Regulation of autophagy and lipid accumulation under phosphate limitation in .

Background: It is known that autophagy is essential for cell survival under stress conditions. Inorganic phosphate (Pi) is an essential nutrient for cell growth and Pi-limitation can trigger autophagy and lipid accumulation in oleaginous yeasts, yet protein (de)-phosphorylation and related signaling events in response to Pi limitation and the molecular basis linking Pi-limitation to autophagy and lipid accumulation remain elusive.

Results: Here, we compared the proteome and phosphoproteome of CGMCC 2.

[Construction of Saccharomyces cerevisiae whole-cell biocatalyst system for conversion miltiradiene].

Tanshinones are the bioactive components of the Chinese medicinal herb Salvia miltiorrhiza, while its biosynthetic pathway remains to be characterized. Rapid identification and characterization of the genes correlated to tanshinones biosynthesis is very important. As one of the intermediates of tanshinones biosynthesis, the ferruginol content is relative low in both root and engineered bacteria.

[Microcalorimetric investigation of two cephalosporins on colon bacteria activity].

The effects of cephradinum and ceftazidime on the metabolism of Escherichia coli (E. coli) DH5alpha was determined by microcalorimetry. The microbial activity was recorded as power-time curves through an ampoule method with a TAM Air Isothermal Microcalorimeter at 37 degrees C.

[Optimized culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides].

Culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides were optimized with single factor and uniform design experiment. The best medium recipe was found with 70 g/L glucose, 0.1 g/L (NH4)2SO4, 0.

[Progress on microbial glyceride biosynthesis and metabolic regulation in oleaginous microorganisms].

Oleaginous microorganisms are small portion of organisms that have ability to accumulate lipids over 20% of their biomass. These species can transform renewable material into microbial oil with similar fatty acid composition to that of plant-based oil. Some organisms may also produce glycerides with high content of poly-unsaturated fatty acids.