A novel full-length gene of human ribosomal protein L14.22 related to human glioma.

Background: This study was undertaken to obtain differentially expressed genes related to human glioma by cDNA microarray and the characterization of a novel full-length gene.

Methods: Total RNA was extracted form human glioma and normal brain tissue, and mRNA was used as a probe. The results of hybridization procedure were scanned with the computer system.

[Tumor relevance analysis of a highly conserved gene by using gene microarray hybridization].

Preliminary function research of a highly conserved human gene,which was cloned from human fetal cDNA library during large-scale cDNA sequencing,is illustrated in this article. Bioinformatics analysis indicates that this gene is highly conserved in human, mouse, fruit fly, thaliana and fission yeast. Other bioinformatics analysis implies its relevance with tumors.

cDNA microarray in isolation of novel differentially expressed genes related to human glioma and clone of a novel full-length gene.

Background: This investigation was undertaken to obtain differentially expressed genes related to human glioma using cDNA microarray and the characterization of one novel full-length gene.

Methods: Total RNA was extracted from human glioma tissues and normal brain tissues, and mRNA was used to make probes. After hybridization and washing, the results were scanned using a computer system.

Isolation of novel differentially expressed genes related to human glioma using cDNA microarray and characterizations of two novel full-length genes.

Identification of the genes that are differentially expressed between brain tumor and normal brain tissues is important for understanding the molecular basis of these nerve system tumors and for defining possible targets for therapeutic intervention. This investigation is intended to obtain differentially expressed genes related to human glioma using cDNA microarray. Total RNA was extracted from human glioma specimens and normal brain tissues, and mRNA was obtained by oligotex chromatography.

Cloning and characterization of ARHGAP12, a novel human rhoGAP gene.

Rho GTPase activating proteins (GAPs) stimulate the intrinsic GTP hydrolysis activity of Rho family proteins. Here we report the cloning of two splice variants of a novel gene named ARHGAP12 (access number), which has an ORF of 2541bp. Profilescan search result showed that its putative protein contains five domains: rhoGAP, SH3, PH and two WW domains.