Numerous studies have demonstrated that under low-velocity, low-energy impact conditions, although the surface damage to fiber-reinforced composite laminates may be minimal, significant internal damage can occur. Consequently, a progressive damage finite element model was specifically developed for thermoplastic carbon fiber-reinforced composite laminates subjected to low-speed impact loads, with the objective of analyzing the damage behavior of laminates under impacts of varying energy levels. The model utilizes a three-dimensional Hashin criterion for predicting intralayer damage initiation, with cohesive elements based on bilinear traction-separation law for predicting interlaminar delamination initiation, and incorporates a damage constitutive model based on equivalent displacement to characterize fiber damage evolution, along with the B-K criterion for interlaminar damage evolution.
View Article and Find Full Text PDFThe anterior pituitary plays a critical role in the endocrine system, contains gonadotrophs, which regulate reproductive efficiency by secreting follicle-stimulating hormone (FSH) and luteinizing hormone (LH). PPP2R2A is a serine-threonine phosphatase that regulates reproductive functions in both females and males, its function in pituitary cells remain unclear. Hu sheep is a highly prolific breed, which makes it suitable for studying reproductive mechanisms.
View Article and Find Full Text PDFAs a dominant mycotoxin, zearalenone (ZEA) has attracted extensive attention due to its estrogen-like effect and oxidative stress damage in cells. In order to find a way to relieve cell oxidative stress damage caused by ZEA, we treated goat granulosa cells (GCs) with ZEA and did a whole transcriptome sequencing. The results showed that the expression level of Sesterin2 (SESN2) was promoted extremely significantly in the ZEA group (p < .
View Article and Find Full Text PDFHT-2 toxin is a mycotoxin commonly found in food and water that can have adverse effects on male reproductive systems, including testosterone secretion. Ferroptosis and apoptosis are two types of programmed cell death that have been implicated in the regulation of cellular functions. Melatonin, a powerful antioxidant with various physiological functions, has been shown to regulate testosterone secretion.
View Article and Find Full Text PDFIntroduction: This study aims to investigate the long-term effects of spirulina supplementation in a high-fat diet (HFD) on rumen morphology, rumen fermentation, and the composition of rumen microbiota in lambs. Spirulina is a blue-green microalgae that has been shown to have high nutritional value for livestock.
Methods: Fifty-four lambs were randomly divided into three groups: a normal chow diet (NCD) group, a high-fat diet (HFD) group, and a high-fat diet supplemented with 3% spirulina (HFD+S) group.
Zearalenone (ZEA), also known as F-2 toxin, is a mycotoxin. Despite numerous reports of ZEA impairing livestock production performance and fertility, little information is available, including information about the mechanism underlying damage to cell metal ion transport. Copper, which is essential for cell survival as a metal ion, can consist of a variety of enzymes that facilitate abundant metabolic processes.
View Article and Find Full Text PDFMale germ cells directly affect the reproduction of males; however, their accurate isolation and culture in vitro is extremely challenging, hindering the study of germ cell development and function. CRISPR/dcas9, as an efficient gene reprogramming system, has been verified to promote the transdifferentiation of pluripotent stem cells into male germ cells by editing target genes. In our research, we explored the expression pattern of the germ cell related genes , and in Hu sheep testicular development and constructed the overexpression model using the CRISPR/dcas9 system.
View Article and Find Full Text PDFDNA methylation plays an important role in biological processes by affecting gene expression. However, how DNA methylation regulates phenotypic variation in Hu sheep remains unclear. Therefore, we generated genome-wide DNA methylation and transcriptomic profiles in the ovaries of Hu sheep with different prolificacies and genotypes (FecBB and FecB+).
View Article and Find Full Text PDFCarbon is the crucial source of energy during aerobic composting. There are few studies that explore carbon preservation by inoculation with microbial agents during goat manure composting. Hence, this study inoculated three proportions of microbial agents to investigate the preservation of carbon during goat manure composting.
View Article and Find Full Text PDFBiochim Biophys Acta Gene Regul Mech
September 2020
Long non-coding RNAs (lncRNAs) play an important regulatory role in mammalian fecundity. Currently, most studies are primarily concentrated on ovarian lncRNAs, ignoring the influence of uterine lncRNAs on the fecundity of female sheep. In this study, we found a higher density of uterine glands and endometrial microvessel density (MVD) in high prolificacy group of Hu sheep compared to low prolificacy groups (p < 0.
View Article and Find Full Text PDFThis study was conducted to investigate the effects of feeding regimens and dietary Trp levels on protein metabolism and regulation of the related gene expression in Yangzhou goslings. A 2 × 3 factorial completely randomized experiment was applied, and the treatments were designed as 2 feeding regimens (ad libitum vs. restricted feeding), and each contained 3 levels of Trp (low-Trp group, 0.
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