Detecting Cell Cycle Stage and Progression in Fission Yeast, Schizosaccharomyces pombe.

We have previously described methods to synchronize cultures of fission yeast, Schizosaccharomyces pombe. In this chapter, we provide methods to detect cell cycle stage in cells and populations of S. pombe.

Cell Cycle Synchrony Methods for Fission Yeast, Schizosaccharomyces pombe.

The fission yeast, Schizosaccharomyces pombe, is a genetically tractable model organism for cell cycle and molecular genetics research. We describe methods to synchronize S. pombe cultures, and the benefits and limitations of each.