11 patients, 2 were males and 9 were females. Mean age was 16.

[Gene therapy of rat prolactinomas mediated by adenoviral vectors with rat tyrosine hydroxylase gene].

Objective: To investigate the potential of gene therapy of rat prolactinomas mediated by adenoviral vectors with a gene encoding rat tyrosine hydroxylase.

Methods: Recombinant replication-deficient adenovirus named Ad-GFP-TH with rat TH-cDNA and control adenovirus named Ad-GFP were constructed by homologous recombination in bacterial cells. The rat pituitary prolactinoma cell line MMQ are chosen as the target cells to study the effect of gene therapy on their growth and prolactin secretion mediated by Ad-GFP-TH.

[Intra-arterial thrombolysis in patients with acute ischemic stroke of the cervical internal carotid artery occlusive disease].

Objective: To observe the safety and efficacy of local intra-arterial thrombolysis (LIT) in patients with acute ischemic stroke of cervical internal carotid artery occlusive disease.

Methods: 10 patients were treated by LIT. 2 of 10 patients were further treated by either angioplasty or endarterectomy.

[Serial analysis of gene expression in the pituitary adenomas and para-tumor normal pituitary tissues].

Objective: To observe the characteristics and difference of gene expression in the pituitary adenomas and para-tumor normal pituitary tissues.

Methods: Using serial analysis of gene expression (SAGE), two SAGE libraries were generated. Forty clones from each SAGE library were sequenced, and the results were analyzed by SAGE2000 software and compared with the SAGE map at NCBI.

[Measurement of epidermal growth factor receptor concentration in the pre- and postoperative serum in patients with meningiomas].

Objective: To investigate effect of the serum concentration of epidermal growth factor receptor (EGFR) in the pre- and postoperative peripheral blood in patients with meningiomas.

Methods: Using ELISA, the EGFR concentration were measured in the pre- and postoperative serum in 53 patients with meningiomas. In this study, the patients were divided into preoperative group, postoperative group (including 42 total resection, 11 subtotal resection), and 28 healthy control.