Publications by authors named "Zong-Mao Chen"

The tea leafhopper, , relies on substrate-borne vibrations for sexual communication and is mainly controlled with chemical pesticides, which poses risks to the environment and food safety. Based on previous studies, we conducted a series of behavioral assays by simultaneous observation of vibration signals and movement to investigate the mating and post-copulation behavior of tea leafhoppers. During mating, the activity of was restricted to dawn and dusk and concentrated on the sixth or seventh mature leaf below the tea bud.

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Article Synopsis
  • The study investigates the olfactory genes in male and female moths, specifically looking at their ability to detect type-II sex pheromone components, which play a crucial role in their communication and reproduction.
  • Researchers identified 150 candidate olfactory genes, including various types of receptors and proteins, with notable findings that certain receptors were more abundant in males, suggesting a specialized role in pheromone detection.
  • In contrast, many other receptors showed higher expression in female antennae, indicating possible functions related to detecting host plant volatiles, highlighting the complexity of olfactory mechanisms in these moths.
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and are two sibling moth species of tea plantations in China. The male antennae of both species can detect shared and specific sex pheromone components. Thus, the primary olfactory center, i.

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  • Sex pheromone-based pest management is important for controlling insect pests like the Scopula subpunctaria tea pest in China, which uses Type II sex pheromones.
  • Researchers analyzed the sex pheromone gland transcriptome of this pest and identified 85,246 transcripts, including 79 Cytochrome P450 monooxygenases, 29 odorant-binding proteins, and 17 chemosensory proteins that likely play roles in pheromone biosynthesis and transportation.
  • Findings suggest specific genes (like SsubCYP341A and SsubOBPs) are highly expressed in the pheromone gland and male antennae, providing crucial information for developing biological control methods by targeting these pheromone pathways.
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For many herbivorous insects, vision is more important than olfaction in the prealighting stage of host habitat location. Tea leafhoppers, (Hemiptera, Cicadellidae), are serious pests that preferentially inhabit the tender leaves of tea plants across China. Here, we investigated whether tea leafhoppers could distinguish foliage colors associated with different leaf ages and use this visual cue to guide suitable habitat location from short distances.

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Background: Methoxyfenozide possesses efficacy against a variety of lepidopteron pests, including the major pests in cauliflower and tea, so it is of great importance to generalize the practical use of methoxyfenozide in the field.

Results: An efficient method was developed and validated in both vegetable matrix and extract-rich matrix (cauliflower and tea) using modified QuEChERS combined with UPLC/MS/MS analysis. The recoveries in cauliflower, made tea and tea shoots ranged from 94.

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Ectropis grisescens Warren 1894 (Lepidopotera: Geometridae) and Ectropis obliqua Prout 1915 (Lepidopotera: Geometridae) are the most destructive chewing pests in China's tea plantations. Ectropis grisescens sex pheromone lures and E. obliqua nucleopolyhedrosis virus (EoNPV) are two species-specific and effective bio-control technologies to control these pests.

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Article Synopsis
  • Two sibling species are significant pests of chewing tea in China, and their sexual reproduction is influenced by differences in sex pheromone detection.
  • Researchers sequenced the transcriptomes of the antennae from male and female individuals to analyze the olfactory genes that identify these pheromones, finding 36 odorant-binding proteins and 52 olfactory receptors.
  • While many genes were highly similar between the two species, differences in gene abundance suggest they use distinct levels of pheromone receptors for recognizing sex pheromones, which may be vital for managing these pest species.
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Jasmonates (JAs), which can exogenously induce anti-herbivore defensive reaction in plants, are important in the field of plant physiology and plant protection. We summarized the ecological effects of exogenous JAs applied on 40 species of plants on various herbivores (such as Lepidoptera and Hemipteran) and their natural enemies in the past 20 years. We systematically genera-lized the research status about the direct and indirect antiherbivore defense induced by JAs, induced systemic defense, induction methods, induced performance in field, and the application status of JAs.

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Chrysoperla sinica is an important natural predator of many notorious agricultural pests. Understanding its olfactory mechanism can help enhance the effectiveness of C. sinica in biological control.

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The tea leafhopper, Empoasca onukii Matsuda, is a serious pest of the tea plant. E. onukii prefers to inhabit vigorously growing tender tea leaves.

