Effect of dehydroepiandrosterone on meiotic spindle structure and oocyte quality in mice.

Objectives: Dehydroepiandrosterone (DHEA) has been reported to improve pregnancy chances in women with diminished ovarian reserve (DOR) and to reduce miscarriage rates by 50-80%. This study, therefore, assesses effects of DHEA on number of retrieved oocytes and meiotic spindles.

Materials And Methods: A randomized, prospective, controlled study was conducted on eight groups, four groups of young mice and four elderly.

Study of Tnp1, Tekt1, and Plzf Genes Expression During an in vitro Three-Dimensional Neonatal Male Mice Testis Culture.

Background: In vitro spermatogenesis has a long research history beginning in the early 20th century. This organ culture method was therefore abandoned, and alternative cell culture methods were chosen by many researchers. Here, whether Tnp1, Tekt1, and Plzf, which play a crucial role in spermatogenesis, can be expressed during testis organ culture was assessed.