Association of HIF-1α and NDRG2 Expression with EMT in Gastric Cancer Tissues.

Objective: This study aims to investigate the differences in the expression of hypoxia-inducible factor-1α (HIF-1α), N-myc downstream-regulated gene 2 (NDRG2) and epithelial mesenchymal transition (EMT)-related proteins in normal gastric tissues, gastric cancer tissues and lymph node metastasis.

Methods: Immunohistochemistry was used to detect the expression of HIF-1α, NDRG2, E-cadherin, Snail and Twist in normal gastric tissues, gastric cancer tissues and lymph node metastasis.

Results: In normal gastric tissues, HIF-1α was not expressed, NDRG2 was highly expressed.

Phthalazino[1,2-b]quinazolinones as p53 Activators: Cell Cycle Arrest, Apoptotic Response and Bak-Bcl-xl Complex Reorganization in Bladder Cancer Cells.

p53 inactivation is a clinically defined characteristic for cancer treatment-nonresponsiveness. It is therefore highly desirable to develop anticancer agents by restoring p53 function.1 Herein the synthesized phthalazino[1,2-b]quinazolinones were discovered as p53 activators in bladder cancer cells.

Arylurea Derivatives: A Class of Potential Cancer Targeting Agents.

Arylurea derivatives, an important class of small molecules, have received considerable attention in recent years due to their wide range of biological applications. Various molecular targeted agents with arylurea scaffold as potential enzyme/receptor inhibitors were constructed with the successful development of sorafenib and regorafenib. This review focuses on those arylureas possessing anti-cancer activities from 2010 to date.

[Expression of cytochrome P450scc in human early placenta].

The present study has determined the cellular distribution of cytochrome P450scc in human early placenta by immunohistochemistry and assessed the abundances of cytochrome P450scc protein in the villous tissue at 6-9 weeks of human pregnancy by Western blotting. The results showed that immunoreactive P450scc was mainly localized in the villous syncytiotrophoblast cells but not in the cytotrophoblast cells and the villous core, and that the expression of protein for cytochrome P450scc in human early placental villous tissues increased gradually with advancing weeks of pregnancy. Taken together, these findings suggest that the syncytiotrophoblast cells are the major site expressing P450scc in human early placenta, and that increasing expression of cytochrome P450scc in placental villi might establish a foundation, in terms of enzymology, for site-shift of progesterone biosynthesis from the corpus luteum to the placenta during human early pregnancy.