Modification of a micellar system for amino acid separation by MEKC--application for amino acid profiling in formulations for parenteral use.

The paper proposes a new method for amino acid determination which can be applied for amino acid profiling in solutions for parenteral nutrition. The MEKC method based on a mixed micellar system was developed for the separation of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatized amino acids. Background electrolyte was based on tris-borate buffer with high alkaline pH.

Separation of synthetic food colourants in the mixed micellar system. Application to pharmaceutical analysis.

The paper presents a rapid method for the determination of commonly used synthetic food dyes by micellar electrokinetic capillary chromatography. Detection and separation conditions allowing complete resolution of 15 synthetic food colourants were investigated. The effect of different surfactants on the analytes mobility in relation to their structure was tested.

Application of a capillary electrophoresis method for simultaneous determination of preservatives in pharmaceutical formulations.

Preservatives are used to protect pharmaceutical formulations from microbial attack during the period of administration to the patient. Because of their biological activity, preservatives have to be identified and assayed according to the same rules as apply to active components. A number of methods for separation of preservatives are reported, to account for the heterogeneity of their chemical structures.

Capillary electrophoresis with a diamine EOF modifier as an approach for glutamine dipeptide containing formulations.

Currently, glutamine is thought to be conditionally indispensable amino acid. However, the use of glutamine in clinical practice encounters serious problems related to its limited solubility and instability in water solutions. Therefore, glutamine-containing dipeptides, which have better solubility and stability in the solution, have been introduced into the medical practice.

Development of a capillary electrophoretic method for the analysis of amino acids containing tablets.

Ketosteril is an enteral medicinal product indicated for prevention and therapy in chronic renal insufficiency in connection with a low protein diet. Tablets of Ketosteril contain five essential amino acids like: Lys, His, Thr, Trp, Tyr and another five amino acids in the form of their hydroxy and keto analogues as calcium salts, that are: alpha-ketoleucine, alpha-ketoisoleucine, alpha-ketovaline, alpha-ketophenylalanine and alpha-hydroxymethionine. The composition of Ketosteril tablets is routinely tested with three LC methods.