Publications by authors named "Zong-sheng He"
Article Synopsis
- The study aimed to establish a cell mass suspension culture system for Fritillaria cirrhosa to efficiently identify optimal conditions for cell growth and hormone combinations.
- Researchers compared various factors, including inoculum size and different hormone combinations, using MS medium and measured the total alkaloid content in the cultured cell mass.
- Results showed that the cell mass grew significantly faster in suspension culture, yielding higher alkaloid levels than both wild and commercial bulbs, with the best conditions set at an inoculum size of 30 g/L and specific hormone concentrations.
Objective: In order to improving the existent breeds of Fritillaria cirrhosa, increasing its medicinal ingredients and enriching the resources of heredity breeding.
Methods: By using of low-temperature stratification treatment, the seed of Fritillaria cirrhosa completed its after-ripening of physiology and morphology. The induction treatment by different concentrations of colchicine solution and different treatment times for Fritillaria cirrhosa seeds were compared.
Objective: The optimal in vitro culture conditions for the regeneration of Fritillaria cirrhosa were screened.
Methods: Through the in vitro culture of the flowering stage Fritillaria cirrhosa, the effect of illumination, culture temperature, hormone combination and the amount of ribavirin on the regeneration of Fritillaria cirrhosa were studied.
Results And Conclusion: The explant browning level could be apparently reduced if the bulb were cultured under the ray after exposure to proper low-temperature.