Tetramethylpyrazine attenuates the cancer stem cell like-properties and doxorubicin resistance by targeting HMGCR in breast cancer.

Background: Tetramethylpyrazine (TMP), a key bioactive constituent derived from Ligusticum wallichii Franchat, has demonstrated efficacy in mitigating multidrug resistance (MDR) in human breast cancer (BC) cells. However, the precise mechanisms underlying its action remain poorly understood.

Purpose: Cancer stem cells (CSCs) are widely recognized as the primary contributors to MDR.

PlasGO: enhancing GO-based function prediction for plasmid-encoded proteins based on genetic structure.

Background: Plasmid, as a mobile genetic element, plays a pivotal role in facilitating the transfer of traits, such as antimicrobial resistance, among the bacterial community. Annotating plasmid-encoded proteins with the widely used Gene Ontology (GO) vocabulary is a fundamental step in various tasks, including plasmid mobility classification. However, GO prediction for plasmid-encoded proteins faces 2 major challenges: the high diversity of functions and the limited availability of high-quality GO annotations.

Baihui-Penetrating-Qubin Acupuncture Attenuates Neurological Deficits Through SIRT1/FOXO1 Reducing Oxidative Stress and Neuronal Apoptosis in Intracerebral Hemorrhage Rats.

Background: Intracerebral hemorrhage (ICH) is a significant global disease with high mortality and disability. As of now, there is no effective therapy available. Oxidative stress and neuronal apoptosis play essential roles in ICH, determining neuronal survival.

Association between atherogenic index of plasma and post-stroke depression: a cross-sectional study.

Few studies have established a link between the atherogenic index of plasma (AIP) and post-stroke depression (PSD). This study aims to further investigate the potential relationship between the AIP and PSD, and to provide references for the prevention and prognosis management of patients with PSD. A cross-sectional study was conducted using data from participants in the National Health and Nutrition Examination Survey from 2005 to 2018.

Suppression of cGAS/STING pathway-triggered necroptosis in the hippocampus relates HS to attenuate cognitive dysfunction of Parkinson's disease.

Background: Cognitive dysfunction is the most severe non-motor symptom of Parkinson's disease (PD). Our previous study revealed that hydrogen sulfide (HS) ameliorates cognitive dysfunction in PD, but the underlying mechanisms remain unclear. Hippocampal necroptosis plays a vital role in cognitive dysfunction, while the cGAS/STING pathway triggers necroptosis.

Impoundment-induced stoichiometric imbalance exacerbated phosphorus limitation in a deep subtropical reservoir: Implications for eutrophication management.

Impoundments play a vital role as nutrient sinks, capable of retaining and exporting nitrogen (N) and phosphorus (P) at different rates. The imbalance in N and P stoichiometry relative to phytoplankton demand often determines the limiting nutrient of phytoplankton biomass in these systems. This critical factor has a substantial impact on the management of eutrophication, encompassing the formulation of nutrient control strategies and the setting of regulatory thresholds.

Tracheloside, the main constituent of the total lignan extract from Trachelospermi Caulis, inhibited rheumatoid arthritis via IL-17/MAPK signaling pathway.

A quantitative analysis method was established using high-performance liquid chromatography (HPLC) to simultaneously determine five lignans in the total lignan extract (TLE) from Trachelospermi Caulis at 280 nm, including nortracheloside, tracheloside, nortrachelogenin, trachelogenin, and arctigenin. The method demonstrated good linearity for each lignan within their respective ranges, with precision, stability, and repeatability meeting the criteria for quantitative analysis. Tracheloside, the most abundant compound in the TLE, effectively inhibited the release of inflammatory factors IL-6 and IL-17, and suppressed the migration MH7A cells in vitro.

Functional Divergence of the Closely Related Genes PhARF5 and PhARF19a in Flower Formation and Hormone Signaling.

The ARF gene family plays a vital role in regulating multiple aspects of plant growth and development. However, detailed research on the role of the ARF family in regulating flower development in petunia and other plants remains limited. This study investigates the distinct roles of and in flower development.

MoS Nanosheets at Low Doses Induced Cardiotoxicity in Developing Zebrafish via Ferroptosis: Influence of Lateral Size and Surface Modification.

The widespread applications of molybdenum disulfide (MoS) nanosheets inevitably result in their release into aquatic environments, necessitating an exploration of their potential toxic effects on aquatic organisms. This study analyzes the cardiac responses of zebrafish larvae exposed to MoS, with a focus on the influence of size and surface modifications. At higher concentrations (1 and 5 mg/L), MoS nanosheets hampered larval growth without influencing cardiomyogenesis.

Ultrabroadband coherent perfect absorption with composite graphene metasurfaces.

We investigate the design and performance of a new multilayer graphene metasurface for achieving ultrabroadband coherent perfect absorption (CPA) in the THz regime. The proposed structure comprises three graphene patterned metasurfaces separated by thin dielectric spacer layers. The top and bottom metasurfaces have crossed shape unit cells of varying sizes, while the middle graphene metasurface is square-shaped.

Rhythmic states and first-order phase transitions in adaptive coupled three-dimensional limit-cycle oscillators.

This paper reports a phase transition in coupled three-dimensional limit-cycle oscillators with an adaptive coupling. We reveal that the multiple-cluster rhythmic states emerge when natural frequencies of oscillators follow uniform distribution (case I), but disappear for Gaussian distribution (case II). Furthermore, as the coupling strength K increases, two first-order phase transitions occur sequentially.

