[Review of chemical constituents, pharmacological effects and clinical applications of Jiaotai Pills and predictive analysis of its quality marker(Q-marker)].

Jiaotai Pills is a traditional medical prescription to treat the incompatibility of heart and kidney. It has the distinctive functions of heart and kidney communication, sedation and hypnosis, anti-anxiety and depression, as well as the improvement of insulin resistance. However, this pill is broadly used to cure insomnia, anxiety, depression, and diabetes in the contemporary clinical trials.

[Establishment of rat heart-kidney insomnia model consistent with traditional Chinese medicine syndrome and its serum metabolomics].

Enzyme-linked immunosorbent assay(ELISA) and metabolomics were used to analyze and compare two animal models of heart-kidney insomnia, in order to explore a more ideal animal model and preliminarily explore the essence of heart-kidney insomnia. Based on the clinical symptoms and disease characteristics of heart-kidney insomnia, the animal model of heart-kidney insomnia was reproduced through intraperitoneal injection with p-chlorophenylalanine(PCPA) and multi-factor interaction. The animal model of disease-syndrome combination was evaluated by behavioral observation, ELISA and metabolomics.

Quality Evaluation of Rhei Radix et Rhizoma Based on SPE and HPLC Characteristic Chromatogram with An Internal Standard.

Objective: To establish a quality evaluation method for Rhei Radix et Rhizoma based on SPE and internal standard HPLC characteristic chromatogram.

Methods: Using water reflux extraction and SPE methods, the total Rhei Radix et Rhizoma anthraquinones were obtained. By HPLC method, the characteristic chromatogram of Rhei Radix et Rhizoma was established,and the qualitative research was performed with DAD and MSn.

[Optimization of Scutellaria baicalensis extraction process by orthogonal experiment combined with pharmacodynamic index].

To optimize the Scutellaria baicalensis extraction process, the filter paper method and the bacteriostatic ratio method were adopted to determine the in vitro bacteriostatic efficacy of water extracts and 60% alcohol extracts from S. baicalensis. The quantitative analysis of multi-components by single-marker (QAMS) was used to determined the contents of four active components, baicalin, wogonoside, baicalein and wogonin.

Effects of Rhein lysinate on H2O2-induced cellular senescence of human umbilical vascular endothelial cells.

Aim: To observe the effect of Rhein lysinate (RHL) on cellular senescence of human umbilical vascular endothelial cells (HUVECs) and elucidate its action mechanism.

Methods: Cell viability was determined using MTT assay. The expression levels of Sirt1 mRNA and protein were measured by RT-PCR and Western blot, respectively.