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Article Synopsis
  • - Moths can produce sex pheromones in their female pheromone glands and can recognize specific pheromones through their antennae, but the details of how Type II pheromones are made and moved are not well understood.
  • - In this study, researchers created a large database of pheromone gland transcripts from a major tea pest moth in China, identifying several key genes related to biosynthesis and transportation of sex pheromones, including 111 cytochrome P450s, 25 odorant-binding proteins, and 20 chemosensory proteins.
  • - The researchers found that specific genes (one each from CYP, OBP, and CSP families) are highly expressed in the pheromone glands, suggesting they might
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Tea grey geometrid (), a devastating chewing pest in tea plantations throughout China, produces Type-II pheromone components. Little is known about the genes encoding proteins involved in the perception of Type-II sex pheromone components. To investigate the olfaction genes involved in .

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Article Synopsis
  • - Female moths choose specific plants for laying their eggs, which is crucial for the survival of their larvae, and they use specialized sensors on their feet to taste non-volatile chemicals from these plants.
  • - The study focused on the tea geometrid moth, Ectropis obliqua, to identify and analyze the chemosensory structures in females and found various types of proteins involved in taste perception.
  • - Results showed that certain proteins related to taste detection are more abundant in female moths, suggesting these adaptations help with finding suitable host plants for egg-laying, which could lead to new pest control strategies.
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Determination of volatile plant compounds in field ambient air is important to understand chemical communication between plants and insects and will aid the development of semiochemicals from plants for pest control. In this study, a thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) method was developed to measure ultra-trace levels of volatile plant compounds in field ambient air. The desorption parameters of TD, including sorbent tube material, tube desorption temperature, desorption time, and cold trap temperature, were selected and optimized.

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Background: Tea (Camellia sinensis) has long been consumed worldwide for its amazing flavor and aroma. Methyl jasmonate (MeJA), which acts as an effective elicitor among the plant kingdom, could mostly improve the quality of tea aroma by promoting flavor volatiles in tea leaves. Although a variety of volatile secondary metabolites that contribute to aroma quality have been identified, our understanding of the biosynthetic pathways of these compounds has remained largely incomplete.

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Originating in China, tea and tea planting have spread throughout the world since the middle of the Tang dynasty. Now people from 160 countries in the world are accustomed to tea drinking. A brief history of tea's medicinal role in China and its spread to the world are introduced.

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Herbivore-induced plant volatiles have been reported to play a role in the host-searching behavior of herbivores. However, next to nothing is known about the effect of volatiles emitted from tea plants infested by Ectropis obliqua larvae on the behavior of conspecific adults. Here, we found that tea plants infested by E.

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Methyl jasmonate (MeJA) was widely applied in promoting food quality. Aroma is one of the key indicators in judging the quality of tea. This study examined the effect of exogenous MeJA treatment on tea aroma.

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Volatile organic compounds derived from non-host plants, Ocimum basilicum, Rosmarinus officinalis, Corymbia citriodora, and Ruta graveolens, can be used to mask host plant odors, and are repellent to the tea geometrid, Ectropis obliqua. Volatile compounds were collected by headspace absorption, and the components were identified and quantified by using gas chromatography/mass spectrometry. The responses of antennae of female E.

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A comparative study was conducted over three stages on the cleanup efficiency of SPE cartridge Cleanert TPT, newly developed for multigroups of pesticide residues in tea. In Stage I, different SPE cartridges C18, graphite carbon black (GCB), primary secondary amine (PSA), and amino (NH2) were purchased and combined into 12 different sequences. Through the comparative test on cleanup efficiency of 84 representative pesticides in tea, Envi-Carb GCB + PSA with a good cleanup effect was selected.

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  • Polyphenol oxidases (PPOs) are crucial for tea plants' defense against herbivores like the tea geometrid Ectropis obliqua, with increases in PPO activity linked to jasmonic acid (JA) treatment.
  • Caterpillars of Ectropis obliqua showed slower growth and reduced weight gain on JA-treated tea plants compared to control plants, indicating a dose-dependent relationship between JA and caterpillar development.
  • Ectropis obliqua caterpillars can inhibit the production of PPOs in response to mechanical wounding and herbivore regurgitant, suggesting they have adapted to overcome the tea plants' defense mechanisms.
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The tea weevil, Myllocerinus aurolineatus (Voss) (Coleoptera: Curculionidae), is a leaf-feeding pest of Camellia sinensis (O.Ktze.) with aggregative behaviors that can seriously reduce tea yield and quality.

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