Environmental pseudotargeted metabolomics: A high throughput and wide coverage method for metabolic profiling of 1000-year paddy soil chronosequences.

Pseudotargeted metabolomics is achieved by introducing an algorithm designed to choose ions for selected ion monitoring from identified metabolites. This method integrates the advantages of both untargeted and targeted metabolomics. In this study, environmental pseudotargeted metabolomics was established to analyze the soil metabolites, based on microwave assisted derivatization followed by gas chromatography-mass spectrometry analysis.

Black Truffles Affect Physiology and Root-Associated - and -Type Denitrifying Bacterial Communities in the Initial Stage of Inoculation.

Truffles ( spp.) are edible ectomycorrhizal fungi with high economic value. Bacteria in ectomycorrhizosphere soils are considered to be associated with the nutrient uptake of truffles and hosts.

Effects of truffle inoculation on a nursery culture substrate environment and seedling of Carya illinoinensis.

We inoculated Tuber aestivum and Tuber sinoaestivum on Carya illinoinensis to explore the effects of inoculation on host plant growth, enzyme activities, the physicochemical properties of rhizosphere soil, the denitrifying bacterial community in the rhizosphere, and the distribution of mating type genes in the rhizosphere. We found that the Tuber spp. inoculation increased the height of the host plant and that the stem circumference of the host was greater two months after inoculation.

Different maturities drive proteomic and metabolomic changes in Chinese black truffle.

Chinese black truffle (Tuber indicum) is rich in nutrition. However, commercial interests lead to the aroma components and nutrients of T. indicum being greatly affected by overexploitation without consideration of their maturity.

Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.

Truffles are world-renowned premium commodities. Due to their unique aroma and rarity, the price of truffles has always been very high. In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.

shapes S-type denitrifying and ammonia-oxidizing bacterial communities in ectomycorrhizosphere soils.

Background: -type denitrifying bacteria and ammonia-oxidizing bacteria (AOB) play a key role in the soil nitrogen cycle, which may affect the growth and development of underground truffles. We aimed to investigate -type denitrifying bacterial and AOB community structures in the rhizosphere soils of seedlings inoculated with the black truffle () during the early symbiotic stage.

Methods: The seedlings inoculated with or without were cultivated in a greenhouse for six months.

Colonization by Tuber melanosporum and Tuber indicum affects the growth of Pinus armandii and phoD alkaline phosphatase encoding bacterial community in the rhizosphere.

The synthesis of truffle ectomycorrhizae and the ecology of truffle-colonized seedlings in the early symbiotic stage are important for the successful truffle cultivation. In this study, two black truffle species, Tuber melanosporum and Tuber indicum, were selected to colonize Pinus armandii seedlings. 2, 4, 6 and 8 months after inoculation, the growth performance of the host and the rhizosphere soil properties were detected.

Exogenous Nitric Oxide and Phosphorus Stress Affect the Mycorrhization, Plant Growth, and Associated Microbes of Seedlings Colonized by .

In the artificial cultivation of truffles, ectomycorrhizal colonization level, host plant quality, and the associated microbes in the rhizosphere soil are vitally important. To explore the effects of nitric oxide (NO) and phosphorus (P) stress on the early symbiosis of truffles and host plants, different concentrations of exogenous NO donor sodium nitroprusside (SNP) and P were applied to seedlings inoculated with the Chinese black truffle (). The growth of -mycorrhized seedlings and their mycorrhizal colonization rate were investigated.

LC-MS-Based Metabolomic Approach Revealed the Significantly Different Metabolic Profiles of Five Commercial Truffle Species.

Truffles are ascomycetous ectomycorrhizal fungi that have elevated status in the culinary field due to their unique aroma and taste as well as their nutritional value and potential biological activities. , , , , and are five commercial truffle species mainly distributed in Europe or China. In this study, an untargeted metabolomics technology based on an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was applied to analyze the metabolic profiles and variations among these five truffle species.

Shapes the Ectomycorrhizosphere Microbial Communities of .

In this study, eight-month-old ectomycorrhizae of with were synthesized to explore the influence of colonization on the soil properties and the microbial communities associated with during the early symbiotic stage. The results showed that the bacterial richness and diversity in the ectomycorrhizae were significantly higher than those in the control roots, whereas the fungal diversity was not changed in response to colonization. was the dominant taxon (82.

Mycorrhization of with Chinese black truffle significantly altered the host physiology and root-associated microbiomes.

Background: Our aim was to explore how the ectomycorrhizae of an indigenous tree, with a commercial truffle, Chinese black truffle (), affects the host plant physiology and shapes the associated microbial communities in the surrounding environment during the early stage of symbiosis.

Methods: To achieve this, changes in root morphology and microscopic characteristics, plant physiology indices, and the rhizosphere soil properties were investigated when six-month-old ectomycorrhizae were synthesized. Meanwhile, next-generation sequencing technology was used to analyze the bacterial and fungal communities in the root endosphere and rhizosphere soil inoculated with or not.