[Preparation and Characterization of Clopidogrel Bisulfate Liposomes].

Objective: To prepare encapsulated clopidogrel bisulfate (CLP) liposomes so as to deal with the poor water solubility of CLP, and to provide the experimental basis for the development of CLP formulations for intravascular injection.

Methods: CLP-loaded liposomes were prepared using thin film hydration/sonication method and pH gradient active drug loading technology. Then, the morphology, particle size, encapsulation efficiency, drug loading capacity, Zeta potentials and release behavior were characterized.

[Characterization of thermal denaturation process of proteinase K by spectrometry].

The effect of different temperatures on the activity and conformational changes of proteinase K was studied. Methods Proteinase K was treated with different temperatures, then denatured natural substrate casein was used to assay enzyme activity, steady-state and time-resolved fluorescence spectroscopy was used to study tertiary structure, and circular dichroism was used to study secondary structure. Results show with the temperature rising from 25 to 65 degrees C, the enzyme activity and half-life of proteinase K dropped, maximum emission wavelength red shifted from 335 to 354 nm with fluorescence intensity decreasing.

Release test of alliin/alliinase double-layer tablet by HPLC-Allicin determination.

A simple, precise and accurate method was developed and validated for the determination of allicin release from alliin/alliinase double-layer tablets. According to Appendix XC II of Chinese Pharmacopoeia 2010 edition Volume II, a small glass-method was adopted at the rotational speed of 100 r/min using 100 mL phosphate buffer (pH 6.8) as release medium.

[Preparation of a hyaluronic acid modified bovine serum albumin nanoparticle and its anti-tumor effect].

Objective: To prepare hyaluronic acid modified bovine serum albumin nanoparticle, investigate the modification extent, the drug entrapment property and its anti-tumor effect.

Methods: Bovine serum albumin nanoparticles were prepared by desolvation method, then use hyaluronic acid to modify the surface of bovine serum albumin nanoparticle. The decrement of reactive amino groups on the surface of bovine serum albumin nanoparticles was applied to evaluate the modification extent of hyaluronic acid.

Immunotargeting of collagenase on thrombus.

In this study, we aimed to develop a thrombus-targeting delivery system of collagenase bound to a monoclonal antibody, and to investigate the thrombolysis of an immune-conjugate in vitro and in vivo as well as the targeting effect. We prepared the immunizing conjugation of collagenase by the 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDCI) method. In order to conjugate collagenase and a monoclonal antibody, bovine serum albumin was used as a linker, increasing the number of collagenase molecules carried and keeping collagenase and the monoclonal antibody active.

[Preparation and quality evaluation of intravenous tanshinone II (A) emulsion].

Objective: To prepare and evaluate an intravenous emulsion of tanshinone II(A).

Method: Soybean phospholipid mixing with poloxamer 188 was used as emulsifier. Oleic acid and glycerol were used as co-emulsifier and isoosmotic adjusting agent, respectively.

[Determination of ivermectin in pig serum by high performance liquid chromatography].

Objective: To establish a high performance liquid chromatographic method for the determination of micro-amount of ivermectin in pig serum.

Methods: Ivermectin in pig serum was extracted with ethyl acetate after the serum protein was precipitated with 0.05% metaphosphoric acid-methanol in the ratio of 7:3 (V/V).