Nitrite is a typical food additive and preservative used in the food industry, which has attracted considerable attention due to its severe adverse effects on human health. Herein, a sensitive and highly selective ratiometric colorimetric sensing platform for the detection of nitrite was created based on a polymetallic oxide nanozyme, CoMnO nanofibers (CMO) catalysis integrated with the particular diazotization reaction. The nanozyme has superior oxidase-like activity ( was 0.
View Article and Find Full Text PDFOrganophosphorus pesticides (OPs) are widely used in agricultural production, but their residues could cause pollution to the environment and living organisms. In this paper, a simple dual-readout method for OPs detection was proposed based on ChOx single enzyme inhibition. Firstly, ChOx can catalyze the production of HO from choline chloride (Ch-Cl).
View Article and Find Full Text PDFIn this study, we have developed a sensitive approach to measure organophosphorus pesticides (OPs) using graphitic-phase CN nanosheets (g-CN) combined with a nanomaterial-based quencher, MnO nanosheets (MnO NS). Since MnO NS can quench the fluorescence of g-CN via the inner-filter effect (IFE), enzymatic hydrolysate (thiocholine, TCh) can efficiently trigger the decomposition of MnO nanosheets in the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCh), resulting in the fluorescence recovery of g-CN. OPs, as inhibitors to AChE activity, can prevent the generation of TCh and decomposition of MnO nanosheets while exhibiting fluorescence quenching.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
March 2022
A colorimetric gold nanoparticles (AuNPs)-based acetylcholinesterase (AChE) assay was designed for the first time to measure the concentration of parathion-methyl (PM) in lake water samples. In this assay, the analyte PM inhibited the hydrolysis of acetylthiocholine (ATCh) by AChE, preventing the formation of thiocholine (TCh) that would otherwise react with the AuNPs catalyst and deactivate the catalyst. Therefore, in the presence of PM, the AuNPs catalyzed the oxidation of the 3,3',5,5'-tetramethylbenzidine (TMB) colorimetric indicator to oxTMB, inducing a visual color change from colorless to blue.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
February 2021
In this paper, a novel fluorescence assay has been constructed for the determination of parathion-methyl (PM) by using 4-amino-3-hydroxy-1-naphthalenesulfonic acid (AHNSA) as probe. MnO nanosheets (MnO NS) could quench the fluorescence of AHNSA, while Mn, the reduction product of MnO NS, has no influence on it, resulting in fluorescence recovery. This is because that MnO NS have oxidized characteristic, and they can react with choline (TCh), which is the product of acetylthiocholine (ATCh) catalyzed by acetylcholinesterase (AChE).
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