Publications by authors named "Zoeya A Khan"
Article Synopsis
- The study explores using styrene-maleic acid (SMA) copolymers for extracting and purifying transmembrane proteins while maintaining their native bilayer environment, which addresses drawbacks of traditional detergent methods.
- Different commercially available SMA polymers were tested with three membrane proteins (BmrA, LeuT, and ZipA) to determine their effectiveness in extraction, revealing that specific ratios (2:1 or 3:1 styrene to maleic acid with 7.5-10 kDa size) work best, regardless of protein characteristics.
- SMA 2000 polymer proved most effective for purification and functional stability after one-step affinity purification, but other polymers showed variations that could be useful for different applications.
