Chemical constituents with inhibition against TNF-α from Merrillanthus hainanensis.

Two new steroidal glycosides oxystauntoside A (1) and oxystauntoside B (2), together with sixteen known compounds (3-18) were isolated from the 95% ethanol extract of Merrillanthus hainanensis. Their structures were characterized by extensive spectroscopic analysis including NMR and mass spectra and single crystal X-ray crystallography. The absolute configuration of 1 and 2 were further determined by ECD calculations.

[The affection of antigen presentation ability, which sources from the spleen macrophage of Balb/c mouse during late gestation, on maternal Th2/Th1 type of immune].

Objective: To study the relationship between antigen presentation ability of spleen macrophage and maternal Th2>Th1 immune bias in Balb/c mice during late pregnancy.

Methods: Balb/c mice during late gestation were adopted in our study, and mice of same species in estrus were used as control. With antigen stimulation, the spleen macrophages of Balb/c mice were pulsed as antigen presentation cells (APC).

[Study of fetal lymphocyte of intrahepatic cholestasis of pregnancy].

Objective: To explore effect of fetal lymphocyte on pathogenesis of intrahepatic cholestasis of pregnancy (ICP).

Methods: Twenty pregnant women with ICP and 20 normal pregnant women were enrolled in the study. The single mixed lymphocyte culture/reaction (MLC/MLR) was conducted using inactive lymphocyte obtained from maternal peripheral blood and lymphocyte of cord blood from fetus.

[The role of progesterone in human early pregnancy is mediated by insulin-like growth factors binding protein1-3].

Objective: To determine whether the functional role of progesterone in human early pregnancy is mediated by progesterone receptor(PR) and insulin-like growth factor binding protein 1-3(IGFBP1-3).

Methods: Sample collection was conducted in accordance to the principle of informed content. Decidua and villi were obtained from 48 healthy women in 5-7 weeks of pregnancy, and endometrium in mid-secretory phase was obtained as control.