Publications by authors named "Zoran Z Gajic"
Recent massively-parallel approaches to decipher gene regulatory circuits have focused on the discovery of either -regulatory elements (CREs) or -acting factors. Here, we develop a scalable approach that pairs - and -regulatory CRISPR screens to systematically dissect how the key immune checkpoint is regulated. In human pancreatic ductal adenocarcinoma (PDAC) cells, we tile the locus using ∼25,000 CRISPR perturbations in constitutive and IFNγ-stimulated conditions.
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- - MultiPerturb-seq is a new CRISPR screening platform that combines single-nucleus chromatin accessibility, transcriptome analysis, and guide RNA capture to enhance the profiling of genetic information.
- - This high-throughput method utilizes advanced techniques like combinatorial indexing and droplet microfluidics to efficiently process and integrate multiple forms of data from single cells.
- - The research identified critical differentiation genes in a rare pediatric cancer, highlighting ZNHIT1 as a promising target for developing reprogramming therapies for cancer treatment.
Immune checkpoint blockade (ICB) has transformed the treatment of metastatic cancer but is hindered by variable response rates. A key unmet need is the identification of biomarkers that predict treatment response. To address this, we analyzed six whole exome sequencing cohorts with matched disease outcomes to identify genes and pathways predictive of ICB response.
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