Capsular polysaccharide structure of Acinetobacter baumannii K58 from clinical isolate MRSN31468.

Capsular polysaccharides (CPS) of Acinetobacter baumannii is a virulence factor with diverse structures. CPS are produced by the CPS biosynthesis gene cluster in their K locus (KL). However, CPS variations may occur due to insertion of additional genes from external sources, e.

Hypertensive Nephropathy Changes the Expression of Drug-Metabolizing Enzymes and Transporters in Spontaneously Hypertensive Rat Liver and Kidney.

Background And Objectives: Hypertensive nephropathy (HN) has become one of the main causes of end-stage renal disease. Drug combination therapy is a common clinical treatment for HN. However, the impact of HN on drug-metabolizing enzymes and transporters, which may lead to drug-drug interactions (DDIs) and even trigger toxic side effects, remains unclear.

Inhibition of SGLT2 protects podocytes in diabetic kidney disease by rebalancing mitochondria-associated endoplasmic reticulum membranes.

Background: Sodium-glucose cotransporter 2 (SGLT2) inhibitors have changed the therapeutic landscape for diabetic kidney disease (DKD) patients, but their underlying mechanisms are complicated and not fully understood. Mitochondria-associated endoplasmic reticulum membranes (MAMs), the dynamic contact sites between mitochondria and the endoplasmic reticulum (ER), serve as intracellular platforms important for regulating cellular fate and function. This study explored the roles and mechanisms of SGLT2 inhibitors in regulating MAMs formation in diabetic podocytes.

Endocytosis restricts dendrite branching via removing ectopically localized branching ligands.

Neurons often grow highly branched and cell-type specific dendrite morphologies to receive and integrate information, which is the basis of precise neural circuit formation. Previous studies have identified numerous mechanisms that promote dendrite branching. In contrast, it is much less understood how this process is negatively regulated.

Enlarged choroid plexus related to atrophy of hippocampal subfield volumes and glymphatic dysfunction in benzodiazepine use disorder.

Aims: The aim of this study was to explore the relationship of choroid plexus (CP) volume with hippocampal subregion volume and glymphatic system in patients with benzodiazepine use disorders (BUD).

Methods: Eighty-four participants were recruited including 23 patients with BUD, 29 patients with chronic insomnia (CI) and 32 healthy controls (HC). The morphological alterations in CP, hippocampal subfield volumes and diffusion tensor image analysis along the perivascular space (DTI-ALPS) index were compared between the three groups and the relationships between CP volume and hippocampal subfield volumes and the glymphatic system were further investigated.

Facile synthesis and biomimetic amine-functionalization of chitosan foam for CO capture.

A high-performance biomass-based adsorption materials could be the promising trend for CO capture and storage technology. However, the direct application of biomass-based porous materials as a CO adsorbent with enhanced performance is an emerging issue. Herein, a facile synthesis and a biomimetic strategy were combined to prepare amine-functionalized chitosan foam for CO capture, and then a porous biomass is achieved for the application on the environment protection field.

Impacts of P4-ATPase Deletion on Membrane Asymmetry and Disease Development.

Phospholipids exhibit an asymmetrical distribution on the cell membrane. P4-ATPases, type IV lipid flippases, are responsible for establishing and maintaining this phospholipid compositional asymmetry. The essential β subunit CDC50 (also known as TMEM30) assists in the transport and proper functioning of P4-ATPases.

Dual Frequency-Regulated Magnetic Vortex Nanorobots Empower Nattokinase for Focalized Microvascular Thrombolysis.

Magnetic nanorobots are emerging players in thrombolytic therapy due to their noninvasive remote actuation and drug loading capabilities. Although the nanorobots with a size under 100 nm are ideal to apply in microvascular systems, the propulsion performance of nanorobots is inevitably compromised due to the limited response to magnetic fields. Here, we demonstrate a nattokinase-loaded magnetic vortex nanorobot (NK-MNR) with an average size around 70 nm and high saturation magnetization for mechanical propelling and thermal responsive thrombolysis under a magnetic field with dual frequencies.

Hyperbaric oxygen therapy enhances restoration of physical functional in patients with recurrent glioma: A case report.

Patients with recurrent glioblastoma often opt for hypofractionated stereotactic radiosurgery, which can cause various adverse reactions. The pharmacological interventions used to manage these adverse reactions are usually unsatisfactory. The present study reports the case of a patient with recurrent glioblastoma who underwent hyperbaric oxygen therapy followed by immediate hypofractionated stereotactic radiosurgery.

MONITTR allows real-time imaging of transcription and endogenous proteins in C. elegans.

Maximizing cell survival under stress requires rapid and transient adjustments of RNA and protein synthesis. However, capturing these dynamic changes at both single-cell level and across an organism has been challenging. Here, we developed a system named MONITTR (MS2-embedded mCherry-based monitoring of transcription) for real-time simultaneous measurement of nascent transcripts and endogenous protein levels in C.

Treatment of sludge hydrothermal carbonization wastewater by ferrous/sodium percarbonate system: Effect of wastewater composition and role of coagulation and oxidation.

It is crucial to explore the effect of complex wastewater compositions on the ferrous/sodium percarbonate (Fe(Ⅱ)/SPC) system and the role of oxidation-coagulation in designing water treatment processes. This study employed redundancy analysis to investigate the effects of wastewater constituents on oxidation and coagulation. Raman analysis, X-ray Photoelectron Spectroscopy, and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry were used to determine the roles of oxidation and coagulation in the system.