[Results of the first All-Russian consensus conference on bariatric surgery].

Until now, there has not been organized consensus for standardization in bariatric surgery In Russia. We present the results of the first Bariatric Surgery Consensus Conference conducted in Barnaul (March, 2023). A list of questions was proposed within 6 blocks: 1) general issues of bariatric surgery, 2) sleeve gastrectomy, 3) one-anastomosis gastric bypass («mini-gastric bypass»), 4) Roux-en-Y Gastric Bypass, 5) Single Anastomosis Duodenal Switch and other options for biliopancreatic bypass, 6) rare procedures.

Genomic and Transcriptomic Analysis of Pea ( L.) Breeding Line 'Triumph' with High Symbiotic Responsivity.

Pea ( L.), like most legumes, forms mutualistic symbioses with nodule bacteria and arbuscular mycorrhizal (AM) fungi. The positive effect of inoculation is partially determined by the plant genotype; thus, pea varieties with high and low symbiotic responsivity have been described, but the molecular genetic basis of this trait remains unknown.

T5-like phage BF23 evades host-mediated DNA restriction and methylation.

Bacteriophage BF23 is a close relative of phage T5, a prototypical that infects . BF23 was isolated in the middle of the XXth century and was extensively studied as a model object. Like T5, BF23 carries long ∼9.

Identification and Expression Analysis of the Family in L.

The (CEP) peptides play crucial roles in plant growth and response to environmental factors. These peptides were characterized as positive regulators of symbiotic nodule development in legume plants. However, little is known about the CEP peptide family in pea.

[Editing of Phage Genomes - Recombineering-Assisted SpCas9 Modification of Model Coliphages T7, T5, and T3].

Bacteriophages-viruses that infect bacterial cells - are the most abundant biological entities on Earth. The use of phages in fundamental research and industry requires tools for precise manipulation of their genomes. Yet, compared to bacterial genome engineering, modification of phage genomes is challenging because of the lack of selective markers and thus requires laborious screenings of recombinant/mutated phage variants.

A variable gene family encoding nodule-specific cysteine-rich peptides in pea ( L.).

Various legume plants form root nodules in which symbiotic bacteria (rhizobia) fix atmospheric nitrogen after differentiation into a symbiotic form named bacteroids. In some legume species, bacteroid differentiation is promoted by defensin-like nodule-specific cysteine-rich (NCR) peptides. NCR peptides have best been studied in the model legume Gaertn.

DNA Methylation Patterns Differ between Free-Living RCAM1026 and Bacteroids Formed in Symbiosis with Pea ( L.).

() is a common name for several genospecies of rhizobia able to form nitrogen-fixing nodules on the roots of pea ( L.) while undergoing terminal differentiation into a symbiotic form called bacteroids. In this work, we used Oxford Nanopore sequencing to analyze the genome methylation states of the free-living and differentiated forms of the strain RCAM1026.

Transposons-Based Clonal Diversity in Trematode Involves Parts of CR1 (LINE) in Eu- and Heterochromatin.

Trematode parthenitae have long been believed to form clonal populations, but clonal diversity has been discovered in this asexual stage of the lifecycle. Clonal polymorphism in the model species has been previously described, but the source of this phenomenon remains unknown. In this work, we traced cercarial clonal diversity using a simplified amplified fragment length polymorphism (SAFLP) method and characterised the nature of fragments in diverse electrophoretic bands.

[Organizing the medical care for the COVID-19 patients in non-infectious Moscow hospital: reassignment experience].

Aim: To present the results of work of National Medical Research Center of Treatment and Rehabilitation, reassigned for COVID-19 patients treatment during pandemic. Run-up methodology, procedures and working process organization are detailed.

Materials And Methods: 354 COVID-19 patients were treated from 13.

Endoscopic ultrasonography for intra-abdominal textiloma.

Introduction: The term textiloma is used to describe foreign body (swab) forgotten during surgery. We don't know any case of the description of endoscopic ultrasonography (EUS) for intra-abdominal textiloma.

Objectives: To determine the EUS capabilities in the topical diagnosis of intra-abdominal textiloma.

[Apparatus for electrophoresis on cellulose acetate films].

Type EFPA-30 apparatus for electrophoresis on cellulose acetate films was developed at the Specialized Design Office for Biophysical Equipment of the Moscow Research and Production Association BIOFIZPRIBOR. Three autonomic separation cameras are united in one block, that permits separation of 12, 24, and 36 samples; the apparatus is supplied with time transducer, acoustic and visual indication. Clinical trials of the apparatus were carried out at the Institute of Biological and Medical Chemistry of the USSR Academy of Medical Sciences, its commercial production is to be started.

[Use of a conductometric detector in capillary isotachophoresis of blood serum proteins].

Conductometric detectors can be employed for analysis of the blood serum proteins. Conductometric curves of various sera show at least 12 peaks corresponding to certain protein fractions and inorganic components. Isotachophoresis of individual protein preparations detects impurities in them.

[Storage and reconstruction of hybridomas producing monoclonal antibodies against viral antigens].

The conditions were optimized for freezing storage, restoring and further cultivation of hybridoma cells producing antibodies to viral antigens. The effect of density of cellular suspension frozen,concentration of calf embryo serum in cryoprotected medium and mild conditions of the restoring of hybridoma were studied. To restore deeply frozen hybridoma 24 hole plastic panels with a layer of feeding cells of the HEPES and insulin containing medium were used.