Protecting monoclonal antibodies via competitive interfacial adsorption of nonionic surfactants.

Hypothesis: Bioengineered monoclonal antibodies (mAbs) have gained significant recognition as medical therapies. However, during processing, storage and use, mAbs are susceptible to interfacial adsorption and desorption, leading to structural deformation and aggregation, and undermining their bioactivity. To suppress antibody surface adsorption, nonionic surfactants are commonly used in formulation.

Ultrasound Lung Aeration Map via Physics-Aware Neural Operators.

Lung ultrasound is a growing modality in clinics for diagnosing and monitoring acute and chronic lung diseases due to its low cost and accessibility. Lung ultrasound works by emitting diagnostic pulses, receiving pressure waves and converting them into radio frequency (RF) data, which are then processed into B-mode images with beamformers for radiologists to interpret. However, unlike conventional ultrasound for soft tissue anatomical imaging, lung ultrasound interpretation is complicated by complex reverberations from the pleural interface caused by the inability of ultrasound to penetrate air.

pH-Dependent Conformational Plasticity of Monoclonal Antibodies at the SiO/Water Interface: Insights from Neutron Reflectivity and Molecular Dynamics.

Investigating the molecular conformations of monoclonal antibodies (mAbs) adsorbed at the solid/liquid interface is crucial for understanding mAb solution stability and advancing the development of mAb-based biosensors. This study examines the pH-dependent conformational plasticity of a human IgG1k mAb, COE-3, at the SiO/water interface under varying pH conditions (pH 5.5 and 9).

A novel four-modal nano-sensor based on two-dimensional Mxenes and fully connected artificial neural networks for the highly sensitive and rapid detection of ochratoxin A.

Timely and accurate on-site detection of ochratoxin A (OTA) is extremely important for global public health. In this study, a fluorescence/colorimetric biosensor based on TiC nano-materials (TiC-NMS) and a machine-learning (ML) based fluorescence/colorimetric intelligent learning system for detection of OTA concentration (COTA) were developed. The sensor was fabricated by functionalizing TiC-NMS prepared by physical-exfoliation assisted metal-ion-induction using ssDNA.

Corneal nerve changes by anti-glaucoma medications examined by confocal microscopy.

Aim: To evaluate the effects of antiglaucoma eye drops on corneal nerves by confocal microscopy (IVCM).

Methods: This study comprised 79 patients diagnosed with glaucoma and 16 healthy control individuals. Among the glaucoma patients, 54 were treated with medication, while 25 remained untreated.

Directed differentiation of human embryonic stem cells into conjunctival epithelial cells.

Severe conjunctival damage can lead to extensive ocular cicatrisation, fornix shortening, and even ocular surface failure, resulting in significant vision impairment. Conjunctival reconstruction is the primary therapeutic strategy for these clinical conjunctival diseases. However, there have been limited studies on induced differentiation of conjunctival epithelial cells derived from stem cells.

Acoustic vibration promotes in vitro expansion of human embryonic stem cells.

Objectives: This study aimed to investigate the effect of acoustic vibration on the pluripotency of human embryonic stem cells (hESCs) and evaluate cell proliferation and self-renewal ability post-treatment.

Methods: The human ES cell line H1 was used for the experiments. hESCs were treated with an acoustic vibration device.

NAD supplementation improves mitochondrial functions and normalizes glaucomatous trabecular meshwork features.

Glaucoma is characterized by pathological elevation of intraocular pressure (IOP) due to dysfunctional trabecular meshwork (TM), which is the primary cause of irreversible vision loss. There are currently no effective treatment strategies for glaucoma. Mitochondrial function plays a crucial role in regulating IOP within the TM.

RAD21 deficiency drives corneal to scleral differentiation fate switching via upregulating WNT9B.

The cornea and sclera are distinct adjacent tissues, yet their stromal cells originate from common neural crest cells (NCCs). Sclerocornea is a disease characterized by an indistinguishable boundary between the cornea and sclera. Previously, we identified a mutation in a sclerocornea pedigree.

Nicotinamide promotes the differentiation of functional corneal endothelial cells from human embryonic stem cells.

Corneal transplantation represents the primary therapeutic approach for managing corneal endothelial dysfunction, but corneal donors remain scarce. Anterior chamber cell injection emerges as a highly promising alternative strategy for corneal transplantation, with pluripotent stem cells (PSC) demonstrating considerable potential as an optimal cell source. Nevertheless, only a few studies have explored the differentiation of functional corneal endothelial-like cells originating from PSC.

Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension.

High myopia is a risk factor for primary open angle glaucoma (POAG). The pathological mechanism of high myopia induced POAG occurrence is not fully understood. In this study, we successfully established the guinea pig model of ocular hypertension with high myopia, and demonstrated the susceptibility of high myopia for the occurrence of microbead-induced glaucoma compared with non-myopia group and the effect of YAP/TGF-β signaling pathway in TM pathogenesis induced by high myopia.

