Impact of continuous Triazophos exposure on Labeo rohita: Physiological, biochemical, and histological alterations and IBRv2 index assessment.

Pesticides are commonly used in agriculture and aquaculture. Triazophos, an organophosphate-based pesticide, is widely used in agriculture to control many insect pests. Due to its high photochemical stability and mode of action, Triazophos could persist in the aquatic ecosystem and cause toxic effects on non-target organisms.

Biological-based techniques for real-time water-quality studies: Assessment of non-invasive (swimming consistency and respiration) and toxicity (antioxidants) biomarkers of zebrafish.

Swimming consistency and respiration of fish are recognized as the non-invasive stress biomarkers. Their alterations could directly indicate the presence of pollutants in the water ecosystem. Since these biomarkers are a routine process for fish, it is difficult to monitor their activity manually.

IR-based device to acquire real-time online heart ECG signals of fish (Cyprinus carpio) to evaluate the water quality.

Water quality monitoring is a challenging task due to continuous pollution. The rapid development of engineering technologies has paved the way for the development of efficient and convenient computer-based online continuous water-quality assessment techniques. Techniques based on biological-responses are gaining attention, worldwide.

Developmental toxicity of the emerging contaminant cyclophosphamide and the integrated biomarker response (IBRv2) in zebrafish.

The safety of cyclophosphamide (CP) in the early developmental stages is not studied yet; it is important to study the responses at these stages because they might have relevance to CP-administered humans. We studied the developmental toxicity of CP by analysing physiological, morphological, and oxidative stress, neurotransmission enzymes, gene expression and histological endpoints in zebrafish embryos/larvae. The study lasted for 120 hpf at environmentally relevant concentrations of CP.

Efficiency of hematological, enzymological and oxidative stress biomarkers of Cyprinus carpio to an emerging organic compound (alphamethrin) toxicity.

Alphamethrin is one of the extensively used pyrethroids. Its non-specific mode-of-action might affect the non-target-organisms. Its toxicity data on aquatic organisms are lacking.

Immunotoxicity and Transcriptome Analyses of Zebrafish () Embryos Exposed to 6:2 FTSA.

As a new alternative to perfluorooctane sulfonic acid (PFOS), 6:2 fluorotelomer sulfonic acid (6:2 FTSA) has been widely produced and used in recent years, and its concentration and frequency of detection in the aquatic environment and aquatic organisms are increasing. However, studies of its toxicity in aquatic biological systems are alarmingly scarce, and the relevant toxicological information needs to be improved. In this study, we investigated AB wild-type zebrafish () embryos subjected to acute 6:2 FTSA exposure for immunotoxicity using immunoassays and transcriptomics.

Triphenylmethane dye (CHNO) is potentially a hazardous substance in edible freshwater fish at trace level: toxicity, hematology, biochemistry, antioxidants, and molecular docking evaluation study.

Malachite green (CHNO) is a synthetic dye that is used in textile industries as a colorant and in aquaculture sectors to contain microbial damage. Aquatic contamination of malachite green (MG) has been reported globally. Fish is the highest trophic organism among aquatic inhabitants, highly sensitive to waterborne contaminants (metals, coloring agents, etc.

Real-time determination of water status upon simultaneous zebrafish exposure to sublethal concentrations of CuSO.

Water pollution from commonly occurring contaminants (metals, xenobiotics, etc.) is a serious global problem. Copper is a commonly occurring water contaminant.

IR-Based Novel Device for Real-Time Online Acquisition of Fish Heart ECG Signals.

We developed an infrared (IR)-based real-time online monitoring device (US Patent No: US 10,571,448 B2) to quantify heart electrocardiogram (ECG) signals to assess the water quality based on physiological changes in fish. The device is compact, allowing us to monitor cardiac function for an extended period (from 7 to 30 days depending on the rechargeable battery capacity) without function injury and disturbance of swimming activity. The electrode samples and the biopotential amplifier and microcontroller process the cardiac-electrical signals.

Assessment of eco-toxic effects of commonly used water disinfectant on zebrafish (Danio rerio) swimming behaviour and recovery responses: an early-warning biomarker approach.

Eco-toxicity profiles for commonly used disinfectants were lacking. Available traditional toxicity techniques have some limitations (assessments and ethical issues). Behaviour toxicology is a promising research area towards early warning and non-invasive approaches.

Synthetic organic chemicals (flame retardants and pesticides) with neurotoxic potential induced behavioral impairment on zebrafish (Danio rerio): a non-invasive approach for neurotoxicology.

Behavior responses of organisms can be used as a non-invasive method for neurotoxicology studies since it directly links the nervous system's functioning and biochemical activities. Among different behavioral activities, aquatic organisms' swimming behavior (fitness) is the essential factor for health assessment; thus, it is practiced routinely in neurotoxicological studies. Zebrafish (Danio rerio) are excellent models for neurotoxicology studies.

Responses of Cirrhinus mrigala to second-generation fluoroquinolone (ciprofloxacin) toxicity: Assessment of antioxidants, tissue morphology, and inorganic ions.

Ciprofloxacin drugs are a second-generation fluoroquinolone highly prescribed medication against various bacterial infections in human and aquaculture practices. These drugs are chemically designed to persist in the body long enough to achieve target objectives. Extensive usage has resulted in ciprofloxacin becoming a ubiquitous contaminant in the environment.

Organophosphorus-based chemical additives induced behavioral changes in zebrafish (Danio rerio): Swimming activity is a sensitive stress indicator.

Organophosphorus flame retardants (OPFRs) have been extensively used as chemical additives in polymer based consumer products. Among them, Isopropylphenyl phosphate (IPPP) and tripropyl phosphate (TPP) are predominant, which have potential to cause neuro-toxic effects on non-target organisms. As behavior (swimming activity) response is the first adjustment due to neurotoxic stress on the fitness of fish.

The specification of zebrafish (Danio rerio) heart electrocardiogram index characteristic responses to different types of pollutants.

Water quality was highly affected by common pollutants. Metals, pesticides and small molecules are ubiquitous pollutants. Advancement in engineering technology (computer-based monitoring systems) increased the efficiency of quantifying toxicity of different chemicals in an organism.

Organophosphorus flame retardant induced hepatotoxicity and brain AChE inhibition on zebrafish (Danio rerio).

Organophosphorus flame retardants (OPFRs) are high production volume (HPV) chemicals. Recent reports reveal that OPFRs are ubiquitous in the environment. Unfortunately, the toxicity profiles for OPFRs on organisms remain limited.

Machine-learning-based detection of adaptive divergence of the stream mayfly populations.

Adaptive divergence is a key mechanism shaping the genetic variation of natural populations. A central question linking ecology with evolutionary biology is how spatial environmental heterogeneity can lead to adaptive divergence among local populations within a species. In this study, using a genome scan approach to detect candidate loci under selection, we examined adaptive divergence of the stream mayfly in the Natori River Basin in northeastern Japan.

Biochemical responses of a freshwater fish Cirrhinus mrigala exposed to tris(2-chloroethyl) phosphate (TCEP).

Freshwater fish Cirrhinus mrigala were exposed to tris(2-chloroethyl) phosphate (TCEP) with three different concentrations (0.04, 0.2, and 1 mg/L) for a period of 21 days.

Simultaneous eco-toxicity assessment technique using an online monitoring system: effects of different environmental factors on swimming behavior of zebrafish (Danio rerio).

Environmental factors, such as photoperiod and temperature were the main limiting factors for the survival of organisms in the nature environment. Changes in environmental factors are well predicted but determining their effects on organisms are challenging hot topic in the field of eco-toxicology. Thus, technology based eco-toxicity assessment was focused worldwide.

Biochemical and behavior effects induced by diheptyl phthalate (DHpP) and Diisodecyl phthalate (DIDP) exposed to zebrafish.

Both Diheptyl-phthalate (DHpP) and Diisodecyl-phthalate (DIDP) were used extensively as plasticizers. Recently, their occurrence in the environmental matrices and human body fluids have been reported. Unfortunately, these phthalate congeners are without basic toxicity profiles.

The potential assessment of green alga Chlamydomonas reinhardtii CC-503 in the biodegradation of benz(a)anthracene and the related mechanism analysis.

Benz(a)anthracene (BaA) is a polycyclic aromatic hydrocarbons (PAHs), that belongs to a group of carcinogenic and mutagenic persistent organic pollutants found in a variety of ecological habitats. In this study, the efficient biodegradation of BaA by a green alga Chlamydomonas reinhardtii (C. reinhardtii) CC-503 was investigated.

The continuous physiological changes of zebrafish (Danio rerio) based on metabolism under controlled thallium stress.

In this research, the continuous physiological changes of zebrafish (Danio rerio) in 0.1 μg/L thallium (Tl) in 15 days were investigated. The results showed that Tl(I) stress had a significant positive linear correlation with zebrafish ammonia nitrogen excretion (ANE) (p < 0.

Potential effects of internal physio-ecological changes on the online biomonitoring of water quality: The behavior responses with circadian rhythms of zebrafish (Danio rerio) to different chemicals.

The online biomonitoring of aquatic accidental pollution is very important to realize the assessment of complex toxicity. However, the monitoring results would be affected greatly by the internal physio-ecological changes of test organisms, and circadian rhythms might contribute greatly to this kind of effects. In the present study, the behavior responses of zebrafish (Danio rerio) to different concentrations of Deltamethrin, Atrazine, and Thallium (Tl) in 15 days were investigated using an online behavior monitoring system.

The online monitoring and assessment of thallium stress using oxygen consumption rate and carbon dioxide excretion rate of zebrafish (Danio rerio).

An online monitoring and assessment system of metabolism to measure oxygen consumption rate (OC) and carbon dioxide (CO) excretion rate (CR) of zebrafish (Danio rerio) was used to illustrate changes in stressful states in 15 days' (360 h) 0.1 μg/L Tl exposure. Tl had a significant inhibition on zebrafish OC and CR (p < 0.

Toxic responses of zebrafish (Danio rerio) to thallium and deltamethrin characterized in the electrocardiogram.

The electrocardiogram (ECG) has been widely used to objectively address the physical condition as an index of stress. Though a numerous accounts of investigations on aquatic organisms' ECGs have been made, differentiation of ECG parameters in responding to specific toxic chemicals has not been extensively studied. In this research, it is hypothesized that zebrafish (Danio rerio) ECG parameters would differently respond to different types of pollutants, a heavy metal, thallium (Tl, 0.