Mechanistic Insights into the Adsorption of Monoclonal Antibodies at the Water/Vapor Interface.

Monoclonal antibodies (mAbs) are active components of therapeutic formulations that interact with the water-vapor interface during manufacturing, storage, and administration. Surface adsorption has been demonstrated to mediate antibody aggregation, which leads to a loss of therapeutic efficacy. Controlling mAb adsorption at interfaces requires a deep understanding of the microscopic processes that lead to adsorption and identification of the protein regions that drive mAb surface activity.

AI-aided geometric design of anti-infection catheters.

Bacteria can swim upstream in a narrow tube and pose a clinical threat of urinary tract infection to patients implanted with catheters. Coatings and structured surfaces have been proposed to repel bacteria, but no such approach thoroughly addresses the contamination problem in catheters. Here, on the basis of the physical mechanism of upstream swimming, we propose a novel geometric design, optimized by an artificial intelligence model.

Structure and interaction of therapeutic proteins in solution: a combined simulation and experimental study.

The aggregation of therapeutic proteins in solution has attracted significant interest, driving efforts to understand the relationship between microscopic structural changes and protein-protein interactions determining aggregation processes in solution. Additionally, there is substantial interest in being able to predict aggregation based on protein structure as part of molecular developability assessments. Molecular Dynamics provides theoretical tools to complement experimental studies and to interrogate and identify the microscopic mechanisms determining aggregation.

A novel NbC MXene based aptasensor for rapid and sensitive multi-mode detection of AFB.

Rapid and accurate on-site detection of aflatoxin B (AFB) is of great significance for ensuring food safety. This work developed a dual mode aptasensor and a dual channel artificial neural network (ANN) intelligent sensor detection platform for simple and convenient quantitative detection of AFB in food. This sensor was prepared by encoding manganese ion (Mn) mediated surface concave niobium carbide MXene nanomaterials (NbC-MNs) using fluorescent group labeled aptamers (ssDNA-FAM).

Deep learning analysis on transmission electron microscope imaging of atomic defects in two-dimensional materials.

Defects are prevalent in two-dimensional (2D) materials due to thermal equilibrium and processing kinetics. The presence of various defect types can affect material properties significantly. With the development of the advanced transmission electron microscopy (TEM), the property-related structures could be investigated in multiple dimensions.

Artificial neural network-facilitated VC MNs-based colorimetric/fluorescence dual-channel biosensor for highly sensitive detection of AFB in peanut.

In this work, VC Mxene nano-enzyme materials (VC MNs) with excellent peroxidase-like activity and fluorescence quenching performance were prepared, and it was modified using 6-carboxyfluorescein-labelled aptamers (ssDNA-FAM) to construct a novel dual-mode sensor VC@ssDNA-FAM, with detection limits of 0.0477 ng mL and 0.2789 ng mL of fluorescence (linear range of 0.

Coupled Information-Epidemic Spreading Dynamics with Selective Mass Media.

As a pandemic emerges, information on epidemic prevention disseminates among the populace, and the propagation of that information interacts with the proliferation of the disease. Mass media serve a pivotal function in facilitating the dissemination of epidemic-related information. Investigating coupled information-epidemic dynamics, while accounting for the promotional effect of mass media in information dissemination, is of significant practical relevance.

Assessment of iris volume in glaucoma patients with type 2 diabetes mellitus by AS-OCT.

Aim: To examine the change of iris volume measured by CASIA2 anterior segment optical coherence tomography (AS-OCT) in glaucoma patients with or without type 2 diabetes mellitus (T2DM) and explore if there is a correlation between hemoglobin A1c (HbA1c) level and iris volume.

Methods: In a cross-sectional study, 72 patients (115 eyes) were divided into two groups: primary open angle glaucoma (POAG) group (55 eyes) and primary angle-closure glaucoma (PACG) group (60 eyes). Patients in each group were separately classified into patients with or without T2DM.

SRT1720 attenuates UVA-induced corneal endothelial damage via inhibition of oxidative stress and cellular apoptosis.

Corneal endothelium is mostly sensitive to oxidative pressure and mitochondrial dysfunction. However, the oxidative-antioxidant mechanism of corneal endothelial cells (CECs) remains partially defined. Silent information regulator 1 (SIRT1) is a well-studied therapeutic target of oxidative damage.

Competitive Adsorption of a Monoclonal Antibody and Nonionic Surfactant at the PDMS/Water Interface.

Interfacial adsorption of monoclonal antibodies (mAbs) can cause structural deformation and induce undesired aggregation and precipitation. Nonionic surfactants are often added to reduce interfacial adsorption of mAbs which may occur during manufacturing, storage, and/or administration. As mAbs are commonly manufactured into ready-to-use syringes coated with silicone oil to improve lubrication, it is important to understand how an mAb, nonionic surfactant, and silicone oil interact at the oil/water interface.